HIV infection and mycobacterium tuberculosis drug-resistance among tuberculosis patients in Burkina Faso, West Africa

The aim of this study was to compare the drug-resistance patterns of Mycobacterium tuberculosis strains among pulmonary tuberculosis patients, according to their HIV serostatus, in Burkina Faso. Tuberculosis (TB) patients were classified in new and previously treated cases by using a structured questionnaire. Susceptibility testing to isoniazid, streptomycin, rifampicin and ethambutol was done by the proportion method. Association between HIV-serostatus and drug-resistant TB was assessed with χ2 tests, and the statistical significance was set to

Adaptation des prati ques d’élevage des producteurs de l’Ouest du Burkina Faso face aux contraintes foncières et sanitaires

Dans les exploitations agricoles familiales de l’Ouest du Burkina Faso, l’élevage joue un socio-économique et culturel important. Cependant sa productivité est limitée principalement par des contraintes alimentaires et sanitaires. Cet article analyse ses contraintes et la diversité des pratiques d’alimentation et de soins développées par les producteurs pour y faire face. Les données ont été collectées par la méthode active de recherche participative et par enquête auprès d’un échantillon raisonné de 120 exploitations de la commune de Koumbia. L’analyse de variance (ANOVA) a été utilisée pour comparer les caractéristiques structurelles des agriculteurs, agro-éleveurs et éleveurs et le test de Khi2 pourcomparer leurs pratiques d’élevage.Les résultats montrent que les stratégies d’alimentation des animaux sont plus variées que celles pour les soins. En saison des pluies, la taille élevée du cheptel des éleveurs ne leur permet pas l’accès aux bas-fonds (p < 0,01). Leurs animaux sont essentiellement sur les collines (p < 0,05) et illégalement dans les forêts classées (p < 0,5). En saison sèche, la pâture illégale des forêts classées par leurs animaux s’accentue (p < 0,01) et bon nombre partent en transhumance (p < 0,001). La distribution des résidus de cultures est marquée pour les veaux/velles (p < 0,001) et les vaches allaitantes (p < 0,001) chez les éleveurs, pour les boeufs de trait (p< 0,001) chez les agriculteurs et pour les animaux destinés à la vente chez les agro-éleveurs (p < 0,05). Les éleveurs distribuent plus de tourteau de coton aux vaches allaitantes (p < 0,001). Les agriculteurs vaccinent prioritairement les bovins tandis que les agro-éleveurs et éleveurs ciblent les bovins et les ovins (p < 0,01). Le déparasitage externe touche en premier lieu les bovins chez les agriculteurs et tous les ruminants chez les autres types de producteurs (p < 0,001). Des contraintes alimentaires et sanitaires handicapent l’élevage, dont l’amélioration de la productivité nécessite une synergie d’action des acteurs.Mots clés: exploitation agricole, pratiques d’élevage, alimentation du bétail, santé animale, Afrique de l’OuestEnglish Title: Diversity of breeding practices in western cotton zone of Burkina FasoEnglish AbstractIn the family farm in Burkina Faso West, the breeding have a socio-economic and cultural important part.However its productivity is limited mainly by the food and medical constraints.This article analyzes the constraints and the diversity of animal feeding and health practices developed by producers to make face. The data were collected through participative approach and by  investigation near reasoned sample of 120 farmers in the district of Koumbia. The Anova was used to compare the structural characteristics of the farmers, agro-stockbreeders and stockbreeders and the Khi2 test to compare their breeding practices.The results show that animal feeding strategies varied thanhealth strategies. In rainy season, the high size of the livestock of the stockbreeders does not give to them access underworld (p < 0,01). Their animals are primarily on the hills (p < 0.05) and illegally in the classified forests (p < 0.5). In dry season, the illegal grazing ground of the forests classified by their animals is accentuated (p < 0.01) and good number leave in transhumance (p < 0.001). The distribution of the residues of cultures is shacked with for calves (p < 0.001) and the suckler cows (p<0.001) in the stockbreeders, for oxen of feature (p < 0.001) in the farmers, for the animals to be soldin the agro-stockbreeders (p < 0.05).The stockbreeders distribute more oil cake of cotton to the suckler cows (p < 0.001). The farmers vaccinate the cattle firstly while the agro-stockbreeders and stockbreeders target the cattle and the sheep (p < 0,01). The external déparasitage initially touches the cattle in the farmers and all the ruminants at the other farmer’s types (p < 0.001).Food and medical constraints handicap the breeding.A synergy action of the actors is necessary to improve his productivity.Keywords: farm, breeding practices, animal feed, animal health, West Afric

Importance of the confirmatory assay for the detection of the HBsAg in the epidemiological studies and in the diagnosis of the viral Hepatitis B

Several epidemiological studies have reported high prevalence of HBsAg among pregnant women in Burkina Faso. They used various algorithms, as it is also done for the routine diagnostic. Knowing this antigen carriage rate in such a population or in other clinic attendees is important for the implementation of a national immunisation programme and the monitoring of patients with hepatitis B. Often, the screening tests were not confirmed in spite of the existence of known false positive and false negative results. The aim of this study was to determine a more accurate prevalence of HBsAg, among the pregnant women in Burkina Faso. From October 2006 to January 2007, blood samples were collected from 1139 pregnant women. Each sample was analyzed for HBsAg, using two assays and according to manufacturers’ instructions vis, Hepanostika®HBsAg Uniform II B9 (Bio-Mérieux; France) and HBsAg (V2) Abbott AxSYM® system (Abbott Diagnostics). All the positive samples were tested with a confirmatory neutralization assay- Hepanostika®HBsAg Uniform II B9 Confirmatory (Bio-Merieux). The mean age of the pregnant women was 24.85years [range: 15-45years] and the age range of 20-24 (37%) and 25-29 (25.4%) years were the most represented. The overall rate of HBsAg-positive pregnant women with the two screening assays was 20.9%. The HBsAg detection rate was significantly higher with Hepanostika® UniformII B9 (16.9%) than with HBsAg (V2) AxSYM system assay (12.1%), with

Campylobacter Spp. Epidemiology and Antimicrobial Susceptibility in a Developing Country, Burkina Faso (West Africa)

Data on campylobacteriosis are almost nonexistent in Burkina Faso. In this study conducted from 2006 to 2008 in Ouagadougou, stool specimens and sociodemographic data were collected from 1 246 patients attending the university teaching hospital for enteritis. Stool samples were analyzed for the presence of Campylobacter by the direct culture method on selective mCCDA agar followed by antibiotic susceptibility testing on the isolated strains. The isolation rate of Campylobacter was 2.3%,comprising of the following species C. jejuni (51.8%), C. coli (13.8%), and C. upsaliensis (3.5%). However, 30.9% of the isolates were unidentified. No resistant strain was found to gentamicin. The resistance to amoxicillin+clavulanic acid (3.4%) was lower than those (10.3-34.5%) to the other antibiotics: erythromycin (10.3%), tetracycline (10.3%), ciprofloxacin (13.8%), amoxicillin (24.1%) and ceftriaxone (34.5%), nalidixic acid (34.5%). Significant associations were found between Campylobacter enteritis and contact with animals (P=0.03), and HIV infection (P<0.0001), in contrast to other sociodemographic and seasonal factors. From the data obtained Amoxicillin+clavulanic acid appear to be the first choice for treatment. The implementation of a national program may be helpful in controlling the spread of the disease and the increase of resistance to antibiotics.Keywords: Campylobacter, epidemiology, HIV, drug resistance, Burkina Fas

Prevalence of occult hepatitis B virus infection among blood donors in Ouagadougou, Burkina Faso

Background: In Burkina Faso, the polymerase chain reaction (PCR) assay is not routinely used in the biological qualification of blood donations and this constitutes a risk factor for the transmission of occult hepatitis B virus (HBV) infection during blood transfusion. The objective of this study is to determine the prevalence of occult B infection (OBI) among blood donors for the purposes of improved blood safety in Burkina Faso. Methodology: A descriptive cross-sectional study of 300 HBsAg negative blood donors was conducted in the city of Ouagadougou, Burkina Faso from April to October 2020. Anti-HBc antibody was determined using the BOSON® brand rapid tests. HBV DNA was detected in 75 selected donors by real-time PCR (rt PCR) using the 7500 Fast Real Time PCR assay technique. Results: Of the 300 HBsAg negative donors, 208 (69.3%) were males while 92 (30.7%) were females, with average age of 30.18 years. Anti-HBc antibody was detected in 39 cases (13%). Of the 75 donor samples tested by rt PCR, 3 (4%) were positive for HBV DNA (occult B infection); 2 of which were anti-HBc antibody positive (seropositive OBI) while 1 was anti-HBc antibody negative (seronegative OBI). Conclusion: Given the prevalence of OBI of 4% in this study and its consequences in blood recipients, it appears necessary that in addition to the classic serological markers of hepatitis B, to test for the presence of HBV DNA among blood donors in order to improve transfusion safety. Keywords: Prevalence, Occult B infection; Blood donors, Ouagadougou.   French title: Prévalence de l'infection occulte par le virus de l'hépatite B chez les donneurs de sang à Ouagadougou, Burkina Faso Contexte: Au Burkina Faso, la polymérase chain réaction (PCR) n’est pas utilisée lors de la qualification biologique des dons et cela constitue un facteur de risque de transmission de l’Infection Occulte du virus B (VHB) lors des transfusions sanguines. L’objectif de cette étude était de déterminer la prévalence de l’infection occulte B chez les donneurs de sang en vue d’une meilleure sécurité transfusionnelle. Méthodologie: Une étude transversale prospective, réalisée d’avril à octobre 2020 dans la ville de Ouagadougou incluant 300 donneurs de sang AgHBs négatif. L’anticorps anti HBc a été déterminé par les tests rapides de marque BOSON®. L’ADN du VHB a été recherché chez 75 donneurs par PCR en temps réel (rt PCR) avec le 7500 Fast Real Time PCR. Résultats: Parmi les 300 donneurs AgHBs négatifs, 208 (69,3%) étaient des hommes et 92 (30,7%) des femmes. L’âge moyen était de 30,18 ans. La recherche de l’Ac anti-HBc était positive dans 39 cas (13 %). Parmi les 75 échantillons passés à la rt PCR, 3 (4%) étaient positifs pour l’ADN du VHB. Sur les 3 cas d’ADN VHB positifs, 2 (66,67%) étaient positifs Ac anti HBc et 1 (33,33%) Ac anti HBc négatif. Conclusion: Compte tenu de la prévalence de l’infection occulte B et ses conséquences chez les donneurs de sang et chez les receveurs, il devient nécessaire de rechercher, en plus des marqueurs sérologiques classiques de l’hépatite B, l’ADN VHB pour une meilleure sécurité transfusionnelle. Mots clés: Prévalence, Infection occulte de l’HBV, Donneurs de sang, Ouagadougou &nbsp

Etude épidémiologique, clinique et thérapeutique des hydrocèles dans trois districts sanitaires de la région de Sikasso/Mali: Epidemiological, Clinical and Therapeutic Study of Hydroceles in Three Health Districts in the Sikasso Region / Mali

Context and objective. Hydrocele is one of the most common urogenital manifestations of lymphatic filariasis. It is a common cause of enlarged scrotum in the tropics. This study aims to describe the epidemiological, clinical and therapeutic aspects of hydroceles. Methods. This cross-sectional descriptive study of hydroceles in three endemic filarial Sikasso areas in Mali was conducted from November 2017 to December 2018. The variables studied were: frequency of hydrocele, age of patients, duration of evolution, type of anesthesia, surgical technique, volume, operative time and postoperative results. Results. Three hundred fifty-eight patients were operated on in fourteen months. The frequency of hydrocele‘s surgery was 31%. Their average age was 47.1 years old (extremes 4 months and 94 years). The duration of evolution was 10.7 years (extremes 6 months and 21 years). The right side was the most affected with 44.1% followed by the left side with 31.3%. Hydrocele was bilateral in 19%. Local anesthesia (with xylocaine 2%) was used in 88%. All patients underwent a successful vaginal resection. Conclusion. The hydrocele remains a common urological pathology in these endemic areas. The diagnosis is made after a long period of evolution of the disease. Treatment in outpatient surgery is undertaken using local anesthesia. These hydrocele management campaigns should be encouraged to treat the maximum number of patients. Contexte et objectif. L’hydrocèle constitue l’une des manifestations urogénitales les plus fréquentes de la filariose lymphatique. Elle est une cause fréquente de grosse bourse dans les régions tropicales. L’objectif de cette étude est de décrire les aspects épidémiologiques, cliniques et thérapeutiques des hydrocèles. Méthodes. Il s’agissait d’une étude transversale et descriptive sur les hydrocèles, réalisée entre novembre 2017 et décembre 2018 ; dans trois zones endémiques filariennes dans la région de SIKASSO au Mali. Les variables étudiées étaient : la fréquence de l’hydrocèle, l’âge des patients, la durée d’évolution, le type d’anesthésie, la technique chirurgicale, le volume, le temps opératoire et les résultats postopératoires. Résultats. Trois cent cinquante-huit patients ont été opérés en quatorze mois. L’intervention de l’hydrocèle rendait compte de 31% des activités chirurgicales. Leur âge moyen était de 47,1 ans (extrêmes 4 mois et 94 ans). La durée d’évolution était de 10,7 ans (extrêmes de 6 mois et 21 ans). Le testicule droit était le plus touché (44,1 %) suivi du côté gauche (31,3%). L’hydrocèle était bilatérale dans 19 %. L’anesthésie locale à la xylocaïne 2 % a été réalisée dans 88%. La résection vaginale a été réalisée chez tous les patients avec succès. Conclusion. L’hydrocèle reste une pathologie urologique fréquente en zone d’endémie filarienne. Le diagnostic se fait après une longue durée d’évolution de la maladie. Le traitement en chirurgie ambulatoire réalisée sous anesthésie locale a montré des résultats satisfaisants. Ces campagnes de prise en charge de l’hydrocèle sont à encourager pour pouvoir traiter le maximum de patients

Meningococcal carriage and cerebrospinal meningitis after MenAfriVac mass immunization in Burkina Faso

The aims of this study were to evaluate the impact of conjugate vaccine A, MenAfriVac, on Neisseria meningitidis (Nm) asymptomatic carriage and cerebrospinal meningitis in three health districts (Bogodogo, Kaya, and Dandé) of Burkina Faso. Asymptomatic carriage of Nm was assessed by performing cross-sectional studyrepeated (rounds 1 to 10) before and after introduction of the conjugate vaccine against serogroup A of N. meningitidis (NmA), MenAfriVac. In each round at least 1,500 people were enrolled in each district for a month. Data oncases of meningococcal meningitis in the three studied health districts were collected through meningitides epidemiological surveillance of Burkina Faso.Nm was identified in680 of 23,885 throat swabs before vaccination (2. 84%)withNmYasthe dominant serogroup(1.87%). During the same period (2009 and 2010), 891 cases of suspected meningitis were reported in the three health districts among whom 42 were due toNm (4.71%) withNmX (3.70%) asthe most frequently identified serogroup. After vaccination, Nm was identified in 1117 of 27,245 pharyngeal samples (6.42%); NmX (4.42%) wasthe dominantserogroup. From 2011 to 2013, 965 cases of suspected meningitis were reported in all health facilities in the three studied health districts located in the geographical study area; 91 was due toNm (9.43%) andNmWasthe most commonserogroup(52 cases= 5.38%).After introduction of conjugate vaccine A (MenAfriVac), the NmAserogroup almost disappeared both in asymptomatic carriers and in patients with cerebrospinal meningitis. However the presence of the NmW and NmXserogroups, which appear to have replaced serogroup A, is very worrying with regard to meningitis prevention and control in Burkina Faso. It appears necessary to strengthen surveillance and laboratory diagnosis of the different meningococcal serogroups circulating in Africa.Keywords: meningococcal meningitis, serogroups W and X, meningococcal carriage, MenAfriVac

Residual mother-to-child transmission of HIV in Burkina Faso

Background: Burkina Faso is one of the countries in West Africa most affected by the HIV/AIDS pandemic, despite the implementation of a mother-to-child HIV transmission prevention program as a strategy to reduce the risk of vertical transmission of the disease.Objective: To assess the current risk of mother-to-child transmission of HIV in Burkina Faso.Materials and methods: A prospective study was conducted between December 2014 and July 2016, in the 13 health regions of Burkina Faso. Women who were screened HIV-positive during a prenatal consultation were followed until delivery. Their babies received dry blood spot (DBS) at birth, at week 6 and at 1year, to screen for HIV.Results: Overall, 186 pregnant women were included in the study, with a mean age of 29.17±6.13 years. Of their children, 430 DBS actually received a PCR test, giving a 91.1% PCR implementation rate. After analyses, 6 (1.3%) babies were identified as carriers of HIV1. The newborn’s serological status was associated with delivery pattern (p=0.000), the administration of antiretroviral drugs to the mother after delivery (p=0.0064), the administration of Nevirapine to the newborn at birth (p=0.022), the use of contraceptive methods after delivery (p=0.028) and the presence of breast affections/infections since delivery (p=0.013).Conclusion: The results of our study are encouraging and demonstrate the effectiveness of interventions in the mother-tochild prevention program (PMTCT) for HIV-positive pregnant women can be improved through early initiation of triple therapy in early pregnancy and improved adherence to antiretroviral (ARV) therapy.Keywords: Burkina Faso, HIV/AIDS, mother-to-child transmission, antiretroviral drugs, pregnant wome

Risk factors associated with HIV prevalence in pregnant women in Burkina Faso, from 2006 to 2014

Purpose of the study: To determine the socio-demographic factors influencing the dynamics of HIV prevalence among pregnant women in Burkina Faso.Material and methods: A total of 66,597 pregnant women from the 13 health regions of Burkina Faso were included in this study conducted between 2006 and 2014. Venous blood samples were collected and analyzed for the detection of HIV antibodies according to WHO / UNAIDS strategy II, using the mixed test Vironostika HIV Uniform II Plus O (Bio-Mérieux) and the test discriminating ImmunoCombII HIV-1 & 2 BiSpot (Orgenics). Samples with discordant results between the two tests, as well as those positive to HIV-2 or HIV-1 + 2, were retested with HIV BLOT 2.2 (MP Diagnostics). Sociodemographic data collected from the participants were correlated with their HIV status to determine key risk factors influencing HIV infection prevalence in Burkina Faso.Results: Sociodemographic data showed that the study population consisted mainly of married women (91.2%) at their first pregnancy (27.1%) with a large majority of them being housewives (86.2%) who did not attend any form of schooling (69.4%). About 88.4% had stayed longer than a year in the health region where they initially participated in the study and 55.8% were between 20 and 29 years of age. Overall HIV prevalence significantly dropped from 2.7 % in 2006 to 1.3% in 2014. However HIV seroprevalence in this study has varied significantly according to socio-demographic characteristics including marital status, parity, occupation, education, age group and the length of stay in the women's health community (p <0.0001). Factors sustaining HIV transmission included the status of being unmarried (OR=1.67 [1.42-1.97]), primigest (OR=1.64 [1.41-1.89]), having other occupations except being student (OR = 1.68 [1.20-2.33]), aged between 20-49 years (OR=3.14 [2.51-3.93]) and the duration of stay less than a year in their locality (OR=5.33 [4.61-10.16]) and these factors were identified as main risk factors associated with HIV prevalence.Conclusion: Burkina Faso remains among the countries with concentrated epidemics despite a significant reduction in the prevalence observed in this study. The inclusion of identified risk factors in the national HIV program could improve the quality of the response to the epidemic.Keywords: HIV-Pregnant Women-Risk Factors-Burkina Fas