Fast-Light in a Photorefractive Crystal for Gravitational Wave Detection

We demonstrate superluminal light propagation using two frequency multiplexed pump beams to produce a gain doublet in a photorefractive crystal of Ce:BaTiO3. The two gain lines are obtained by two-wave mixing between a probe field and two individual pump fields. The angular frequencies of the pumps are symmetrically tuned from the frequency of the probe. The frequency difference between the pumps corresponds to the separation of the two gain lines; as it increases, the crystal gradually converts from normal dispersion without detuning to an anomalously dispersive medium. The time advance is measured as 0.28 sec for a pulse propagating through a medium with a 2Hz gain separation, compared to the same pulse propagating through empty space. We also demonstrate directly anomalous dispersion profile using a modfied experimental configuration. Finally, we discuss how anomalous dispersion produced this way in a faster photorefractive crystal (such as SPS: Sn2P2S6) could be employed to enhance the sensitivity-bandwidth product of a LIGO type gravitational wave detector augmented by a White Light Cavity.Comment: 14 pages, 5 figure

Plasmon dispersion in semimetallic armchair graphene nanoribbons

The dispersion relations for plasmons in intrinsic and extrinsic semimetallic armchair graphene nanoribbons (acGNR) are calculated in the random phase approximation using the orthogonal p_z-orbital tight binding method. Our model predicts new plasmons for acGNR of odd atomic widths N=5,11,17,... Our model further predicts plasmons in acGNR of even atomic width N=2,8,14,... related to those found using a Dirac continuum model, but with different quantitative dispersion characteristics. We find that the dispersion of all plasmons in semimetallic acGNR depends strongly on the localization of the p_z electronic wavefunctions. We also find that overlap integrals for acGNR behave in a more complex way than predicted by the Dirac continuum model, suggesting that these plasmons will experience a small damping for all q not equal to 0. Plasmons in extrinsic semimetallic acGNR with the chemical potential in the lowest (highest) conduction (valence) band are found to have dispersion characteristics nearly identical to their intrinsic counterparts, with negligible differencs in dispersion arising from the slight differences in overlap integrals for the interband and intraband transitions.Comment: 8 pages, 9 figure

Photon lifetime in a cavity containing a slow-light medium

We investigate experimentally the lifetime of the photons in a cavity containing a medium exhibiting strong positive dispersion. This intracavity positive dispersion is provided by a metastable helium gas at room temperature in the electromagnetically induced transparency (EIT) regime, in which light propagates at a group velocity of the order of 10000 m/s. The results definitely prove that the lifetime of the cavity photons is governed by the group velocity of light in the cavity, and not its phase velocity.Comment: Accepted for publication in Optics Letter

Synthetic spike-in standards for RNA-seq experiments

High-throughput sequencing of cDNA (RNA-seq) is a widely deployed transcriptome profiling and annotation technique, but questions about the performance of different protocols and platforms remain. We used a newly developed pool of 96 synthetic RNAs with various lengths, and GC content covering a 2(20) concentration range as spike-in controls to measure sensitivity, accuracy, and biases in RNA-seq experiments as well as to derive standard curves for quantifying the abundance of transcripts. We observed linearity between read density and RNA input over the entire detection range and excellent agreement between replicates, but we observed significantly larger imprecision than expected under pure Poisson sampling errors. We use the control RNAs to directly measure reproducible protocol-dependent biases due to GC content and transcript length as well as stereotypic heterogeneity in coverage across transcripts correlated with position relative to RNA termini and priming sequence bias. These effects lead to biased quantification for short transcripts and individual exons, which is a serious problem for measurements of isoform abundances, but that can partially be corrected using appropriate models of bias. By using the control RNAs, we derive limits for the discovery and detection of rare transcripts in RNA-seq experiments. By using data collected as part of the model organism and human Encyclopedia of DNA Elements projects (ENCODE and modENCODE), we demonstrate that external RNA controls are a useful resource for evaluating sensitivity and accuracy of RNA-seq experiments for transcriptome discovery and quantification. These quality metrics facilitate comparable analysis across different samples, protocols, and platforms

Smoking-mediated up-regulation of GAD67 expression in the human airway epithelium

<p>Abstract</p> <p>Background</p> <p>The production of gamma-amino butyric acid (GABA) is dependent on glutamate decarboxylases (GAD65 and GAD67), the enzymes that catalyze the decarboxylation of glutamate to GABA. Based on studies suggesting a role of the airway epithelial GABAergic system in asthma-related mucus overproduction, we hypothesized that cigarette smoking, another disorder associated with increased mucus production, may modulate GABAergic system-related gene expression levels in the airway epithelium.</p> <p>Methods</p> <p>We assessed expression of the GABAergic system in human airway epithelium obtained using bronchoscopy to sample the epithelium and microarrays to evaluate gene expression. RT-PCR was used to confirm gene expression of GABAergic system gene in large and small airway epithelium from heathy nonsmokers and healthy smokers. The differences in the GABAergic system gene was further confirmed by TaqMan, immunohistochemistry and Western analysis.</p> <p>Results</p> <p>The data demonstrate there is a complete GABAergic system expressed in the large and small human airway epithelium, including glutamate decarboxylase, GABA receptors, transporters and catabolism enzymes. Interestingly, of the entire GABAergic system, smoking modified only the expression of GAD67, with marked up-regulation of GAD67 gene expression in both large (4.1-fold increase, p < 0.01) and small airway epithelium of healthy smokers (6.3-fold increase, p < 0.01). At the protein level, Western analysis confirmed the increased expression of GAD67 in airway epithelium of healthy smokers compared to healthy nonsmokers (p < 0.05). There was a significant positive correlation between GAD67 and MUC5AC gene expression in both large and small airway epithelium (p < 0.01), implying a link between GAD67 and mucin overproduction in association with smoking.</p> <p>Conclusions</p> <p>In the context that GAD67 is the rate limiting enzyme in GABA synthesis, the correlation of GAD67 gene expression with MUC5AC expressions suggests that the up-regulation of airway epithelium expression of GAD67 may contribute to the increase in mucus production observed in association with cigarette smoking.</p> <p>Trial registration</p> <p>NCT00224198; NCT00224185</p

Mercerization effect on morphology and tensile properties of roselle fibre

Natural fibres are preferred compared to synthetic fibres because of several advantages such as biodegradable, lightweight, low cost and good mechanical properties. Roselle is one of the plants found to be suitable to be used to produce natural fibres. Although natural fiber reinforced composites are becoming widely used, several weaknesses such as lack of good interfacial adhesion, low melting point and poor resistance to moisture absorption are harmful to its further acceptance. Chemical treatment is a method that can improve the interfacial bonding, stop water absorption, clean the fibre and increase surface roughness. In this study, roselle fibres were immersed in Sodium hydroxide (NaOH) with 3 different concentration (3, 6, and 9%). The results before and after treatment were compared. Scanning electron microscope was used to examine the surface morphology. Tensile properties of roselle fibre were performed to study the tensile properties. Results shows that the higher concentration of NaOH will increase the surface roughness and have higher ability to clean the fibre. For tensile properties, 6% of NaOH give the highest tensile strength. It can be concluded that, 6% of NaOH is the most suitable concentration to clean roselle fibre and while maintaining good tensile properties

The Human Airway Epithelial Basal Cell Transcriptome

The human airway epithelium consists of 4 major cell types: ciliated, secretory, columnar and basal cells. During natural turnover and in response to injury, the airway basal cells function as stem/progenitor cells for the other airway cell types. The objective of this study is to better understand human airway epithelial basal cell biology by defining the gene expression signature of this cell population.. The basal cell signature displayed overlap with genes expressed in basal-like cells from other human tissues and with that of murine airway basal cells. Consistent with self-modulation as well as signaling to other airway cell types, the human airway basal cell signature was characterized by genes encoding extracellular matrix components, growth factors and growth factor receptors, including genes related to the EGF and VEGF pathways. Interestingly, while the basal cell signature overlaps that of basal-like cells of other organs, the human airway basal cell signature has features not previously associated with this cell type, including a unique pattern of genes encoding extracellular matrix components, G protein-coupled receptors, neuroactive ligands and receptors, and ion channels.The human airway epithelial basal cell signature identified in the present study provides novel insights into the molecular phenotype and biology of the stem/progenitor cells of the human airway epithelium