Effect of açai berry extract application on the bond strength to the bleached enamel using an experimental etch-and-rinse adhesive

To evaluate the bleached enamel shear bond strength (SBS) after antioxidants application using an experimental adhesive. Sixty sound human molars had their roots removed then cut into buccal and lingual halves and mounted in acrylic blocks. Enamel bleac

The effect of incorporating different concentrations of chlorhexidine digluconate on the degree of conversion of an experimental adhesive resin

The aim of this study was to evaluate the effect of chlorhexidine digluconate incorporation on the degree of conversion of an experimental adhesive resin. The experimental resin was prepared from 70 wt% bisphenol A glycerolate dimethacrylate, 30 wt% hydroxyethyl methacrylate, silanized SiO2 nanofillers, 0.5% of camphorquinone and ethyl 4-dimethylaminebenzoate (binary photo-initiator system). Five chlorhexidine digluconate concentrations (0, 0.5, 1, 2 and 4 wt%) were then incorporated into the experimental resin. Thirty Potassium Bromide pellets were prepared then divided into six groups (n=5/group), repre¬senting the tested adhesive resins (Single Bond 2, 0, 0.5, 1, 2 and 4 wt% chlohexidine-incorporated experimental adhesive resins), that were applied to the pellets without light-curing (uncured specimens). Another 30 pellets were prepared and treated with the previous materials then light-cured using LED light-curing device (cured specimens). Degree of conversion of the uncured and the cured specimens were evaluated using FTIR analysis. Adper Single Bond 2 showed the highest degree of conversion mean values followed by 0.5 wt% chlorhexidine concentration then 2 wt% followed by 4 wt% then 1 wt% concentrations, while 0 wt% concentration showed the lowest mean values. Chlorhexidine digluconate had slight significant influence on the efficiency of polymerization of the experimental adhesive resin

Genetic polymorphism of five genes associated with growth traits in goat

Genetic polymorphism studies in domestic animals aim at evaluating genetic variations within and across breeds mainly for conservation purposes. In this study, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect polymorphisms of five candidate genes in four Egyptian and Saudi goat breeds (Barki, Zaribi, Ardi and Masri), to detect the genotype of GH, IGF1, POUIF1, MSTN and BMP15 genes in the goat breeds and their allele frequencies. Results of GH gene which encloses a Haelll endonuclease restriction site show four unique PCR-RFLP banding patterns (genotypes AA, AB, CC and CD). The frequencies of the A allele in the samples from the goat breeds varied from 0.410 to 0.620. While  IGF-1gene revealed three fragments after digestion with Haelll with genotype AA, AB and BB and the  frequencies of allele A varied from 0.432 to 0.731. Furthermore, PCR-RFLP of POUIF1 gene showed two  fragments after digestion by Pst1 endonuclease with genotype TT and CC and the frequencies of allele T varied from 0.250 to 0.840. The MSTN gene revealed three fragments after digestion with DraI with genotype AA, BB and AB and the frequencies of allele A varied from 0.240 to 0.630. Meanwhile, the BMP15 gene revealed one fragments of 112 bp for AA after digestion with Hinf1 enzyme.Key words: Goats, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), GH, IGF-1, POUIF1, MSTN, BMP-15

Single nucleotide polymorphisms in the growth hormone receptor gene and Alu1 polymorphisms in the diacylglycerol acyltransferase 1 gene as related to meat production in sheep

Aim: This study aimed to investigate the polymorphisms in genes related to meat production, including growth hormone receptor (GHR) and diacylglycerol acyltransferase 1 (DGAT1) genes, in different breeds of sheep, including Barki, Najdi, and Harri. Materials and Methods: Blood samples were collected from 75 randomly selected healthy Barki, Najdi, and Harri breeds of sheep, with 25 samples per breed. GHR and DGAT1 genes were identified using a single nucleotide polymorphism assay followed by digestion with the restriction enzyme Alu1. Results: The analysis of the GHR gene sequence showed nucleotide substitutions at nt 69 in exon 10 (c.69 G > A); this mutation is considered a transition mutation. The sequences of detected SNPs in the GHR gene in the different sheep breeds were submitted to the GenBank database with accession numbers MG906773 to MG906781. The substitutions at exon 10 (c.69 G > A) results in an alteration to the amino acid (p. Lysine > Arginine). At c.69, the A allele frequency was 0.61, 0.59, and 0.54, while the G allele frequency was 0.39, 0.41, and 0.46, for Barki, Najdi, and Harri breeds, respectively. The genotype AG at nt 69 locus had the highest frequency in the Najdi and Harri sheep. The frequency of AG was 0.62, 0.61, and 0.64, while the frequency of AA was 0.30, 0.28, and 0.22, for Barki, Najdi, and Harri sheep, respectively. After digestion with the restriction enzyme AluI, the DGAT1 locus had two genotypes, CC and CT. The highest frequency, 0.88, was found for allele C, which was detected in Barki breed. The lowest frequency, 0.75, for the same allele was found for Harri. Conclusion: The detected CT genotype may explain the moderate intramuscular fat content and muscle marbling in the Barki sheep breed

Evaluation of different decontamination procedures on bond strength to sound and caries affected dentin using “no-wait” universal adhesive

Abstract Background Current study aimed to evaluate the effect of different decontamination procedures on micro-shear bond strength (μSBS) of sound (SoD) and caries-affected dentin (CAD) of two universal adhesives after blood-saliva contamination. Methods One hundred and eighty bovine anterior teeth were prepared and allocated into the respective groups according to tested dentin substrates [SoD, CAD], universal adhesives [Clearfil Bond Universal Quick (UBQ), All-Bond-Universal (ABU)], adhesive contamination stage [none, contamination before and after adhesives light-curing], and according to decontamination procedures [no decontamination, water rinsing, adhesive rebond, Ethylenediaminetetraacetic acid (ETDA) and chlorhexidine (CHX) application]. Universal adhesives were applied according to manufacturer instructions in self-etch (SE) bonding mode. Four composite microrods were built for each tooth. Specimens were kept in distilled water for 24 hours at 37°C before testing μSBS. Four-way ANOVA and Tukey HSD tests were used for data analysis. Results A statistically significant difference between contamination stages of both universal adhesives at different decontamination procedures for SoD and CAD. Highest μSBS was recorded for UBQ control group at SoD, while the least was recorded for light-cured ABU upon water rinsing decontamination procedure of CAD. Conclusions Proper cavity isolation is mandatory to avoid possible contamination which can dramatically affect μSBS. CHX is a potent cavity decontaminant that can restore different dentin substrates bond strength. EDTA presents a promising substitute. UBQ adhesive showed better bonding performance than ABU to both dentin substrates. Application of regular cavity decontamination approaches is highly advised in daily practice to avoid possible detrimental effect of accidental cavity contamination

Attitudes of Health Care Providers in Relation to Disability, Saudi Arabia

Background Among the main barriers that prevent people with disabilities from seeking help and support are negative attitudes of society in general and health care professionals in particular. However, only a few studies looked into the attitudes of health care providers toward people with disabilities in Saudi Arabia. This study aimed to investigate the attitudes of health care providers in relation to people with disabilities. Participants and Methods The attitudes toward disabled persons (ATDP) scale was administered among n = 1,033 health care providers in the Eastern Province of Saudi Arabia. The data were analyzed using JASP version 19 and Orange 3.26.0. Demographic data were analyzed using dummy encoding. Results The mean ATDP score for 1,033 respondents was 58.03 (p = 0.000). Multiple linear regression analysis showed that respondents aged 24 to 25 years have significantly less positive attitudes, compared to participants aged 18 to 20 years (p = 0.004). No sufficient evidence was found whether marital status significantly affects attitudes toward people with disabilities. In contrast, participants with three or more children have a significantly (p = 0.014) less positive attitude compared to those who do not have children. Conclusion This study found that, overall, health care professionals had slightly negative attitudes, according to the ATDP scale. However, younger respondents and those without children were more positive. Future research is recommended to collect more data on the significance of having children and its association with attitudes toward disabilities. More educational programs are recommended that can increase attitudes and awareness about people with disabilities

Two Red Sea Sponge Extracts (<i>Negombata magnifica</i> and <i>Callyspongia siphonella</i>) Induced Anticancer and Antimicrobial Activity

Bioactive compounds extracted from marine organisms showed several biological activities. The present study is an extension of our earlier studies where we assessed the antiproliferative and pro-apoptotic activities of ethanol, methylene chloride, ethyl acetate, acetone, and chloroform crude extracts of sponges: Negombata magnifica (NmE) and Callyspongia siphonella (CsE) against cancer cells. Herein, we are extending our previous findings on both sponge species depending on an alternative methanol extraction method with more advanced molecular biochemical insights as additional proof for anticancer and antimicrobial activity of N. magnifica and C. siphonella. Therefore, sponge specimens were collected during winter 2020 from the Dahab region at the Gulf of Aqaba. Each sponge was macerated with methanol to obtain the crude extracts; NmE and CsE. GC–MS analysis presented a total of 117 chemical compounds; 37 bioactive, 11 represented previously as constituents for a natural organism, and 69 had no biological activities. NmE dose-dependently inhibited the growth of HepG2, MCF-7, and Caco-2 carcinoma cell lines compared to CsE, which unfortunately has no antiproliferative activity against the same cancer cells. NmE was found to induce G0/G1 cell cycle arrest in HepG2 cells with its inhibition for CDK6, Cyclins D1, and E1 in HepG2, MCF-7, and Caco-2 cells. NmE also activated ROS production in HepG2 cells and induced apoptosis in HepG2, MCF-7, and Caco-2 cells via an increase in pro-apoptotic protein Bax, caspase-3, and cleavage PARP, and a decrease in anti-apoptotic protein BCL2. Unlike its anticancer potential, CsE exhibited clear superior results as an antimicrobial agent with a wider range against six microbial strains, whereas NmE showed a positive antibacterial activity against only two strains