433 research outputs found
Elicitor-Induced Spruce Stress Lignin (Structural Similarity to Early Developmental Lignins)
Detoxification of Formaldehyde by the Spider Plant (Chlorophytum comosum L.) and by Soybean (Glycine max L.) Cell-Suspension Cultures
Effects of abiotic stress on gene transcription in European beech: ozone affects ethylene biosynthesis in saplings of Fagus sylvatica L.
Einfluss der Hackschnitzellagerung auf die Abgabe von flüchtigen Säuren aus daraus hergestellten Spänen und Fasern
The Probability of a Horizontal Gene Transfer from Roundup Ready® Soybean to Root Symbiotic Bacteria: A Risk Assessment Study on the GSF Lysimeter Station
Three-dimensional histological characterization of the placental vasculature using light sheet microscopy
The placenta is the first embryonic organ, representing the connection between the embryo and the mother, and is therefore necessary for the embryo’s growth and survival. To meet the ever-growing need for nutrient and gas exchange, the maternal spiral arteries undergo extensive remodeling, thus increasing the uteroplacental blood flow by 16-fold. However, the insufficient remodeling of the spiral arteries can lead to severe pregnancy-associated disorders, including but not limited to pre-eclampsia. Insufficient endovascular trophoblast invasion plays a key role in the manifestation of pre-eclampsia; however, the underlying processes are complex and still unknown. Classical histopathology is based on two-dimensional section microscopy, which lacks a volumetric representation of the vascular remodeling process. To further characterize the uteroplacental vascularization, a detailed, non-destructive, and subcellular visualization is beneficial. In this study, we use light sheet microscopy for optical sectioning, thus establishing a method to obtain a three-dimensional visualization of the vascular system in the placenta. By introducing a volumetric visualization method of the placenta, we could establish a powerful tool to deeply investigate the heterogeneity of the spiral arteries during the remodeling process, evaluate the state-of-the-art treatment options, effects on vascularization, and, ultimately, reveal new insights into the underlying pathology of pre-eclampsia
Increased Anion Channel Activity Is an Unavoidable Event in Ozone-Induced Programmed Cell Death
Ozone is a major secondary air pollutant often reaching high concentrations
in urban areas under strong daylight, high temperature and stagnant
high-pressure systems. Ozone in the troposphere is a pollutant that is
harmful to the plant. generation by salicylic and abscisic acids.
Anion channel activation was also shown to promote the accumulation of
transcripts encoding vacuolar processing enzymes, a family of proteases
previously reported to contribute to the disruption of vacuole integrity
observed during programmed cell death.-induced
programmed cell death. Because ion channels and more specifically anion
channels assume a crucial position in cells, an understanding about the
underlying role(s) for ion channels in the signalling pathway leading to
programmed cell death is a subject that warrants future investigation
In Vivo Biotransformation of 3,3′,4,4′-Tetrachlorobiphenyl by Whole Plants−Poplars and Switchgrass
Polychlorinated biphenyls (PCBs) are widely distributed persistent organic pollutants. In vitro research has shown that plant cell cultures might transform lower chlorinated congeners to hydroxylated PCBs, but there are few studies on in vivo metabolism of PCBs by intact whole plants. In this research, poplar plants (Populus deltoides × nigra, DN34) and switchgrass (Panicum vigratum, Alamo) were hydroponically exposed to 3,3′,4,4′-tetrachlorobiphenyl (CB77). Metabolism in plants occurred rapidly, and metabolites were detected after only a 24 h exposure. Rearrangement of chlorine atoms and dechlorination of CB77 by plants was unexpectedly observed. In addition, poplars were able to hydroxylate CB77 and the metabolite 6-hydroxy-3,3′,4,4′-tetrachlorobiphenyl (6-OH-CB77) was identified and quantified. Hybrid poplar was able to hydroxylate CB77, but switchgrass was not, suggesting that enzymatic transformations are plant specific. Sulfur-containing metabolites (from the action of sulfotransferases) were investigated in this study, but they were not detected in either poplar or switchgrass
Immunocytochemical determination of the subcellular distribution of ascorbate in plants
Ascorbate is an important antioxidant in plants and fulfills many functions related to plant defense, redox signaling and modulation of gene expression. We have analyzed the subcellular distribution of reduced and oxidized ascorbate in leaf cells of Arabidopsis thaliana and Nicotiana tabacum by high-resolution immuno electron microscopy. The accuracy and specificity of the applied method is supported by several observations. First, preadsorption of the ascorbate antisera with ascorbic acid or dehydroascorbic acid resulted in the reduction of the labeling to background levels. Second, the overall labeling density was reduced between 50 and 61% in the ascorbate-deficient Arabidopsis mutants vtc1-2 and vtc2-1, which correlated well with biochemical measurements. The highest ascorbate-specific labeling was detected in nuclei and the cytosol whereas the lowest levels were found in vacuoles. Intermediate labeling was observed in chloroplasts, mitochondria and peroxisomes. This method was used to determine the subcellular ascorbate distribution in leaf cells of plants exposed to high light intensity, a stress factor that is well known to cause an increase in cellular ascorbate concentration. High light intensities resulted in a strong increase in overall labeling density. Interestingly, the strongest compartment-specific increase was found in vacuoles (fourfold) and in plastids (twofold). Ascorbate-specific labeling was restricted to the matrix of mitochondria and to the stroma of chloroplasts in control plants but was also detected in the lumen of thylakoids after high light exposure. In summary, this study reveals an improved insight into the subcellular distribution of ascorbate in plants and the method can now be applied to determine compartment-specific changes in ascorbate in response to various stress situations
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