4 research outputs found
Anemia and Hyperplastic Gastritis in a Giant Anteater (Myrmecophaga tridactyla) due to Physaloptera magnipapilla Parasitism
A free living female adult of Myrmecophaga tridactyla was captured in an area of deforestation in Salta Province of northwest Argentina, for subsequent relocation in Iberá Park. Three days after entering the quarantine it died with clinical signs of anorexia and ascites. It presented severe hypochromic microcytic anemia, hypoproteinemia and increase of CPK, AST and LDH on blood analysis. Grossly, there were ascites, hydrothorax, pulmonary congestion and severe catarrhal hyperplasic gastritis. The gastric mucosa was diffusely hyperemic with two clusters of numerous Physaloptera magnipapilla attached in the fundic region. Fundic mucosa was irregular with convoluted surface and covered with abundant brown-yellow catarrhal exudates. Microscopically, the gastric mucosa had well described punctate areas (pit-like structures) at points of P. magnipapilla attachment. Additionally, there were mucous hyperplasia of gastric glands and focal fibrosis with scarce infiltration of plasma cells, lymphocytes, multinucleated giant cells and eosinophils in the underlying lamina propria. This report describes a case of anemia and hyperplastic gastritis due to Physaloptera magnipapilla parasitism in a free-living Myrmecophaga tridactyla.Fil: Lertora, Walter Javier. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Departamento de Patología General; ArgentinaFil: Montenegro Maria. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Departamento de Patología General; ArgentinaFil: Mussart, Norma Beatriz. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Villordo, Gabriela Ines. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias. Departamento de Morfología Aplicada. Anatomia Comparada I Parte; ArgentinaFil: Sanchez Negrette, Marcial. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Departamento de Patología General; Argentin
Bubaline milk with a high content of trans fatty acids increased cholesterolemia and tended to aggravate atherosclerosis in rabbits
Búfalas suplementadas con una mezcla de aceites de girasol + pescado pueden reducir en la leche los niveles de ácidos grasos saturados (AGS), considerados hipercolesterolémicos y pro-aterogénicos, e incrementar los ácidos grasos trans-11 18:1 y cis-9,trans-11 18:2, con propiedades anti-aterogénicas. Este trabajo comparó los efectos del consumo de dos leches bubalinas, con baja y alta relación de ácidos grasos trans (AGt)/AGS, sobre el lipidograma y el desarrollo de ateroesclerosis inducidos por colesterol en conejos. Veinte conejos neozelandeses, machos, fueron aleatoriamente separados en un grupo control (n=10) que recibió leche con baja relación AGt/AGS (5,3% de AGt y 67,12% de AGS) obtenida de búfalas en sistema pastoril; y un grupo alto trans (n=10) que recibió leche con alta relación AGt/AGS (25,84% de AGt y 45,89% de AGS) obtenida de búfalas con suplementación lipídica en su dieta. La experiencia duró 75 días y, a partir del día 15, todos los conejos fueron desafiados con 0,93 g de colesterol/día, vía oral, a fin de inducir lesiones ateroescleróticas. Los conejos consumieron de manera voluntaria 9293,13 ml y 9930 ml de leche con baja y alta relación AGt/AGS, respectivamente (p 0,404). Los conejos que consumieron leche con alta relación AGt/AGS registraron un leve incremento significativo (p 0,049) del colesterol total (6,08 g/l), cuando se comparó con el grupo control (5,58 g/l). No se detectaron diferencias entre grupos en el colesterol LDL (p 0,073), colesterol HDL (p 0,078) y triglicéridos (p 0,174). Las lesiones ateroescleróticas fueron más extensas en los conejos que consumieron leche con alta relación AGt/AGS, aunque sin significación estadística (p>0,05) cuando se comparó el control. En conclusión, la leche bubalina obtenida con la suplementación lipídica referida en este estudio, incrementó la colesterolemia y tendió a agravar la ateroesclerosis en conejos. Dichos efectos, posiblemente estén relacionados con el alto contenido de AGt de esta leche.Bubaline milk with a high content of trans fatty acids increased cholesterolemia and tended to aggravate atherosclerosis in rabbits. Rev. Vet. 30: 1, 32-38, 2019. Buffalo heifers supplemented with a mixture of sunflower oil + fish can produce milk with low level saturated fatty acids (SFA, which are considered hypercholesterolemic and pro-atherogenic), and increase the synthesis of trans-11 18: 1 and cis-9, trans-11 18: 2 fatty acids (with anti-atherogenic properties). This work compared the effects of the administration to rabbits of two bubaline milk, with low and high trans/saturated fatty acids (tFA/SFA) ratio, on the development of atherosclerosis induced by cholesterol. Twenty New Zealand rabbits, males, were randomly separated into a control group (n=10) that received milk with low tFA/SFA ratio (5.3% tFA and 67.12% SFA) obtained from grazing buffalos; and a high trans group (n = 10) that received milk with high tFA/SFA ratio (25.84% tFA and 45.89% SFA) obtained from buffalos with lipid supplementation in their diet. The experience lasted 75 days and, from day 15, all rabbits were challenged with 0.93 g of cholesterol/day orally, to induce atherosclerotic lesions. The rabbits voluntarily consumed 9293.13 ml and 9930 ml of milk with low and high tFA/SFA ratio, respectively (p 0.404). Rabbits that consumed milk with a high tFA/SFA ratio increased (p 0.049) serum levels of total cholesterol (6.08 g/l), compared to the control group (5.58 g/l). No differences were detected between groups in LDL cholesterol (p 0.073), HDL cholesterol (p 0.078) and triglycerides (p 0.174). Atherosclerotic lesions were more extensive in rabbits that consumed milk with a high tFA/SFA ratio, although without statistical significance (p> 0.05) compared to rabbits that consumed milk with low tFA/SFA ratio. In conclusion, bubaline milk obtained from animals with lipid supplementation contributed to increase cholesterolemia and tended to worsen atherosclerosis in rabbits. These effects may be related to the high content of tFA of this milk.Fil: Lertora, Walter Javier. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; ArgentinaFil: Villordo, Gabriela Ines. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; ArgentinaFil: Mussart, Norma Beatriz. Universidad Nacional del Nordeste. Facultad de Cs.veterinarias. Departamento de Clinica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Catuogno, María Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; ArgentinaFil: Sanchez Negrette, Marcial. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentin
Reduced development of experimental colon cancer in rats fed with buffalo milk
La leche bovina contiene elementos potencialmente capaces de inhibir la carcinogénesis; a su vez, la leche bubalina tiene un alto valor debido a su mayor concentración de sólidos totales, grasa, proteína y lactosa. El objetivo del presente trabajo consistió en determinar el efecto anticancerígeno de la leche bubalina sobre el desarrollo de tumores y focos de criptas displásicas (FCD) en colon de ratas tratadas con el cancerígeno 1,2-dimetilhidrazina. Las ratas (n= 78) fueron divididas al azar en lotes que recibieron agua o leche, con y sin tratamiento con el cancerígeno, durante 123 y 240 días. Al final de la experiencia se realizó en cada animal el análisis macro y microscópico del intestino grueso, determinándose presencia, número y ubicación de tumores y de FCD. Los resultados demostraron disminución significativa del número de tumores (60%) y de FCD (62,5%) en los lotes que recibieron leche al compararlos con los que recibieron agua. Estos resultados permiten demostrar que la leche de búfala disminuye tanto el desarrollo de tumores como de lesiones displásicas de colon en un modelo de carcinogénesis experimental del intestino grueso.Bovine milk contains elements with potential to inhibit carcinogenesis; in turn, buffalo milk is of a high value as it contains more total solids, fat, protein and lactose compared to the former. The objective of this study was to determine the anticancer effect of buffalo milk on the development of tumors and dysplastic crypt foci (DCF) in colon of rats treated with the carcinogen 1,2-dimethylhydrazine. Rats (n= 78) were randomly divided into lots that received water or milk, with and without treatment with the carcinogen, during 123 and 240 days. At the end of the experiment macro and microscopic analysis of the large intestine were performed on each animal, determining the presence, number and location of tumors and DCF. The results showed significant reduction in tumor number (60%) and DCF (62.5%) in lots that received milk compared to those that received water. These results allow us to demonstrate that buffalo milk reduces both the development of tumors and dysplastic lesions of colon carcinogenesis in an experimental model of the large intestine.Fil: Ramírez, Gabriela Verónica. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Villordo, Gabriela Ines. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; ArgentinaFil: Montenegro, María A.. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; ArgentinaFil: Catuogno, María Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; ArgentinaFil: Sanchez Negrette, Marcial. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentin
Equine laminitis: Bites by Bothrops spp cause hoof lamellar pathology in the contralateral as well as in the bitten limb
The envenoming caused by Bothrops snakebite includes local symptoms, such as pronounced edema, hemorrhage, intense pain, vesicles, blisters and myonecrosis. The principal systemic symptom consists in the alteration of blood clotting, due to fibrinogen consumption and platelet abnormalities. The horses involved in this study had this symptomatology and one of them exhibited symptoms consistent with laminitis in the bitten and in the contralateral limbs. Laminitis lesions were characterized by separation of the hoof lamellar basement membrane (BM) from basal cells of the epidermis. These results demonstrated that Bothrops snake venom can induce acute laminitis. We conclude that components of the venom, probably metalloproteinases, cause severe lesions in the hoof early in the envenoming process. Antivenom therapy must be initiated as soon as possible in order to prevent complications, not only to save the life of an envenomed horse, but also to avoid the dysfunctional sequels of laminitis. (c) 2006 Elsevier Ltd. All rights reserved