Recomendações para uso de esterqueiras para armazenagem de dejetos de suínos.

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Avaliação do teor de óleo e proteína bruta de genótipos de milho produzidos na Coopervale - Palotina, PR.

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Nitrogen topdressing during heading improve the industrial quality of wheat (Triticum aestivum cv. Mirante) grown with plant growth regulator etyl-trinexapac

O uso de nitrogênio na fase reprodutiva, mesmo em plantas adequadamente supridas de N durante o período vegetativo, pode influenciar positivamente na qualidade industrial do trigo. Entretanto, lavouras adequadamente nutridas podem exibir maior desenvolvimento vegetativo e em consequência disto maior predisposição ao acamamento de plantas. O objetivo do trabalho foi avaliar os efeitos da adubação nitrogenada em cobertura, na fase reprodutiva, em plantas previamente tratadas com o redutor de crescimento etil-trinexapac sobre a qualidade industrial de trigo cv. Mirante. O experimento foi conduzido em campo, durante os anos agrícolas de 2011 e 2012, em Lages, SC. Foram utilizadas duas épocas de aplicação de nitrogênio em cobertura, no afilhamento (estádio vegetativo) e no florescimento (estádio reprodutivo) e o redutor de crescimento etiltrinexapac em duas doses de aplicação, a recomendada (100 g i.a. ha-¹), aplicado quando as plantas estavam no estádio 31, e o dobro desta (100+100 g i.a. ha-¹) aplicado de forma sequencial nos estádios 31 e 32 mais a testemunha. De modo geral o etil-trinexapac não influenciou na qualidade industrial do trigo. Entretanto, a aplicação de nitrogênio no estádio reprodutivo aumentou eficientemente o potencial de panificação da farinha de trigo quanto sua extensibilidade, teor de proteína, glúten úmido, glúten seco e força do glúten.The use of nitrogen in the reproductive phase even in plants adequately supplied with N during the vegetative growing stages can positively influence the wheat industrial quality. However, adequately fertilized crops may exhibit greater vegetative development and in consequence higher incidence of plant lodging. The objective of this study was to evaluate the effect of nitrogen topdressing in the reproductive phase in plants pretreated with the ethyl-trinexapac on industrial quality of wheat grains cv. Mirante. The experiment was carried out in the field conditions at Lages, into 2011 and 2012 growing seasons. The nitrogen topdressing was at tillering (vegetative stage) and flowering (reproductive stage) and plant growth retardant (ethyl-trinexapac) applied at two doses, as commonly used (100 g a.i. ha-¹), on plant shoot at stage 31, and applied twice sequentially in stages 31 plus 32 (100 +100 g a.i. ha-¹). The results pointed that ethyl-trinexapac did not influence the wheat industrial quality. However, the N topdressing, particularly at the reproductive stage effectively increasing to the potential baking of wheat flour as its extensibility, protein content, wet gluten, dry gluten and gluten strength.Facultad de Ciencias Agrarias y Forestale

Composição química de híbridos comerciais de milho testados na Cooperativa Agrícola Mista Vale do Piquiri (Coopervale), Palotina, PR, na safrinha 2001.

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Teores de óleo e proteína bruta de híbridos comerciais de milho testados no Sindicato Rural de São Gabriel do Oeste, MS, na safrinha de 2003.

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Immunohistochemical assessment of Pax8 expression during pancreatic islet development and in human neuroendocrine tumors

The paired box transcription factor Pax8 is critical for development of the eye, thyroid gland as well as the urinary and reproductive organs. In adult, Pax8 overexpression is associated with kidney, ovarian and thyroid tumors and has emerged as a specific marker for these cancers. Recently, Pax8 expression was also reported in human pancreatic islets and in neuroendocrine tumors, identifying Pax8 as a novel member of the Pax family expressed in the pancreas. Herein, we sought to provide a comprehensive analysis of Pax8 expression during pancreogenesis and in adult islets. Immunohistochemical analysis using the most employed Pax8 polyclonal antibody revealed strong nuclear staining in the developing mouse pancreas and in mature human and mouse islets. Astonishingly, Pax8 mRNA in mouse islets was undetectable while human islets exhibited low levels. These discrepancies raised the possibility of antibody cross-reactivity. This premise was confirmed by demonstrating that the polyclonal Pax8 antibody also recognized the islet-enriched Pax6 protein both by Western blotting and immunohistochemistry. Thus, in islets polyclonal Pax8 staining corresponds mainly to Pax6. In order to circumvent this caveat, a novel Pax8 monoclonal antibody was used to re-evaluate whether Pax8 was indeed expressed in islets. Surprisingly, Pax8 was not detected in neither the developing pancreas or in mature islets. Reappraisal of pancreatic neuroendocrine tumors using this Pax8 monoclonal antibody exhibited no immunostaining as compared to the Pax8 polyclonal antibody. In conclusion, Pax8 is not expressed in the pancreas and cast doubts on the value of Pax8 as a pancreatic neuroendocrine tumor marker