An early diagnostic tool for diabetic peripheral neuropathy in rats

The skin's rewarming rate of diabetic patients is used as a diagnostic tool for early diagnosis of diabetic neuropathy. At present, the relationship between microvascular changes in the skin and diabetic neuropathy is unclear in streptozotocin (STZ) diabetic rats. The aim of this study was to investigate whether the skin rewarming rate in diabetic rats is related to microvascular changes and whether this is accompanied by changes observed in classical diagnostic methods for diabetic peripheral neuropathy. Computer-assisted infrared thermography was used to assess the rewarming rate after cold exposure on the plantar skin of STZ diabetic rats' hind paws. Peripheral neuropathy was determined by the density of intra-epidermal nerve fibers (IENFs), mechanical sensitivity, and electrophysiological recordings. Data were obtained in diabetic rats at four, six, and eight weeks after the induction of diabetes and in controls. Four weeks after the induction of diabetes, a delayed rewarming rate, decreased skin blood flow and decreased density of IENFs were observed. However, the mechanical hyposensitivity and decreased motor nerve conduction velocity (MNCV) developed 6 and 8 weeks after the induction of diabetes. Our study shows that the skin rewarming rate is related to microvascular changes in diabetic rats. Moreover, the skin rewarming rate is a non-invasive method that provides more information for an earlier diagnosis of peripheral neuropathy than the classical monofilament test and MNCV in STZ induced diabetic rats

Assessing service quality of e-waste recycling: A case study from Putrajaya, Malaysia

The quality of recycling services will reflect the effectiveness of recycling performance further improvements. Putrajaya residents were selected as a study site to evaluate the recycling services and assess the ability of the services provided. Data were collected using a stratified random sampling of the households using the questionnaires that have been prior tested for reliability.The critical incident technique (CIT) was used to identify and analyse the satisfaction and dissatisfaction of recycling services. Five successful service constructs identified were a good feeling, secured environment, facilities and efficiency, educational and peer pressure.The paper highlights the form of recovery strategies undertaken by the authorities as perceived by the households

Decreased skin blood flow in diabetic animals.

<p>Plantar skin blood flow was decreased in all diabetic animals (black bars) when compared to controls (white bars). Data is presented as mean ± SEM. *** p< 0.001 (two-way ANOVA with one repeated-measures factor ‘time’ with Bonferroni post-test).</p

Heat hypersensitivity in diabetic animals.

<p>No significant difference was observed between the diabetic animals (dotted line) and controls (continuous line) for the cold plate test (5°C) (<b>A</b>). In contrast to the cold plate test, decreased withdrawal time was observed for the hot plate test (50°C) in diabetic animals (dotted line) when compared to control (continuous line) at all experimental time points (<b>B</b>).</p

Decreased innervation and decreased epidermal thickness in STZ diabetic rats.

<p>Decreased density PGP9.5-IR nerve fibers (<b>A</b>) and average epidermal thickness (<b>F</b>) was demonstrated in the plantar skin of diabetic animals 4, 6, and 8 weeks after induction of diabetes (black bars) when compared to control (white bar). This is illustrated by histological sections of plantar skin in controls (<b>B</b>), and 4 (<b>C</b>), 6 (<b>D</b>), and 8 (<b>E</b>) weeks after induction of diabetes. Data is presented as mean ± SEM. *<i>p</i> < 0.05, **<i>p</i> <0.01 (One-way ANOVA with Tukey post hoc test). E = epidermis, UD = upper dermis, scale bar = 100um.</p

Degeneration of pancreatic tissue in diabetic animals.

<p>Hematoxylin and eosin stained Fig.s of the pancreas (<b>A, B</b>) showing that the cells and the Islets of Langerhans were considerably smaller in the diabetic pancreas (<b>B</b>) compared to control (<b>A</b>). Scale bar 100um.</p

Decreased rewarming rate of the plantar skin after cold exposure.

<p>Progressive decrease in the average skin temperature (degree Celsius) per 120 seconds is shown in diabetic animals when compared to control. Data is presented as mean ± SEM (Unpaired T-test).</p><p>Decreased rewarming rate of the plantar skin after cold exposure.</p

Decreased skin temperature of the plantar hind paw after cold exposure.

<p>Control groups were not significantly different (<b>A</b>). Significant lower skin temperatures were observed in diabetic animals 4 (<b>B</b>), 6 (<b>C</b>), and 8 weeks (<b>D</b>) after the induction of diabetes (dotted line) when compared to controls (continuous line). Data is presented as mean ± SEM. *<i>p</i> < 0.05, **<i>p</i> <0.01, *** <i>p</i>< 0.001 (two-way ANOVA with one repeated-measures factor ‘time’ with Bonferroni post-test).</p