MOESM1 of The elongation factor eEF3 (Yef3) interacts with mRNA in a translation independent manner

Additional file 1: Table S1. Proteins identified in the mass spectroscopy analysis. List of 134 genes for which at least two identified unique peptides and FDR<0.01 were detected in the LC-MS/MS analysis. The table includes columns with the gene name, protein molecular weight, the number of peptides that were identified and whether there is experimental evidence for it to bind RNA

Locally translated mTOR controls axonal local translation in nerve injury

How is protein synthesis initiated locally in neurons? We found that mTOR (mechanistic target of rapamycin) was activated and then up-regulated in injured axons, owing to local translation of mTOR messenger RNA (mRNA). This mRNA was transported into axons by the cell size-regulating RNA-binding protein nucleolin. Furthermore, mTOR controlled local translation in injured axons. This included regulation of its own translation and that of retrograde injury signaling molecules such as importin β1 and STAT3 (signal transducer and activator of transcription 3). Deletion of the mTOR 3' untranslated region (3'UTR) in mice reduced mTOR in axons and decreased local translation after nerve injury. Both pharmacological inhibition of mTOR in axons and deletion of the mTOR 3'UTR decreased proprioceptive neuronal survival after nerve injury. Thus, mRNA localization enables spatiotemporal control of mTOR pathways regulating local translation and long-range intracellular signaling