Non-communicable diseases in the Western Area District, Sierra Leone, before and during the Ebola outbreak.

Setting: Twenty-seven peripheral health units, five secondary hospitals and one tertiary hospital, Western Area District, Sierra Leone. Objectives: To describe reporting systems, monthly attendances and facility-based patterns of six non-communicable diseases (NCDs) in the pre-Ebola and Ebola virus disease outbreak periods. Design: A cross-sectional study using programme data. Results: Reporting was 89% complete on the six selected NCDs pre-Ebola and 86% during the Ebola outbreak (P < 0.01). Overall, marked declining trends in NCDs were reported during the Ebola period, with a monthly mean of 342 cases pre-Ebola and 164 during the Ebola outbreak. The monthly mean number of cases per disease in the pre-Ebola and Ebola outbreak periods was respectively 228 vs. 85 for hypertension, 43 vs. 27 for cardiovascular diseases, 36 vs. 18 for diabetes and 25 vs. 29 for peptic ulcer disease; this last condition increased during the outbreak. There were higher proportions of NCDs among females during the Ebola outbreak compared with the pre-Ebola period. Except for peptic ulcer disease, the number of patients with NCDs declined by 25% in peripheral health units, 91% in the secondary hospitals and 70% in the tertiary hospital between the pre-Ebola and the Ebola outbreak periods. Conclusion: Comprehensive reporting of NCDs was suboptimal, and declined during the Ebola epidemic. There were decreases in reported attendances for NCDs between the pre-Ebola and the Ebola outbreak periods, which were even more marked in the hospitals. This study has important policy implications

Caractéristiques morphologiques et croissance de jeunes plants de Jatropha curcas L.

Une &#233;tude sur le d&#233;veloppement de jeunes plants de Jatropha curcas (L) provenant de boutures et de semis a &#233;t&#233; men&#233;e dans le Jardin botanique du D&#233;partement de Biologie v&#233;g&#233;tale de l&#8217;Universit&#233; Cheikh Anta Diop de Dakar en comparant diff&#233;rents param&#232;tres li&#233;s &#224; leur croissance et &#224; leur morphologie racinaire. Une culture de boutures et de semis en pots remplis de sol sablo-argileux et dispos&#233;s en bloc compl&#232;tement randomis&#233; puis un essai de semis direct sur une parcelle de 100 m2 du m&#234;me substrat ont &#233;t&#233; r&#233;alis&#233;s. Les r&#233;sultats ont montr&#233; que les segments de rameau proches de la tige d&#233;bourrent mieux (80%) que les segments apicaux (50%). Par ailleurs, aucune diff&#233;rence significative n&#8217;a &#233;t&#233; not&#233;e sur la germination entre les graines d&#233;cortiqu&#233;es (87%) et les graines non d&#233;cortiqu&#233;es (73,3%). Le rapport &#171; longueur racine / longueur tige &#187; est plus &#233;lev&#233; chez les boutures que chez les plants issus de semis. Les boutures ont &#233;mis des racines adventives dont l&#8217;une &#233;volue en pivot avec beaucoup de ramifications tandis que les autres racines disparaissent. Les semis en pot ont pr&#233;sent&#233; un enracinement dense avec plus de ramifications lat&#233;rales en forme d&#8217;&#233;chasse que les semis directs dont les ramifications ont, par ailleurs, &#233;t&#233; superficielles. Les mensurations effectu&#233;es sur la partie a&#233;rienne ont montr&#233; une croissance en diam&#232;tre chez les plants issus de semis direct et de semis en pot de 17,5 mm et de 13,08 mm chez les boutures.Mots cl&#233;s: Semis, bouture, tige, racin

Assessment of Vitamin A Status of Preschool Children in a Sub-Saharan African Setting: Comparative Advantage of Modified Relative-dose Response Test

A nationally-representative sample of 2,696 preschool children living in Congo was examined during August-September 2003 to determine the rates of vitamin A deficiency. Ninety clusters of 30 children, aged six months to six years, were selected, using a randomized two-level cluster-sampling method. Vitamin A deficiency was determined by assessing the prevalence of active xerophthalmia (nightblindness and/or Bitot spots) in the cross-over sample of 2,696 individuals. A semi-quantitative seven-day dietary questionnaire was concurrently applied to the mothers of children enrolled to estimate the latter's consumption of vitamin A-rich food. Vitamin A status was assessed by performing the modified relative dose-response test (MRDR) on dried blood spots (DBS) from a subsample of 207 children aged less than six years and the impression cytology with transfer (ICT) test on a subsample of 1,162 children. Of the children enrolled, 5.2% suffered from nightblindness, 8.0% had Bitot spots, and 2.5% had other vitamin A deficiency sequellae. Fifty-three percent of the ICT tests showed the presence of vitamin A deficiency. The biochemical MRDR test showed that the vitamin A status of 30% of the study children was critical. Twenty-seven of them had retinol levels of <10 μg/dL [mean±standard deviation (SD) 7.02±2.0 μg/dL], and 50% had retinol levels of 10-20 μg/dL (mean±SD 14.2±2.83 μg/dL). The poor health status and low rates of consumption of vitamin A-rich food are the main factors determining critical status. Vitamin A deficiency, reflecting poor nutrition and health, is a serious public-health issue among children aged less than six years in Congo

Effet du chlorure de sodium sur la germination de graines de Ricinus communis L.

Ricinus communis L (ricin) figure parmi les espèces du Programme biocarburant au  Sénégal. Sa culture sur des terrains incultes (terres salées) constitue une alternative pour éviter une concurrence sur les terres à vocation agricole. C’est pourquoi le taux de germination (TG) de sept accessions de graines de ricin a été étudié pendant 15 jours sous des concentrations croissantes de NaCl (0; 20; 50; 80; 100; 150 et 200 mM). Les résultats ont montré une large variabilité de tolérance au sel au stade de la germination. Les accessions 3 et 8 ont été très sensibles avec des TG réduits seulement à la concentration 20 mM NaCl. Les accessions 1, 6 et 7 n’ont pas toléré la concentration 50 mM. L’accession 5 n’a toléré que les teneurs &lt; 80 mM. L’accession 4 a germé dans les milieux ≤ 100 mM NaCl. Elle n’a manifesté une perte significative de TG qu’en présence de 150 mM NaCl. Elle a été la moins affectée par l’effet ralentisseur du sel sur la vitesse de germination. Ces résultats permettent de dire que le mode d’action de NaCl sur la germination est de nature osmotique et/ou toxique.Mots clés : Ricin, espèce, biocarburant, tolérance au sel, teneur en sel, Sénégal

Criblage in vitro des graines d’accessions locales de ricin (Ricinus communis L.) en conditions de stress salin

Le ricin (Ricinus communis L.) est une plante peu exigeante dont la culture offre d’énormes potentialités économiques pour les exploitants agricoles sénégalais. L’identification de génotypes performants avec des rendements acceptables en conditions de stress salin constitue une des solutions pour promouvoir cette culture sur des sols inaffectés aux cultures vivrières. Quatre accessions locales de ricin ont été cultivées durant un mois sur le milieu de Murashige et Skoog (MS) modifié et additionné de 0 ; 25 ; 50 ; 100 et 150 mM de chlorure de sodium (NaCl). Les réponses des accessions ont été évaluées en conditions in vitro sur la base de paramètres morphologiques, biochimiques et de survie des vitroplants. L’effet de la salinité sur ces paramètres mesurés a permis de classer les accessions en groupe tolérant (acc1), moyennement tolérant (acc7) et sensible (acc3 et acc4). La concentration 50 mM de NaCl a été identifiée comme un seuil de tolérance critique et discriminant permettant une classification des accessions de ricin selon leur sensibilité au NaCl. La biomasse aérienne est apparue comme un critère pertinent pour classer les accessions de ricin. Les mesures des teneurs en chlorophylle des feuilles des vitroplants ont permis d’aboutir à la même classification des groupes.Mots clés: criblage, Ricinus communis, salinité, SénégalEnglish Title:  In vitro screening of local accessions of castor bean seeds (Ricinus communis l.) Under conditions of salinity stressEnglish AbstractCastor bean (Ricinus communis L.) is an undemanding plant whose cultivation has enormous economic potential for Seneg alese farmers. The identification of efficient genotypes tolerant to salt stress condition could constitute a significant result to promote ricin culture specifically in margin soils. Four local accessions of castor were cultivated, using in vitro conditions for four weeks on MS modified medium supplemented with 0, 25, 50, 100, 150 mM NaCl. In vitro plants responses to salinity were recorded in terms of morphological and biochemical parameters and the rate of plant survival. The effect of different levels of salinity on these parameters was used to classify the accessions in tolerant group (acc1), moderately tolerant (acc7) and sensitive (acc3 and acc4). The concentration 50 mM of NaCl was identified as a discriminant level for castor accessions classification and a critical level of tolerance. The in vitro plants shoot biomass, appeared a relevant parameter to be used for classification of castor accessions under in vitro conditions. The content of leaf chlorophyll for in vitro plants confirmed the classification of Castor bean accessions obtained with morphological parameters according to their sensitivity to the salinity.Keywords: screening, Ricinus communis, salinity, Senega

Biological nitrogen fixation in Crotalaria species estimated using the 15N isotope dilution method

A greenhouse experiment was conducted to measure nitrogen fixation in three Crotalaria species : C. ochroleuca, C. perrottetii and C. retusa growing in Senegal by using 15N direct isotope dilution technique. Two non-fixing plants, Senna obtusifolia and Senna occidentalis served as reference plants. The amount of nitrogen fixed two months after planting was obtained using the average of the two reference plants. The atom % 15N excess in the Crotalaria species was significantly lower than that of the reference plants, indicating that significant nitrogen fixation occurred in the three plants. Significant differences were observed between the Crotalaria species; C. ochroleuca yielded more dry matter weight and total nitrogen than did C. perrottetti and C. retusa. The % nitrogen derived from atmosphere (%Ndfa) in leaves and stems was also higher in C. ochroleuca. There was no significant difference in %Ndfa in the whole plant between the three Crotalaria species (47% to 53%). In contrast, interspecific variability was observed based on the %Ndfa. C. ochroleuca significantly exhibited the higher amount of total nitrogen fixed, equivalent to 83 kg of nitrogen fixed per hectare. Based on these data, it was concluded that C. ochroleuca could be used in multiple cropping systems in Senegal for making more nitrogen available to other plants. (African Journal of Biotechnology: 2002 1(1): 17-22

Pharyngeal carriage of Neisseria species in the African meningitis belt.

OBJECTIVES: Neisseria meningitidis, together with the non-pathogenic Neisseria species (NPNs), are members of the complex microbiota of the human pharynx. This paper investigates the influence of NPNs on the epidemiology of meningococcal infection. METHODS: Neisseria isolates were collected during 18 surveys conducted in six countries in the African meningitis belt between 2010 and 2012 and characterized at the rplF locus to determine species and at the variable region of the fetA antigen gene. Prevalence and risk factors for carriage were analyzed. RESULTS: A total of 4694 isolates of Neisseria were obtained from 46,034 pharyngeal swabs, a carriage prevalence of 10.2% (95% CI, 9.8-10.5). Five Neisseria species were identified, the most prevalent NPN being Neisseria lactamica. Six hundred and thirty-six combinations of rplF/fetA_VR alleles were identified, each defined as a Neisseria strain type. There was an inverse relationship between carriage of N. meningitidis and of NPNs by age group, gender and season, whereas carriage of both N. meningitidis and NPNs was negatively associated with a recent history of meningococcal vaccination. CONCLUSION: Variations in the prevalence of NPNs by time, place and genetic type may contribute to the particular epidemiology of meningococcal disease in the African meningitis belt

Assessment of vitamin A status of preschool children in a sub-Saharan African setting: Comparative advantage of modified relative-dose response test

A nationally-representative sample of 2, 696 preschool children living in Congo was examined during Au-gust-September 2003 to determine the rates of vitamin A deficiency. Ninety clusters of 30 children, aged six months to six years, were selected, using a randomized two-level cluster-sampling method. Vitamin A deficiency was determined by assessing the prevalence of active xerophthalmia (nightblindness and/or Bitot spots) in the cross-over sample of 2, 696 individuals. A semi-quantitative seven-day dietary question-naire was concurrently applied to the mothers of children enrolled to estimate the latter\u2032s consumption of vitamin A-rich food. Vitamin A status was assessed by performing the modified relative dose-response test (MRDR) on dried blood spots (DBS) from a subsample of 207 children aged less than six years and the im-pression cytology with transfer (ICT) test on a subsample of 1, 162 children. Of the children enrolled, 5. 2% suffered from nightblindness, 8. 0% had Bitot spots, and 2. 5% had other vitamin A deficiency sequellae. Fifty-three percent of the ICT tests showed the presence of vitamin A deficiency. The biochemical MRDR test showed that the vitamin A status of 30% of the study children was critical. Twenty-seven of them had retinol levels of &lt; 10 \u3bcg/dL [mean\ub1standard deviation (SD) 7. 02\ub1 2. 0 \u3bcg/dL], and 50% had retinol levels of 10- 20 \u3bcg/dL (mean\ub1SD 14. 2\ub1 2. 83 \u3bcg/dL). The poor health status and low rates of consumption of vitamin A-rich food are the main factors determining critical status. Vitamin A deficiency, reflecting poor nutrition and health, is a serious public-health issue among children aged less than six years in Congo