3,195 research outputs found
Time to Progress: the Impact of Temporal Ownership on Time Perception
Time is often linguistically portrayed either in a narrative or possessive framing. Building on this tendency, the authors demonstrate that communicating time in possession framing increases perceived ownership and feelings of responsibility towards making use of time. The heightened responsibility leads consumers to minimize time loss, but maximize time investment
Port Capacity Evaluation Formula for General Cargo
AbstractThe objective of this research is to study the evaluation method of berth capacity for general cargo in port. Specially, this study discussed the evaluation formula for general cargo berth capacity. It described the traditional evaluation method and new evaluation method with a sample case using real data of the general cargo berth. The results of the study revealed some problems for traditional evaluation method of general cargo handling capacity. Traditional calculation method does not reflect real situation for the general cargo berth capacity. Also, it does not reflect individual circumstances in terms of input parameter like berth occupancy rate, an annual working day and daily working hour. Finally, this study recommended the applicable and effective formula to reflect real situation on ports which can give direction for developing evaluation of formula for general cargo port capacity
Lifespan extension and increased resistance to environmental stressors by N-Acetyl-L-Cysteine in Caenorhabditis elegans
OBJECTIVE: This study was performed to determine the effect of N-acetyl-L-cysteine, a modified sulfur-containing amino acid that acts as a strong cellular antioxidant, on the response to environmental stressors and on aging in C. elegans. METHOD: The survival of worms under oxidative stress conditions induced by paraquat was evaluated with and without in vivo N-acetyl-L-cysteine treatment. The effect of N-acetyl-L-cysteine on the response to other environmental stressors, including heat stress and ultraviolet irradiation (UV), was also monitored. To investigate the effect on aging, we examined changes in lifespan, fertility, and expression of age-related biomarkers in C. elegans after N-acetyl-L-cysteine treatment. RESULTS: Dietary N-acetyl-L-cysteine supplementation significantly increased resistance to oxidative stress, heat stress, and UV irradiation in C. elegans. In addition, N-acetyl-L-cysteine supplementation significantly extended both the mean and maximum lifespan of C. elegans. The mean lifespan was extended by up to 30.5% with 5 mM N-acetyl-L-cysteine treatment, and the maximum lifespan was increased by 8 days. N-acetyl-L-cysteine supplementation also increased the total number of progeny produced and extended the gravid period of C. elegans. The green fluorescent protein reporter assay revealed that expression of the stress-responsive genes, sod-3 and hsp-16.2, increased significantly following N-acetyl-L-cysteine treatment. CONCLUSION: N-acetyl-L-cysteine supplementation confers a longevity phenotype in C. elegans, possibly through increased resistance to environmental stressors
Biportal Endoscopic Lumbar Interbody Fusion: Review of Current Evidence and the Literature
Several studies report that biportal endoscopic lumbar interbody fusion (ELIF) demonstrate various advantages such as favorable clinical outcomes, less postoperative back pain, and shorter hospital stays compared to open PLIF/MIS TLIF. Even though biportal ELIF has demonstrated several advantages, limitations are also associated with the technique, including longer operation time, limited indications, and a lack of multicenter randomized controlled trials that directly compare other fusion techniques with biportal ELIF. The objective of this review was to evaluate previously published articles and summarize current evidence on biportal ELIF regarding its indications, surgical technique, complications and comparative results with open PLIF/MIS TLIF
Selenocysteine modulates resistance to environmental stress and confers anti-aging effects in C. elegans
OBJECTIVE: The free radical theory of aging suggests that cellular oxidative damage caused by free radicals is a leading cause of aging. In the present study, we examined the effects of a well-known anti-oxidant amino acid derivative, selenocysteine, in response to environmental stress and aging using Caenorhabditis elegans as a model system. METHOD: The response to oxidative stress induced by H2O2 or ultraviolet irradiation was compared between the untreated control and selenocysteine-treated groups. The effect of selenocysteine on lifespan and fertility was then determined. To examine the effect of selenocysteine on muscle aging, we monitored the change in motility with aging in both the untreated control and selenocysteine-treated groups. RESULTS: Dietary supplementation with selenocysteine significantly increased resistance to oxidative stress. Survival after ultraviolet irradiation was also increased by supplementation with selenocysteine. Treatment with selenocysteine confers a longevity phenotype without an accompanying reduction in fertility, which is frequently observed in lifespan-extending interventions as a trade-off in C. elegans. In addition, the age-related decline in motility was significantly delayed by supplementation of selenocysteine. CONCLUSION: These findings suggest that dietary supplementation of selenocysteine can modulate response to stressors and lead to lifespan extension, thus supporting the free radical theory of aging
Influence of cultivation parameters or supplement on product qualities and culture performances during perfusion
Perfusion processes have been developed with technological advances in single-use bioreactor and cell retention device. Perfusion has advantages such as high cell density culture in compact facilities and media change or culture parameter shifts for protein production with desired qualities. Single use bioreactor (200L or 1000L) with ATF System was used for the clinical production. For the process development, the scale-down model was established with lab-scale (2L) bioreactor with ATF2.
A recombinant CHO cell line producing a fusion protein was cultivated using in-house serum-free media. Influence of insulin (0~3mg/L) on qualities was investigated in the established perfusion process using in-house serum-free medium and 2L scale-down model. The results showed that low concentration of insulin enhanced O-glycosylation and -2 charged N-glycan of fusion protein. Temperature (30~34oC) conditions, under no addition of insulin, were investigated to evaluate effect on qualities. High temperature enhanced O-glycosylation and -2 charged N-glycan of fusion protein.
To evaluate interaction among culture parameters (Temperature, pH, and Dissolved Oxygen) in the perfusion process, Central Composite Inscribed (CCI) was selected as design of experiment. 20 perfusion cultures were carried out in the 2L scale-down model. The results showed each parameter and interactions among parameters had an effect on qualities and culture performances
Reconstructive challenge of dermatofibrosarcoma protuberans in the female breast
Dermatofibrosarcoma protuberans is an uncommon locally aggressive malignant neoplasm that most frequently appears in the trunk, followed by the extremities, head, and neck. But occurrence in the breast is extremely rare. We present a case of a 28-year-old woman, who had a history of trauma 5 years previously and excision 1 year before presentation at our clinic. We performed wide excision, together with microscopic and immunohistochemical analysis. No postoperative oncologic treatment was used and she remains disease-free 1 year after the surgery without any tumor recurrence. Here, we report a case of dermatofibrosarcoma protuberans in the female breast and present a detailed discussion of the diagnosis and treatment with reference to available literatures
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