Distribution of non-LEE-encoded type 3 secretion system dependent effectors in enteropathogenic Escherichia coli

Enteropathogenic Escherichia coli (EPEC) are important human gastroenteritis agents. The prevalence of six non-LEE genes encoding type 3 translocated effectors was investigated. The nleC, cif and nleB genes were more prevalent in typical than in atypical EPEC, although a higher diversity of genes combinations was observed in atypical EPEC.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Federal de São Paulo (UNIFESP) Departamento de Microbiologia, Imunologia e ParasitologiaUniversidade Estadual Paulista Julio de Mesquita Filho Instituto de Biociências Departamento de Microbiologia e ImunologiaUNIFESP, Depto. de Microbiologia, Imunologia e ParasitologiaSciEL

Characterization of atypical enteropathogenic Escherichia coli strains that express typical localized adherence in HeLa cells in the absence of the bundle-forming pilus

The characterization of nine atypical enteropathogenic Escherichia coli strains expressing localized adherence in HeLa cells in the absence of the bundle-forming pilus revealed a diversity of serotypes, plasmids, and virulence genes. Although the strains lacked known E. coli adhesin genes, the identification of new adhesins could contribute to the characterization of similar enteropathogenic E. coli isolates.Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, BrazilInst Butanan, Lab Biol Cellular, São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, BrazilWeb of Scienc

Prevalence and Characteristics of the O122 Pathogenicity Island in Typical and Atypical Enteropathogenic Escherichia coli Strains

The presence of the pathogenicity island (PAI) O122 genes, efa1 (lifA), sen, pagC, nleB, and nleE, in typical and atypical enteropathogenic Escherichia coli (EPEC) strains was investigated. the simultaneous occurrence of all genes was statistically associated with diarrhea due to atypical EPEC. Detection of the complete PAI O122 could aid in the identification of potential pathogenic strains of atypical EPEC.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal de São Paulo, Dept Microbiol, São Paulo, BrazilSecao Bacteriol Inst Adolfo Lutz, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Microbiol, São Paulo, BrazilFAPESP: 08/53812-4Web of Scienc

Characterization of Atypical Enteropathogenic Escherichia coli Strains That Express Typical Localized Adherence in HeLa Cells in the Absence of the Bundle-Forming Pilus

The characterization of nine atypical enteropathogenic Escherichia coli strains expressing localized adherence in HeLa cells in the absence of the bundle-forming pilus revealed a diversity of serotypes, plasmids, and virulence genes. Although the strains lacked known E. coli adhesin genes, the identification of new adhesins could contribute to the characterization of similar enteropathogenic E. coli isolates