41 research outputs found
Monitoring of patogen parasites in waste waters of agricultural industries
Las aguas residuales son potenciales
portadoras de enteroparásitos patógenos
para el hombre cuya dosis infectiva puede
ser mínima y prolongado el tiempo de
sobrevivencia de huevos y quistes en el
ambiente. La reglamentación limita la presencia
de huevos de helmintos y, hasta el
año 2000, no proponía una técnica para su
detección. Este trabajo se realizó para caracterizar
parasitológicamente los efluentes
procedentes de agroindustrias.
La capacitación en la identificación microscópica
demandó la confección de fichas
que describen las características de huevos
de helmintos. Se efectuaron ensayos con
muestras de bodegas y aceiteras en los puntos
detectados de contaminación. Los métodos
fueron adaptados a las particularidades
de los efluentes.
Los resultados no señalaron presencia
de helmintos; no obstante, se hallaron
Cyclospora e Isospora, protozoarios que, por
su alto poder infectivo, también constituyen
un riesgo para el hombre. Sin embargo, no
están contemplados por la legislación. Respecto
a helmintos, es necesario un estudio
comparativo de las técnicas de detección
para efluentes agroindustriales, con el fin de
establecer las que optimicen su recuperación.
En cuanto a otras especies es preciso
continuar investigando su presencia para
evaluar la conveniencia de incorporarlas a
la reglamentación.Wastewaters are potential human's
pathogenic parasite carriers which could be
infective in small quantities. Besides, the
eggs and cysts survival time could be
prolonged. Regulation, restricts helminths
eggs presence and, until 2000, did not
propose a detection technique. This research
aims to the parasitological characterization
of waste water from agrarian industries.
Training about microscopical identification
led us to write a helminths eggs
characteristics description. Since the
detection of pollution points, essays were
made with samples from wine vault and olive
oil factories. Techniques were adapted,
according to wastewater peculiarities.
The results did not show helminths
presence, but we found Cyclospora and
Isospora. They are protozoa whose high
infective power makes them a human health
risk. However, they are not included in
current legislation. With respect to helminths,
we concluded that it is important to make a
detection techniques comparative study for
wastewater in order to establish which one
is the best for helminths recovery.
Since other species identification, it is
necessary to investigate their presence in
order to evaluate the convenience of
incorporating them to water regulation.Fil: Enriquez, Verónica L..
Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Departamento de Biomatemática y FisicoquímicaFil: Soria, Adriana E..
Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Departamento de Biomatemática y FisicoquímicaFil: Salomón, María C..
Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Departamento de Patologí
Vitamin D receptor expression is associated with improved overall survival in human glioblastoma multiforme
Vitamin D and its analogs have been shown to display anti-proliferative effects in a wide variety of cancer types including glioblastoma multiforme (GBM). These anticancer effects are mediated by its active metabolite, 1α, 25-dihydroxyvitamin D3 (calcitriol) acting mainly through vitamin D receptor (VDR) signaling. In addition to its involvement in calcitriol action, VDR has also been demonstrated to be useful as a prognostic factor for some types of cancer. However, to our knowledge, there are no studies evaluating the expression of VDR protein and its association with outcome in gliomas. Therefore, we investigated VDR expression by using immunohistochemical analysis in human glioma tissue microarrays, and analyzed the association between VDR expression and clinico-pathological parameters. We further investigated the effects of genetic and pharmacologic modulation of VDR on survival and migration of glioma cell lines. Our data demonstrate that VDR is increased in tumor tissues when compared with VDR in non-malignant brains, and that VDR expression is associated with an improved outcome in patients with GBM. We also show that both genetic and pharmacologic modulation of VDR modulates GBM cellular migration and survival and that VDR is necessary for calcitriol-mediated effects on migration. Altogether these results provide some limited evidence supporting a role for VDR in glioma progression.Fil: Salomón, Débora Gisele. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Bahia Blanca. Instituto de Investigaciones Bioquimicas Bahia Blanca (i); ArgentinaFil: Fermento, María Eugenia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Bahia Blanca. Instituto de Investigaciones Bioquimicas Bahia Blanca (i); ArgentinaFil: Gandini, Norberto Ariel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Bahia Blanca. Instituto de Investigaciones Bioquimicas Bahia Blanca (i); ArgentinaFil: Ferronato, María Julia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Bahia Blanca. Instituto de Investigaciones Bioquimicas Bahia Blanca (i); ArgentinaFil: Arevalo, Julian. Hospital Municipal General de Agudos Doctor José Penna; ArgentinaFil: Blasco, J.. Hospital Int. Gral. de Agudos Dr. Jose Penna. Servicio de Patologia; ArgentinaFil: Andrés, Nancy Carolina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Bahia Blanca. Instituto de Investigaciones Bioquimicas Bahia Blanca (i); ArgentinaFil: Zenklusen, Jean C.. National Institutes of Health. National Cancer Institute; Estados UnidosFil: Curino, Alejandro Carlos. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Bahia Blanca. Instituto de Investigaciones Bioquimicas Bahia Blanca (i); ArgentinaFil: Facchinetti, Maria Marta. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Bahia Blanca. Instituto de Investigaciones Bioquimicas Bahia Blanca (i); Argentin
Lutzomyia longipalpis in Uruguay: the first report and the potential of visceral leishmaniasis transmission
Optimization of DNA Extraction from Individual Sand Flies for PCR Amplification
Numerous protocols have been published for extracting DNA from phlebotomines.
Nevertheless, their small size is generally an issue in terms of yield, efficiency, and purity, for large-scale individual sand fly DNA extractions when using traditional methods. Even though this can be circumvented with commercial kits, these are generally cost-prohibitive for developing countries.
We encountered these limitations when analyzing field-collected Lutzomyia spp. by polymerase chain reaction (PCR) and, for this reason, we evaluated various modifications on a previously published protocol, the most significant of which was a different lysis buffer that contained Ca2+ (buffer TESCa).
This ion protects proteinase K against autolysis, increases its thermal stability, and could have a regulatory function for its substrate-binding site. Individual sand fly DNA extraction success was confirmed by amplification reactions using internal control primers that amplify a fragment of the cacophony gene. To the best of our knowledge, this is the first time a lysis buffer containing Ca2+ has been reported for the extraction of DNA from sand flies.Centro Regional de Estudios Genómico
The isolation and molecular characterization of Leishmania spp. from patients with American tegumentary leishmaniasis in northwest Argentina
Spatial and temporal changes in Lutzomyia longipalpis abundance, a Leishmania infantum vector in an urban area in northeastern Argentina
ESR1 gene promoter region methylation in free circulating DNA and its correlation with estrogen receptor protein expression in tumor tissue in breast cancer patients
[Background]
Tumor expression of estrogen receptor (ER) is an important marker of prognosis, and is predictive of response to endocrine therapy in breast cancer. Several studies have observed that epigenetic events, such methylation of cytosines and deacetylation of histones, are involved in the complex mechanisms that regulate promoter transcription. However, the exact interplay of these factors in transcription activity is not well understood. In this study, we explored the relationship between ER expression status in tumor tissue samples and the methylation of the 5′ CpG promoter region of the estrogen receptor gene (ESR1) isolated from free circulating DNA (fcDNA) in plasma samples from breast cancer patients.
[Methods]
Patients (n = 110) with non-metastatic breast cancer had analyses performed of ER expression (luminal phenotype in tumor tissue, by immunohistochemistry method), and the ESR1-DNA methylation status (fcDNA in plasma, by quantitative methylation specific PCR technique).
[Results]
Our results showed a significant association between presence of methylated ESR1 in patients with breast cancer and ER negative status in the tumor tissue (p = 0.0179). There was a trend towards a higher probability of ESR1-methylation in those phenotypes with poor prognosis i.e. 80% of triple negative patients, 60% of HER2 patients, compared to 28% and 5.9% of patients with better prognosis such as luminal A and luminal B, respectively.
[Conclusion]
Silencing, by methylation, of the promoter region of the ESR1 affects the expression of the estrogen receptor protein in tumors of breast cancer patients; high methylation of ESR1-DNA is associated with estrogen receptor negative status which, in turn, may be implicated in the patient’s resistance to hormonal treatment in breast cancer. As such, epigenetic markers in plasma may be of interest as new targets for anticancer therapy, especially with respect to endocrine treatment.The study was funded, in part, by a grant from the Ministerio de Educación y Ciencia (CICYT: SAF 2004–00889)