Partitioning of fibrinolytic protease from Bacillus sp. UFPEDA 485 by aqueous two-phase systems using PEG/sodium sulfate

XI Reunião Regional Nordeste da SBBq | 4th International Symposium in Biochemistry of Macromolecules and BiotechnologyThe fibrinolytic protease produced by bacteria of the genus Bacillus has attracted large interest in the pharmaceutical industry as a promising alternative in thrombolytic therapy due to their effectiveness in degrading fibrin, its production requiring the development of an efficient recovery process. Aqueous two-phase system (ATPS) have been recognized as an efficient and economical process for recovering enzymes due to their relative ease and low cost. The purpose of this work was to study the partition of fibrinolytic protease produced by Bacillus sp. UFPEDA 485 in a ATPS composed by Polyethylene glycol (PEG) and sodium sulfate using factorial design. The fibrinolytic protease production occurred in liquid culture medium containing 2% soy flour, pH 7.2, 150 rpm at 37 °C for 48 hours. To study the partitioning, was used a 23 full factorial design with four replicates at the central with the purpose of evaluating the effects and interactions of the independent variables: PEG molar mass (MMPEG), PEG concentration (CPEG) and sodium sulfate concentration (CNa2SO4) on the response variable: partition coefficient (K) of the fibrinolytic enzyme. In all the runs the enzyme partitioned to the top phase, indicating a significant interaction between the protein and the PEG. The best result was obtained at the central point, using MMPEG 6000 g/mol, CPEG 24 % and CNa2SO4 11,6 %. The partitions coefficients ranged between K = 327.63 and 2879.38. According to the results, the ATPS composed of PEG/sodium sulfate proved to be a promising method to extraction fibrinolytic protease.CAPES e CNPqinfo:eu-repo/semantics/publishedVersio

A review on some results on local conservation laws for certain evolution equations

In this work we revisit some recent results on conservation laws for a class of fifth-order evolution equations up to fifth-order.Neste trabalho revisitamos alguns resultados recentes sobre leis de conservação de uma classe de equações evolutivas até quinta ordem.10911

Sistema reprodutivo do Ipê-Branco: Tabebuia roseo-alba (Ridley) Sandwith (Bignoniaceae) Breeding system of the White Trumpet Tree: Tabebuia roseo-alba (Ridley) Sandwith (Bignoniaceae)

Estudos sobre sistemas reprodutivos têm indicado o predomínio da autoincompatibilidade de ação tardia (AIT) em Bignoniaceae, embora poucas espécies tenham sido investigadas e ocorram outros tipos de sistemas reprodutivos na família. O presente estudo objetivou determinar o sistema reprodutivo de T. roseo-alba através de experimentos de polinizações controladas, análise histológica dos eventos posteriores à polinização, verificação do desenvolvimento in situ dos tubos polínicos e testes de germinação de sementes. Apesar de os tubos polínicos penetrarem e fecundarem a maioria dos óvulos em pistilos autopolinizados, o aborto de 100% dos mesmos foi verificado e, embora sua abscisão tenha ocorrido entre o quarto e o sexto dia após o início da antese, observou-se um ligeiro crescimento dos óvulos e do ovário precedendo a abscisão, porém inferior ao crescimento nos pistilos submetidos à polinização cruzada. A endospermogênese inicial e a formação do tubo proembriônico também foram mais lentas nos pistilos autopolinizados. A longevidade dos pistilos autopolinizados foi maior que a de pistilos não polinizados, e a taxa de germinação de sementes foi de 93%, sendo todas as sementes monoembriônicas. Os resultados demonstram que T. roseo-alba é espécie auto-estéril, destituída de poliembrionia e que apresenta AIT pós-zigótica.<br>Breeding system studies have indicated the predominance of late-acting self-incompatibility (LSI) in Bignoniaceae, despite the relatively few species investigated, and the occurrence of other kinds of breeding systems in this family. This study aimed to determine the breeding system in T. roseo-alba by means of controlled experimental pollination, histological analysis of post-pollination events, and studies of pistil longevity, in situ pollen tube growth and seed germination. Despite pollen tube penetration and fertilization of most ovules of selfed pistils, 100% of these pistils aborted uniformly between the fourth and sixth day after anthesis onset. Ovary and ovule growth, as well as initial endospermogenesis and proembryonal tube formation were verified in selfed pistils before abscission, but the progress of post-pollination development in these pistils was slower than in crossed pistils. The longevity of self-pollinated pistils was greater than in non-pollinated pistils. Seed germinability was 93%, and all seeds were monoembryonic. These results show that T. roseo-alba is a species with post-zygotic LSI, and with no polyembryony