Research on Power Load Data Acquisition and Integrated Transmission Systems in Electric Energy Calculation and Detection

This paper presents the crucial area of power load data acquisition with an integrated transmission system for precise calculation and detection of electric energy. With the advances in technology, management and optimization of energy has become critical for sustainability and economic reasons. Thus, we have targeted the cutting-edge methods for data gathering of power load along with its efficient transmission previously reviewed. We scrutinized the current methods and technologies used in power load data acquisition and identified their limitations along with areas of improvements. We followed advanced sensors and measuring devices for data collection employed an integrated transmission system with up-to-the-minute communication protocols and data processing algorithms. These were experimentally verified to improve the accuracy and reliability of the electric energy calculations. The real-world case studies were included for its practical implementations to provide an insight into its impacts. The results of this study provide a maturing outlook along with valuable analysis for electric energy calculation and detection. The system due to its potential for enhancing the energy management and efficiency can have a real-life and profound significance in sustainable and economic handling of the increasing load of energy

NLRP6 Serves as a Negative Regulator of Neutrophil Recruitment and Function During Streptococcus pneumoniae Infection

Streptococcus pneumoniae is an invasive pathogen with high morbidity and mortality in the immunocompromised children and elderly. NOD-like receptor family pyrin domain containing 6 (NLRP6) plays an important role in the host innate immune response against pathogen infections. Our previous studies have shown that NLRP6 plays a negative regulatory role in host defense against S. pneumoniae, but the underlying mechanism is still unclear. The further negative regulatory role of NLRP6 in the host was investigated in this study. Our results showed that NLRP6(-/-) mice in the lung had lower bacterial burdens after S. pneumoniae infection and expressed higher level of tight junction (TJ) protein occludin compared to WT mice, indicating the detrimental role of NLRP6 in the host defense against S. pneumoniae infection. Transcriptome analysis showed that genes related to leukocytes migration and recruitment were differentially expressed between wild-type (WT) and NLRP6 knockout (NLRP6(-/-)) mice during S. pneumoniae infection. Also, NLRP6(-/-) mice showed higher expression of chemokines including C-X-C motif chemokine ligand 1 (CXCL1) and 2 (CXCL2) and lower gene expression of complement C3a receptor 1 (C3aR1) and P-selectin glycoprotein ligand-1 (PSGL-1) which are the factors that inhibit the recruitment of neutrophils. Furthermore, NLRP6(-/-) neutrophils showed increased intracellular bactericidal ability and the formation of neutrophil extracellular traps (NETs) during S. pneumoniae infection. Taken together, our study suggests that NLRP6 is a negative regulator of neutrophil recruitment and function during S. pneumoniae infection. Our study provides a new insight to develop novel strategies to treat invasive pneumococcal infection

A novel variable antibody fragment dimerized by the dHLX peptide with enhanced affinity against amantadine compared to its corresponding scFv antibody

<p>Amantadine (AMA) is an illegally used antiviral drug in the poultry industry, it is necessary to establish a fast, accurate and time-saving detection method for poultry food. The antibody-based immunoassay can achieve fast and accurate requirements. We developed a recombinant antibody-based specificity immunoassay for AMA. In the recombinant antibody, the heavy chain variable region (VH) is connected covalently with the light chain variable region (VL) by the artificial linker. Here, two recombinant antibodies’ single-chain variable fragment (scFv) and scFv-dHLX were constructed and functionally expressed in the periplasm of <i>Escherichia coli</i>. The helix-turn-helix peptide was utilized to dimerize VH and VL similar to the IgG counterpart. The ScFv-dHLX protein showed a higher binding ability and affinity resulting in improvement of <i>in vitro</i> affinity activity over its corresponding scFv. Our results not only indicated scFv-dHLX as an alternative for scFv in analytical application, but also offered a novel and efficient hetero-dimerization pattern of VH and VL leading to enhanced affinity.</p

Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk

A competitive lateral flow immunoassay (LFA) using fluorescent microspheres (FMs) as label was developed for the quantitative detection of chloramphenicol (CAP), thiamphenicol (TAP) and florfenicol (FF) in milk. The limit of detection (LOD) for CAP, TAP and FF in milk was 0.08, 0.8 and 1.9 μg L−1, respectively. The recovery of intra and inter-assay ranged from 87.7% to 107.0% and 82.5% to 112.3%, with corresponding coefficient of variations less than 11.3% and 14.5%, respectively. The sensitivity of the FMs lateral flow assay (FMs-LFA) was comparable to those of the colloidal gold lateral flow assay. A quantitative comparison of the FMs-LFA and LC–MS/MS analysis of milk samples indicated good agreement between the two methods. The FMs-LFA can be used as a reliable, rapid and cost-effective method for food safety analysis