Quantum pump effect in one-dimensional systems of Dirac fermions

We investigate the behavior of the directed current in one-dimensional systems of Dirac fermions driven by local periodic potentials in the forward as well in backscattering channels. We treat the problem with Keldysh non-equilibrium Green's function formalism. We present the exact solution for the case of an infinite wire and show that in this case the dc current vanishes identically. We also investigate a confined system consistent in an annular arrangement coupled to a particle reservoir. We present a perturbative treatment that allows for the analytical expressions of the dc current in the lowest order of the amplitudes of the potential. We also present results obtained from the exact numerical solution of the problem.Comment: 8 pages, 5 figure

Non-perturbative approach to backscattering off a dynamical impurity in 1D Fermi systems

We investigate the problem of backscattering off a time-dependent impurity in a one-dimensional electron gas. By combining the Schwinger-Keldysh method with an adiabatic approximation in order to deal with the corresponding out of equilibrium Dirac equation, we compute the total energy density (TED) of the system. We show how the free fermion TED is distorted by the backscattering amplitude and the geometry of the impurity.Comment: 5 pages, 2 figures, RevTex4. Appendix and some text added. Results and conclusions did not change. Version accepted for publication in Phys. Rev.

Transient effects in the backscattered current of a Luttinger liquid

We study the backscattered current in a Luttinger liquid in the presence of a point like weak impurity switched on at finite time, taking into account finite-temperature effects. We show how the well-known results for a static impurity are distorted. We derive a dimensionless parameter $\tau_{R}$ as function of the electron-electron interaction and the temperature, such that for $\tau_{R} 1$) the switching process is relevant (irrelevant). Our results suggest the possibility of determining the value of the Luttinger parameter $K$ through time measurements in transport experiments at fixed voltage.Comment: 8 pages, 3 figures. To appear in Physical Review

Pumping current of a Luttinger liquid with finite length

We study transport properties in a Tomonaga-Luttinger liquid in the presence of two time-dependent point like weak impurities, taking into account finite-length effects. By employing analytical methods and performing a perturbation theory, we compute the backscattering pumping current (I_bs) in different regimes which can be established in relation to the oscillatory frequency of the impurities and to the frequency related to the length and the renormalized velocity (by the electron-electron interactions) of the charge density modes. We investigate the role played by the spatial position of the impurity potentials. We also show how the previous infinite length results for I_bs are modified by the finite size of the system.Comment: 9 pages, 7 figure

Oligomeric Status and Nucleotide Binding Properties of the Plastid ATP/ADP Transporter 1: Toward a Molecular Understanding of the Transport Mechanism

Background: Chloroplast ATP/ADP transporters are essential to energy homeostasis in plant cells. However, their molecular mechanism remains poorly understood, primarily due to the difficulty of producing and purifying functional recombinant forms of these transporters. Methodology/Principal Findings: In this work, we describe an expression and purification protocol providing good yields and efficient solubilization of NTT1 protein from Arabidopsis thaliana. By biochemical and biophysical analyses, we identified the best detergent for solubilization and purification of functional proteins, LAPAO. Purified NTT1 was found to accumulate as two independent pools of well folded, stable monomers and dimers. ATP and ADP binding properties were determined, and Pi, a co-substrate of ADP, was confirmed to be essential for nucleotide steady-state transport. Nucleotide binding studies and analysis of NTT1 mutants lead us to suggest the existence of two distinct and probably inter-dependent binding sites. Finally, fusion and deletion experiments demonstrated that the C-terminus of NTT1 is not essential for multimerization, but probably plays a regulatory role, controlling the nucleotide exchange rate. Conclusions/Significance: Taken together, these data provide a comprehensive molecular characterization of a chloroplas

Density Contrast Sedimentation Velocity for the Determination of Protein Partial-Specific Volumes

The partial-specific volume of proteins is an important thermodynamic parameter required for the interpretation of data in several biophysical disciplines. Building on recent advances in the use of density variation sedimentation velocity analytical ultracentrifugation for the determination of macromolecular partial-specific volumes, we have explored a direct global modeling approach describing the sedimentation boundaries in different solvents with a joint differential sedimentation coefficient distribution. This takes full advantage of the influence of different macromolecular buoyancy on both the spread and the velocity of the sedimentation boundary. It should lend itself well to the study of interacting macromolecules and/or heterogeneous samples in microgram quantities. Model applications to three protein samples studied in either H2O, or isotopically enriched H218O mixtures, indicate that partial-specific volumes can be determined with a statistical precision of better than 0.5%, provided signal/noise ratios of 50–100 can be achieved in the measurement of the macromolecular sedimentation velocity profiles. The approach is implemented in the global modeling software SEDPHAT

Hydration effects on the structural properties and haem-haem interaction in haemoglobin

Direct and simultaneous measurements of hydration water content and protein conformation have been performed using quartz crystal microbalance and visible absorption spectroscopy. Equilibrium and kinetics of methaemoglobin/haemichrome transition induced by the alteration of the degree of hydration was investigated in thin films exposed to controlled humidity. The kinetics experiment show that the conversion of species achieve the equilibrium more rapidly that the amount of sorbed water by the protein. The transition shows a sigmoid behaviour and suggest cooperative phenomena manifested by haem-haem interaction. The water hydration network contributing to the haem haem interaction advise that water acts as allosteric effectors for the conversion between species. Irreversible changes produced by complete drying are clearly shown

New Antioxidant Active Packaging Films Based on Yeast Cell Wall and Naphtho-γ-Pyrone Extract.

International audienceThe main objective of this work is the development of new active films based on yeast cell wall obtained by high-pressure homogenization (YCW-H) supplemented with naphtho-γ-pyrone (CL-NGP) extract, which is a bioactive compound produced by Aspergillus tubingensis G131 with great antioxidant potential. A complete characterization of the functional properties of the bioactive films, such as their structural, colour, thermal, mechanical, hydration and water vapour transport, was carried out to evaluate the influence of the addition of the antioxidant compounds. Likewise, the antioxidant capacity of the developed materials and the specific migration of NGPs in food simulants were evaluated. The results showed that CL-NGP extract possessed an important antioxidant activity, which was maintained after incorporation in YCW-H films. The addition of 2 and 5% CL-NGPs decreased the hydration of films and consequently improved the water vapour barrier properties. It was observed that CL-NGPs migrate in fatty food simulants and retain their antioxidant capacity in the simulant. The results obtained in this work showed that bioactive films based on yeast cell walls with the addition of CL-NGPs have the potential to be used as packaging material in systems of interest in the food industry