33 research outputs found
Evaluation of skin irritation in rats using simultaneous laser Doppler flowmetry and oxygenation monitoring
Β© 2016, Springer Science+Business Media New York.The possibility of assessment of skin irritation in rats with the use of simultaneous laser Doppler flowmetry (LDF) and oxygenation monitoring was investigated. The study was designed according to ISO 10993-10 using formaldehyde as a model irritant. 4-Hour application of 1Β % formaldehyde induced significant local increase in skin microcirculation for the first 2Β days by a factor of up to 2.4, whereas tissue oxygenation increased in much lower extent. The microcirculation was significantly inhibited by 10Β % formaldehyde following treatment and then it gradually increased for 24 and 48Β h of recovery period. The results correlated with visual evaluation of skin edema and erythema in treated zones. LDF/oxygenation signals provide early information on skin irritation and damage in rat model. The technique is valuable for preclinical/toxicological studies of chemical substances and medical materials
Geometrical structures of the gem-dichlorocyclopropyl aryl selenides
1. The para-substituted gem-dichloropropyl aryl selenides have been synthesized, for the first time, and studied through their13C NMR spectra and their dipole moments and Kerr constants. 2. These compounds exist as gauche conformers with syn and anti orientation of the aromatic ring with respect to the geminal C-Cl bonds. 3. Determination has been made of the dipole moment of the {Mathematical expression} bond. Β© 1980 Plenum Publishing Corporation
Pulseless disease
The article discusses a rare form of large vessel vasculitis β Takayasu arteritis, based on the literature data; the questions of epidemiological, the sequence of diagnostic procedures and treatment tactics are reflectedΠ ΡΡΠ°ΡΡΠ΅ ΡΠ°ΡΡΠΌΠΎΡΡΠ΅Π½Π° ΡΠ΅Π΄ΠΊΠ°Ρ ΡΠΎΡΠΌΠ° Π²Π°ΡΠΊΡΠ»ΠΈΡΠ° ΠΊΡΡΠΏΠ½ΡΡ
Π°ΡΡΠ΅ΡΠΈΠΉ - ΠΡΡΠ΅ΡΠΈΠΈΡ Π’Π°ΠΊΠ°ΡΡΡ, Π½Π° ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΠΈ Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΠ½ΡΡ
Π΄Π°Π½Π½ΡΡ
ΠΎΡΡΠ°ΠΆΠ΅Π½Ρ Π²ΠΎΠΏΡΠΎΡΡ ΡΠΏΠΈΠ΄Π΅ΠΌΠΈΠΎΠ»ΠΎΠ³ΠΈΠΈ, ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΡ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΡ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΡΠΎΡΠ΅Π΄ΡΡ ΠΈ ΡΠ°ΠΊΡΠΈΠΊΠΈ Π»Π΅ΡΠ΅Π½ΠΈ
The attitude of USMU students to vaccination against COVID-19
The aim of the study β to determine the attitude of medical students to vaccination against COVID-19 (motivation, possible problems, post-vaccination reactions).Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ β ΠΎΠΏΡΠ΅Π΄Π΅Π»ΠΈΡΡ ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠ΅ ΡΡΡΠ΄Π΅Π½ΡΠΎΠ²-ΠΌΠ΅Π΄ΠΈΠΊΠΎΠ² ΠΊ Π²Π°ΠΊΡΠΈΠ½ΠΎΠΏΡΠΎΡΠΈΠ»Π°ΠΊΡΠΈΠΊΠ΅ COVID-19 (ΠΌΠΎΡΠΈΠ²Π°ΡΠΈΡ, Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΡΠ΅ ΠΏΡΠΎΠ±Π»Π΅ΠΌΡ, ΠΏΠΎΡΡΠ²Π°ΠΊΡΠΈΠ½Π°Π»ΡΠ½ΡΠ΅ ΡΠ΅Π°ΠΊΡΠΈΠΈ)
Epidemiology of bloodstream infections in the burn icu
The study included data on 80 adult patients hospitalized in the ICU of the Yekaterinburg Burn Center in 2018 - 2019, who underwent at least one bacteriological blood test. In a small observational study, bloodstream infection did not lead to an increase in mortality, but is an important factor in increasing the incidence (length of hospitalization and ICU). In 70,5% of cases, the source of bloodstream infections are P. aeruginosa and S. aureus. The duration of standing of the CVC is one of the most important criteria for the development of bloodstream infectionsΠ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Π²ΠΊΠ»ΡΡΠ΅Π½Ρ Π΄Π°Π½Π½ΡΠ΅ ΠΎ 80 Π²Π·ΡΠΎΡΠ»ΡΡ
ΠΏΠ°ΡΠΈΠ΅Π½ΡΠ°Ρ
, Π³ΠΎΡΠΏΠΈΡΠ°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
Π² ΠΠ ΠΠ’ ΠΎΠΆΠΎΠ³ΠΎΠ²ΠΎΠ³ΠΎ ΡΠ΅Π½ΡΡΠ° ΠΠΊΠ°ΡΠ΅ΡΠΈΠ½Π±ΡΡΠ³Π° Π² 2018 β 2019 Π³Π³, ΠΊΠΎΡΠΎΡΡΠΌ Π±ΡΠ»ΠΎ ΡΠ΄Π΅Π»Π°Π½ΠΎ ΠΏΠΎ ΠΊΡΠ°ΠΉΠ½Π΅ΠΉ ΠΌΠ΅ΡΠ΅ ΠΎΠ΄Π½ΠΎ Π±Π°ΠΊΡΠ΅ΡΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΊΡΠΎΠ²ΠΈ. Π Π½Π΅Π±ΠΎΠ»ΡΡΠΎΠΌ ΠΎΠ±ΡΠ΅ΡΠ²Π°ΡΠΈΠΎΠ½Π½ΠΎΠΌ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΈ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΡ ΠΊΡΠΎΠ²ΠΎΡΠΎΠΊΠ° Π½Π΅ ΠΏΡΠΈΠ²Π΅Π»Π° ΠΊ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΡ Π»Π΅ΡΠ°Π»ΡΠ½ΠΎΡΡΠΈ, Π½ΠΎ ΡΠ²Π»ΡΠ΅ΡΡΡ Π²Π°ΠΆΠ½ΡΠΌ ΡΠ°ΠΊΡΠΎΡΠΎΠΌ ΡΠ²Π΅Π»ΠΈΡΠΈΠ²Π°ΡΡΠ΅ΠΌ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π΅ΠΌΠΎΡΡΡ (Π΄Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΡΡΡ Π³ΠΎΡΠΏΠΈΡΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ Π² ΡΡΠ°ΡΠΈΠΎΠ½Π°ΡΠ΅ ΠΈ ΠΠ ΠΠ’). Π 70,5% ΡΠ»ΡΡΠ°Π΅Π² ΠΈΡΡΠΎΡΠ½ΠΈΠΊΠΎΠΌ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΉ ΠΊΡΠΎΠ²ΠΎΡΠΎΠΊΠ° ΡΠ²Π»ΡΡΡΡΡ P. aeruginosa ΠΈ S. aureus. ΠΠ»ΠΈΡΠ΅Π»ΡΠ½ΠΎΡΡΡ ΡΡΠΎΡΠ½ΠΈΡ Π¦ΠΠ ΠΎΠ΄ΠΈΠ½ ΠΈΠ· Π²Π°ΠΆΠ½Π΅ΠΉΡΠΈΡ
ΠΊΡΠΈΡΠ΅ΡΠΈΠ΅Π², ΠΎΠ±ΡΡΠ»Π°Π²Π»ΠΈΠ²Π°ΡΡΠΈΡ
ΡΠ°Π·Π²ΠΈΡΠΈΠ΅ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΉ ΠΊΡΠΎΠ²ΠΎΡΠΎΠΊΠ°
Π’Π΅ΡΠ°ΠΏΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠ°Ρ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Π²Π½ΡΡΡΠΈΠ°ΡΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΠΎΠ³ΠΎ Π²Π²Π΅Π΄Π΅Π½ΠΈΡ Π½Π΅ΠΉΡΠ°Π»ΡΠ½ΡΡ ΠΏΡΠΎΠ³Π΅Π½ΠΈΡΠΎΡΠ½ΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ, ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΡ ΠΈΠ· ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ ΠΏΠ»ΡΡΠΈΠΏΠΎΡΠ΅Π½ΡΠ½ΡΡ ΡΡΠ²ΠΎΠ»ΠΎΠ²ΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ, ΠΏΡΠΈ ΠΎΡΡΡΠΎΠΌ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠΌ ΠΈΡΠ΅ΠΌΠΈΡΠ΅ΡΠΊΠΎΠΌ ΠΈΠ½ΡΡΠ»ΡΡΠ΅ Ρ ΠΊΡΡΡ
Aim. Neural progenitor cells (NPC) are used for the development of cell therapies of neurological diseases. Their stereotaxic transplantation in the middle cerebral artery occlusion (MCAO) model imitating ischemic stroke results in symptom aleviation. However, exploration of less invasive transplantation options is essential, because stereotaxic transplantation is a complex procedure and can be applied to humans only by vital indications in a specialized neurological ward. The aim of the present study was to evaluate the efficacy of cell therapy of the experimental ischemic stroke by the intra-arterial transplantation of NPC.Materials and methods. NPC for transplantation (IPSC-NPC) were derived by two-stage differentiation of cells of a stable line of human induced pluripotent stem cells. Stroke modeling in rats was carried out by transitory 90 min endovascular MCAO by a silicon-tipped filament. NPC were transplanted 24 hours after MCAO. Repetitive magnetic resonance tomography of experimental animals was made with the Bruker BioSpin ClinScan tomograph with 7 Tl magnetic field induction. Animal survival rate and neurological deficit (using mNSS standard stroke severity scale) were evaluated at the 1st (before IPSC-NPC transplantation), 7th and 14th day after transplantation. Histological studies were carried out following standard protocols.Results. Intra-arterial transplantation of 7 Γ 105 IPSC-NPC in 1 ml at a constant 100 l/min rate in case of secured blood flow through the internal carotid artery did not cause brain capillary embolism, additional cytotoxic brain tissue edemas or other complications, while inducing increase of animal survival rate and enhanced revert of the neurological deficit. IPSC-NPC accumulation in brain after intra-arterial infusion was demonstrated. Some cells interacted with the capillary endothelium and probably penetrated through the blood-brain barrier.Conclusion. Therapeutic efficacy of the systemic, intra-arterial administration of NPC in ischemic stroke has been experimentally proven. A method of secure intra-arterial infusion of cell material into the internal carotid artery middle in rats has been developed and tested.Π¦Π΅Π»Ρ. ΠΠ΅ΠΉΡΠ°Π»ΡΠ½ΡΠ΅ ΠΏΡΠΎΠ³Π΅Π½ΠΈΡΠΎΡΠ½ΡΠ΅ ΠΊΠ»Π΅ΡΠΊΠΈ (ΠΠΠ) ΠΈΡΠΏΠΎΠ»ΡΠ·ΡΡΡΡΡ ΠΏΡΠΈ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠΊΠ΅ ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΠΉ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ Π½Π΅Π²ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ. ΠΡ
ΡΡΠ΅ΡΠ΅ΠΎΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π²Π²Π΅Π΄Π΅Π½ΠΈΠ΅ Π² ΠΌΠΎΠ·Π³ ΠΊΡΡΡ ΠΏΠΎΡΠ»Π΅ ΠΈΠΌΠΈΡΠΈΡΡΡΡΠ΅ΠΉ ΠΈΡΠ΅ΠΌΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΈΠ½ΡΡΠ»ΡΡ ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΈ ΠΎΠΊΠΊΠ»ΡΠ·ΠΈΠΈ ΡΡΠ΅Π΄Π½Π΅ΠΉ ΠΌΠΎΠ·Π³ΠΎΠ²ΠΎΠΉ Π°ΡΡΠ΅ΡΠΈΠΈ (ΠΠ‘ΠΠ) ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡ ΠΊ ΠΎΠ±Π»Π΅Π³ΡΠ΅Π½ΠΈΡ ΡΠΈΠΌΠΏΡΠΎΠΌΠ°ΡΠΈΠΊΠΈ. ΠΠ΄Π½Π°ΠΊΠΎ ΡΡΠ΅ΡΠ΅ΠΎΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π²Π²Π΅Π΄Π΅Π½ΠΈΠ΅ ΡΠ²Π»ΡΠ΅ΡΡΡ ΡΠ»ΠΎΠΆΠ½ΠΎΠΉ ΠΏΡΠΎΡΠ΅Π΄ΡΡΠΎΠΉ ΠΈ Π΄Π»Ρ Π»Π΅ΡΠ΅Π½ΠΈΡ Π±ΠΎΠ»Π΅Π·Π½Π΅ΠΉ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΎ ΡΠΎΠ»ΡΠΊΠΎ Π² ΡΠΏΠ΅ΡΠΈΠ°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΠΊΠ»ΠΈΠ½ΠΈΠΊΠ΅ ΠΏΠΎ ΠΆΠΈΠ·Π½Π΅Π½Π½ΡΠΌ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΈΡΠΌ, ΡΡΠΎ Π΄Π΅Π»Π°Π΅Ρ Π½Π΅ΠΎΠ±Ρ
ΠΎΠ΄ΠΈΠΌΡΠΌ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ ΠΌΠ΅Π½Π΅Π΅ ΡΡΠ°Π²ΠΌΠ°ΡΠΈΡΠ½ΡΡ
ΡΠΏΠΎΡΠΎΠ±ΠΎΠ² ΡΡΠ°Π½ΡΠΏΠ»Π°Π½ΡΠ°ΡΠΈΠΈ. Π¦Π΅Π»Ρ Π½Π°ΡΡΠΎΡΡΠ΅ΠΉ ΡΠ°Π±ΠΎΡΡ β ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΡ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΈΠ½ΡΡΠ»ΡΡΠ° ΠΏΡΡΠ΅ΠΌ Π²Π½ΡΡΡΠΈΠ°ΡΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΠΎΠ³ΠΎ Π²Π²Π΅Π΄Π΅Π½ΠΈΡ ΠΠΠ.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΠΠ Π΄Π»Ρ ΡΡΠ°Π½ΡΠΏΠ»Π°Π½ΡΠ°ΡΠΈΠΈ (ΠΠΠ‘Π-ΠΠΠ) ΠΏΠΎΠ»ΡΡΠ°Π»ΠΈ ΠΏΡΡΠ΅ΠΌ Π΄Π²ΡΡ
ΡΡΡΠΏΠ΅Π½ΡΠ°ΡΠΎΠΉ Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΡΠΎΠ²ΠΊΠΈ ΠΊΠ»Π΅ΡΠΎΠΊ ΡΡΠ°Π±ΠΈΠ»ΡΠ½ΠΎΠΉ Π»ΠΈΠ½ΠΈΠΈ ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΠΏΠ»ΡΡΠΈΠΏΠΎΡΠ΅Π½ΡΠ½ΡΡ
ΡΡΠ²ΠΎΠ»ΠΎΠ²ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°. ΠΠΎΠ΄Π΅Π»ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΈΠ½ΡΡΠ»ΡΡΠ° Ρ ΠΊΡΡΡ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΠΈΠ»ΠΎΡΡ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΡΠ°Π½Π·ΠΈΡΠΎΡΠ½ΠΎΠΉ (90 ΠΌΠΈΠ½) ΡΠ½Π΄ΠΎΠ²Π°ΡΠΊΡΠ»ΡΡΠ½ΠΎΠΉ ΠΠ‘ΠΠ ΡΠΈΠ»Π°ΠΌΠ΅Π½ΡΠΎΠΌ Ρ ΡΠΈΠ»ΠΈΠΊΠΎΠ½ΠΎΠ²ΡΠΌ Π½Π°ΠΊΠΎΠ½Π΅ΡΠ½ΠΈΠΊΠΎΠΌ. ΠΠ½ΡΡΡΠΈΠ°ΡΡΠ΅ΡΠΈΠ°Π»ΡΠ½Π°Ρ ΡΡΠ°Π½ΡΠΏΠ»Π°Π½ΡΠ°ΡΠΈΡ ΠΠΠ Π²ΡΠΏΠΎΠ»Π½ΡΠ»Π°ΡΡ ΡΠ΅ΡΠ΅Π· 24 ΡΠ°ΡΠ° ΠΏΠΎΡΠ»Π΅ ΠΠ‘ΠΠ. ΠΠ°Π³Π½ΠΈΡΠ½ΠΎ-ΡΠ΅Π·ΠΎΠ½Π°Π½ΡΠ½Π°Ρ ΡΠΎΠΌΠΎΠ³ΡΠ°ΡΠΈΡ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΡΡ
ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
Π² Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΠ΅ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»Π°ΡΡ Π½Π° ΠΠ -ΡΠΎΠΌΠΎΠ³ΡΠ°ΡΠ΅ ClinScan ΡΠΈΡΠΌΡ Bruker BioSpin Ρ ΠΈΠ½Π΄ΡΠΊΡΠΈΠ΅ΠΉ ΠΌΠ°Π³Π½ΠΈΡΠ½ΠΎΠ³ΠΎ ΠΏΠΎΠ»Ρ 7 Π’Π». ΠΠ° 1 (Π΄ΠΎ Π²Π²Π΅Π΄Π΅Π½ΠΈΡ ΠΠΠ‘Π-ΠΠΠ), 7 ΠΈ 14-Π΅ ΡΡΡΠΊΠΈ ΠΏΠΎΡΠ»Π΅ ΡΡΠ°Π½ΡΠΏΠ»Π°Π½ΡΠ°ΡΠΈΠΈ ΠΎΡΠ΅Π½ΠΈΠ²Π°Π»ΠΈΡΡ Π²ΡΠΆΠΈΠ²Π°Π΅ΠΌΠΎΡΡΡ ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
ΠΈ Π½Π΅Π²ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠΉ Π΄Π΅ΡΠΈΡΠΈΡ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΡΡΠ°Π½Π΄Π°ΡΡΠ½ΠΎΠΉ ΡΠΊΠ°Π»Ρ ΠΎΡΠ΅Π½ΠΊΠΈ ΡΡΠΆΠ΅ΡΡΠΈ ΠΈΠ½ΡΡΠ»ΡΡΠ° mNSS Π΄Π»Ρ Π³ΡΡΠ·ΡΠ½ΠΎΠ². ΠΠΈΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ, ΠΏΠΎΠ»ΡΠ·ΡΡΡΡ ΡΡΠ°Π½Π΄Π°ΡΡΠ½ΡΠΌΠΈ ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΠ½ΡΡΡΠΈΠ°ΡΡΠ΅ΡΠΈΠ°Π»ΡΠ½Π°Ρ ΡΡΠ°Π½ΡΠΏΠ»Π°Π½ΡΠ°ΡΠΈΡ ΠΠΠ‘Π-ΠΠΠ Π² Π΄ΠΎΠ·Π΅ 7 Γ 105 ΠΠΠ Π² 1 ΠΌΠ» Ρ ΡΠ°Π²Π½ΠΎΠΌΠ΅ΡΠ½ΠΎΠΉ ΡΠΊΠΎΡΠΎΡΡΡΡ100 ΠΌΠΊΠ»/ΠΌΠΈΠ½ ΠΈ ΠΏΠΎΠ΄Π΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ΠΌ ΠΊΡΠΎΠ²ΠΎΡΠΎΠΊΠ° ΠΏΠΎ Π²Π½ΡΡΡΠ΅Π½Π½Π΅ΠΉ ΡΠΎΠ½Π½ΠΎΠΉ Π°ΡΡΠ΅ΡΠΈΠΈ Π½Π΅ Π²ΡΠ·ΡΠ²Π°Π»Π° ΡΠΌΠ±ΠΎΠ»ΠΈΠΈ ΠΊΠ°ΠΏΠΈΠ»Π»ΡΡΠΎΠ² ΠΌΠΎΠ·Π³Π°, ΠΏΠΎΡΠ²Π»Π΅Π½ΠΈΡ Π½ΠΎΠ²ΡΡ
Π·ΠΎΠ½ ΡΠΈΡΠΎΡΠΎΠΊΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΎΡΠ΅ΠΊΠ° Π²Π΅ΡΠ΅ΡΡΠ²Π° Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠ³ΠΎ ΠΌΠΎΠ·Π³Π° ΠΈΠ»ΠΈ Π΄ΡΡΠ³ΠΈΡ
ΠΎΡΠ»ΠΎΠΆΠ½Π΅Π½ΠΈΠΉ ΠΈ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΠ»Π° ΠΊ Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΠΎΠΌΡ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΡ Π²ΡΠΆΠΈΠ²Π°Π΅ΠΌΠΎΡΡΠΈ ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
ΠΈ Π±ΠΎΠ»Π΅Π΅ Π±ΡΡΡΡΠΎΠΌΡ Π²ΠΎΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΈΡ Π½Π΅Π²ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΡΠ°ΡΡΡΠ°. ΠΡΠΎΠ΄Π΅ΠΌΠΎΠ½ΡΡΡΠΈΡΠΎΠ²Π°Π½ΠΎ Π½Π°ΠΊΠΎΠΏΠ»Π΅Π½ΠΈΠ΅ ΠΠΠ‘Π-ΠΠΠ Π² ΠΌΠΎΠ·Π³Π΅ ΠΏΠΎΡΠ»Π΅ ΠΈΡ
Π²Π½ΡΡΡΠΈΠ°ΡΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΠΈΠ½ΡΡΠ·ΠΈΠΈ. Π§Π°ΡΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΎΠ²Π°Π»Π° Ρ ΡΠ½Π΄ΠΎΡΠ΅Π»ΠΈΠ΅ΠΌ ΠΊΠ°ΠΏΠΈΠ»Π»ΡΡΠΎΠ² ΠΈ, Π²Π΅ΡΠΎΡΡΠ½ΠΎ, ΡΠΏΠΎΡΠΎΠ±Π½Π° ΠΏΡΠΎΠ½ΠΈΠΊΠ°ΡΡ ΡΠ΅ΡΠ΅Π· ΠΠΠ.ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΠΎΠ»ΡΡΠ΅Π½ΠΎ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠ΅ ΠΏΠΎΠ΄ΡΠ²Π΅ΡΠΆΠ΄Π΅Π½ΠΈΠ΅ ΡΠ΅ΡΠ°ΠΏΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΠΠ ΠΏΡΠΈ ΠΈΡΠ΅ΠΌΠΈΡΠ΅ΡΠΊΠΎΠΌ ΠΈΠ½ΡΡΠ»ΡΡΠ΅ ΠΏΡΠΈ ΡΠΈΡΡΠ΅ΠΌΠ½ΠΎΠΉ, Π²Π½ΡΡΡΠΈΠ°ΡΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΡΡΠ°Π½ΡΠΏΠ»Π°Π½ΡΠ°ΡΠΈΠΈ. ΠΡΡΠ°Π±ΠΎΡΠ°Π½ ΠΈ ΠΏΡΠΎΡΠ΅ΡΡΠΈΡΠΎΠ²Π°Π½ ΠΌΠ΅ΡΠΎΠ΄ Π±Π΅Π·ΠΎΠΏΠ°ΡΠ½ΠΎΠΉ Π²Π½ΡΡΡΠΈΠ°ΡΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΠΈΠ½ΡΡΠ·ΠΈΠΈ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π° Π² Π±Π°ΡΡΠ΅ΠΉΠ½ Π²Π½ΡΡΡΠ΅Π½Π½Π΅ΠΉ ΡΠΎΠ½Π½ΠΎΠΉ Π°ΡΡΠ΅ΡΠΈΠΈ Ρ ΠΊΡΡΡ
Evaluation of skin irritation in rats using simultaneous laser Doppler flowmetry and oxygenation monitoring
Β© 2016, Springer Science+Business Media New York.The possibility of assessment of skin irritation in rats with the use of simultaneous laser Doppler flowmetry (LDF) and oxygenation monitoring was investigated. The study was designed according to ISO 10993-10 using formaldehyde as a model irritant. 4-Hour application of 1Β % formaldehyde induced significant local increase in skin microcirculation for the first 2Β days by a factor of up to 2.4, whereas tissue oxygenation increased in much lower extent. The microcirculation was significantly inhibited by 10Β % formaldehyde following treatment and then it gradually increased for 24 and 48Β h of recovery period. The results correlated with visual evaluation of skin edema and erythema in treated zones. LDF/oxygenation signals provide early information on skin irritation and damage in rat model. The technique is valuable for preclinical/toxicological studies of chemical substances and medical materials
Evaluation of skin irritation in rats using simultaneous laser Doppler flowmetry and oxygenation monitoring
Β© 2016, Springer Science+Business Media New York.The possibility of assessment of skin irritation in rats with the use of simultaneous laser Doppler flowmetry (LDF) and oxygenation monitoring was investigated. The study was designed according to ISO 10993-10 using formaldehyde as a model irritant. 4-Hour application of 1Β % formaldehyde induced significant local increase in skin microcirculation for the first 2Β days by a factor of up to 2.4, whereas tissue oxygenation increased in much lower extent. The microcirculation was significantly inhibited by 10Β % formaldehyde following treatment and then it gradually increased for 24 and 48Β h of recovery period. The results correlated with visual evaluation of skin edema and erythema in treated zones. LDF/oxygenation signals provide early information on skin irritation and damage in rat model. The technique is valuable for preclinical/toxicological studies of chemical substances and medical materials
Evaluation of skin irritation in rats using simultaneous laser Doppler flowmetry and oxygenation monitoring
Β© 2016, Springer Science+Business Media New York.The possibility of assessment of skin irritation in rats with the use of simultaneous laser Doppler flowmetry (LDF) and oxygenation monitoring was investigated. The study was designed according to ISO 10993-10 using formaldehyde as a model irritant. 4-Hour application of 1Β % formaldehyde induced significant local increase in skin microcirculation for the first 2Β days by a factor of up to 2.4, whereas tissue oxygenation increased in much lower extent. The microcirculation was significantly inhibited by 10Β % formaldehyde following treatment and then it gradually increased for 24 and 48Β h of recovery period. The results correlated with visual evaluation of skin edema and erythema in treated zones. LDF/oxygenation signals provide early information on skin irritation and damage in rat model. The technique is valuable for preclinical/toxicological studies of chemical substances and medical materials