Genotypic and phenotypic variation among Staphylococcus saprophyticus from human and animal isolates

<p>Abstract</p> <p>Background</p> <p>The main aim of this study was to examine the genotypic and phenotypic diversity of <it>Staphylococcus saprophyticus </it>isolates from human and animal origin.</p> <p>Findings</p> <p>In total, 236 clinical isolates and 15 animal isolates of <it>S. saprophyticus </it>were characterized in respect of the occurrence of 9 potential virulence genes and four surface properties. All strains were PCR positive for the regulatory genes <it>agr</it>, <it>sar</it>>it>A and <it>rot </it>as well as for the surface proteins UafA and Aas. Nearly 90% of the clinical isolates were found to possess the gene for the surface-associated lipase Ssp and 10% for the collagen binding MSCRAMM SdrI. All animal isolates were negative for<it>sdrI</it>. Lipolytic activity could be detected in 66% of the clinical and 46% of the animal isolates. Adherence to collagen type I was shown of 20% of the clinical strains and 6% of the strains of animal origin. Most <it>S. saprophyticus </it>strains showed hydrophobic properties and only few could agglutinate sheep erythrocytes.</p> <p>Conclusions</p> <p>We described a broad analysis of animal and human <it>S. saprophyticus </it>isolates regarding virulence genes and phenotypic properties such as lipase activity, hydrophobicity, and adherence. While <it>S. saprophyticus </it>strains from animal sources have prerequisites for colonization of the urinary tract like the D-serine-deaminase, out findings suggested that they need to acquire new genes e.g. MSCRAMMS for adherence like sdrI and to modulate their existing properties e.g. increasing the lipase activity or reducing hydrophobicity. These apparently important new genes or properties for virulence have to be further analyzed.</p

Role of mprF1 and mprF2 in the Pathogenicity of Enterococcus faecalis

Aujourd hui, Enterococcus faecalis est considéré comme l un des plus importants agents pathogènes causant des maladies nosocomiales. En raison de sa résistance innée et acquise aux antibiotiques, l identification de nouvelles cibles pour le traitement de cette bactérie est une grande priorité. Le facteur Multiple Peptide Résistance (MprF), qui a été décrit en premier chez Staphylococcus aureus, modifie le phosphatidylglycérol avec de la lysine et réduit ainsi la charge négative de l enveloppe cellulaire. Ceci a comme conséquence d augmenter la résistance aux peptides antimicrobiens cationiques (PAC). Deux gènes paralogues putatifs (mprF1 et mprF2) ont été identifiés chez E. faecalis par recherche BLAST en utilisant le gène décrit chez S. aureus. Une caractérisation de ces deux gènes d E. faecalis ainsi que des mécanismes conduisant à une résistance aux PAC, pourrait aider à développer des nouvelles stratégies thérapeutiques contre ce pathogène. Deux mutants de délétion et un double mutant ont été construits par recombinaison homologue chez E. faecalis. L analyse des phospholipides des membranes cytoplasmiques des deux mutants mprF1 et mprF2 par chromatographie sur couche mince a montré que seule l inactivation de mprF2 inhibe la synthèse de trois amino-phosphatidlyglycérol distincts (comme la Lysine-PG, l Alanine-PG et l Arginine-PG). De plus, le mutant mprF2 est également plus sensible aux PAC que la souche sauvage. La capacité de formation d un biofilm est généralement considérée comme un facteur important de virulence, ce qui est également le cas pour les entérocoques. Le mutant mprF2 montre une capacité accrue dans ce phénomène. Ceci semble être du à une augmentation de la concentration d ADN extracellulaire dans le biofilm formé par ce mutant. Curieusement, cette augmentation est indépendante d une autolyse. Le mutant mprF2 est également plus résistant à l opsonophagocytose. Cependant, le gène mprF2 ne joue aucun rôle dans les bactériémies de souris et les endocardites de rats.En revanche, aucun phénotype n a été trouvé pour un mutant mprF1 jusqu à présent. Cette mutation ne modifie ni la synthèse de l aminoacyl-PG en condition de laboratoire ni la résistance aux PAC et à l opsonophagocytose. Par conséquent, il semble que mprF2 soit le seul gène mprF fonctionnel chez E. faecalis. Néanmoins, contrairement à d autres bactéries, mprF2 ne semble pas être un facteur de virulence majeur pour cette espèce.Enterococcus faecalis is regarded nowadays as one of the most important nosocomial pathogens. Due to its innate and acquired resistance to antibiotics, identification of new targets for antimicrobial treatment of E. faecalis is a high priority. The multiple peptides resistance factor (MprF), which was first described in Staphylococcus aureus, modifies phosphatidylglycerol with lysine and reduces the negative charge of the membrane, thus increasing resistance to cationic antimicrobial peptides (CAMPs). Two putative mprF paralogs (mprF1 and mprF2) were identified in E. faecalis by Blast search using the well-described S. aureus gene as a lead. A better understanding of these two genes and mechanisms leads to enterococcal resistance to CAMPs might help designing therapeutic strategies against this bacteria. Two single deletion mutants and double mutant in E. faecalis were created by homologues recombination. Analysis of cell membrane phospholipids from both mutants by thin-layer chromatography showed that inactivation of mprF2 abolished the synthesis of three distinct amino-phosphatidylglycerol (mostly likely Lysin-PG, Alanine-PG and Argine-PG). The CAMPs testing assay demonstrated that the deletion mutant of mprF2 was more susceptible to CAMPs than the wild type. Biofilm formation is usually regarded as a virulence factor which provides an important way for enterococci to cause infections. Inactivation of mprF2 led to increase the biofilm formation which we showed that it was due to the accumulation of eDNA in the biofilm, but the release of eDNA is independent from autolysis. The mprF2 mutant was resistance to killing by opsonophagocytosis more than wild type. However, the mprF2 gene plays no role in bacteremia in mice and rat endocarditis. Our results showed that non polar effect mprF1 mutant does not affect in the synthesis of aminoacyl-PG in the laboratory condition. It also has no effect on susceptible to CAMPs, opsonic killing and autolysis. Therefore, it seems that mprF2 is the only functional mprF gene in E. faecalis in the laboratory condition. Unlike mprF found in other bacteria, mprF does not seem to be a major virulence factor in enterococci.CAEN-BU Sciences et STAPS (141182103) / SudocSudocFranceF

Financialization and productive models in the pharmaceutical industry

International audienc

MIOCENE TO PLIOCENE PALEOGEOGRAPHIC EVOLUTION OF TURKEY AND ITS SURROUNDINGS

Palaeogeographic studies suggest that the main mammal migration to Anatolia started in the late early to middle Miocene, following the closure of the Bitlis Ocean in southeastern Turkey. These animals, including hominoids, were able to mig-rate over the land bridge thus formed to Central and Western Anatolia where they settled. We discuss the Miocene to Pliocene palaeogeographic evolution of Turkey and its surroundings, in order to show what conditions were effective on this settlement

The effects of the North Anatolian Fault zone on the latest connection between Black Sea and Sea of Marmara

The development of the Strait of Istanbul is also one of the principal results of the tectonics which led to the evolution of the North Anatolian Fault Zone (NAFZ) in the Marmara Region 3.7 Ma ago. High resolution seismic profiles from the Marmara entrance of the Strait of Istanbul show a folding which occurred after the deposition of the parallel reflected Tyrrhenian sediments. Over the Tyrrhenian strata, a fondoform zone of a deltaic sequence and marine sediments of the latest sea level rising are present. These sediments also display syn-depositional folding. This situation implies that a local compressional stress field was created over the area probably since the Wurm Glacial age. This recent variation of the tectonic regime in the northern shelf of the Sea of Marmara may indicate a significant change in the development of the NAFZ through the Sea of Marmara. This variation of evolution of the NAFZ affected the latest development of the Strait of Istanbul via clockwise rotation of the Istanbul and Kocaeli peninsulas by right-lateral shearing between two zone bounding faults. This rotation has led to the development of NNE-SSW left-lateral faults in the Strait of Istanbul and local compressional and tensional areas explaining the compressional structures seen in the southern entrance of the Strait of Istanbul. Therefore, the latest Mediterranean-Black Sea connection was established by means of the sufficient deepening of the Bosphorus channel by a variation in the evolution of NAFZ through the Sea of Marmara. (C) 2002 Elsevier Science B.V. All rights reserved

Eastern Turkish high plateau as a small Turkic-type orogen: Implications for post-collisional crust-forming processes in Turkic-type orogens

Post-collisional magmatism may be generated by extensive crustal melting in Tibet-type collisional environments or by falling out of slabs from under giant subduction-accretion complexes in Turkic-type collisional orogens giving rise to decompression melting of the asthenospheric mantle replacing the removed oceanic lithosphere. In Turkic-type post-collisional magmatism, the magmatic products are dominantly alkalic to peralkalic and greatly resemble those of extensional regions giving rise to much confusion especially in interpreting old collisional orogenic belts. Such magmatic regions are also host to a variety of economically valuable ore deposits, including gold. One place in the world where today active, Turkic-type post-collisional magmatism is present is the eastern Anatolian high plateau, produced after the terminal Arabia/Eurasia collision in the late Miocene. The plateau is mostly underlain by the late Cretaceous to Oligocene East Anatolian Accretionary Complex, which formed south of the Rhodope-Pontide magmatic arc. This subduction-accretion complex has been further shortening since the collision, but it has also since been domed and became almost entirely covered by at least 15,000 km(3) of volcanic rocks. The volcanic rocks are calc-alkalic in the north, transitional in the middle, and alkalic in the south of the plateau. Where the crust is thinnest today (less than 38 km), the volcanics are derived almost entirely from an enriched mantle. The ages of the volcanics also become younger from north to south, from about 11 Ma to possibly 17th century AD. We interpret the origin of the magmatic rocks as the result of decompression melting of the asthenospheric mantle sucked towards the exposed base of the East Anatolian Accretionary Complex as the oceanic lithosphere beneath it fell out. The lower density of the hot asthospheric material was the cause of the doming. We believe that similar processes dominated the post-collisional tectonics of such vast Turkic-type orogens as the Altaids of Central Asia, the late Devonian-early Carboniferous Lachlan Belt in southeastern Australia, and the Pan-African collage of northeast Afro-Arabia. It is likely that Archaean collisions were dominated by Turkic-type post-collisional events rather than Tibetan ones that only became common in the Proterozoic. (C) 2008 Elsevier B.V. All rights reserved

Comparison of phenotypic methods for penicillinase detection in Staphylococcus aureus

ABSTRACTPenicillinase testing is required for Staphylococcus aureus isolates with a penicillin MIC of ≤0.12 mg/L. This study compared five phenotypic assays with a PCR for blaZ when testing 197 S. aureus isolates. The starch-iodine plate method and nitrocefin tests had low sensitivities of 42.9% and 35.7%, respectively. The cloverleaf assay and the penicillin zone-edge determination method had sensitivities of 67.8% and 71.4%, respectively, and these methods might be appropriate in many circumstances, but were not as sensitive as blaZ PCR

Paratethyan-Mediterranean connectivity in the Sea of Marmara region (NW Turkey) during the Messinian

The Sea of Marmara region is thought to have been a gateway between Paratethys and the Mediterranean since the Middle Miocene, and is therefore an important control on water mass exchange between the two realms. The Miocene successions in the northeastern Aegean and northwestern Marmara regions indicate that the first Mediterranean marine transgression to affect these areas occurred during the late Serravallian. In the northeastern Aegean region, frequent marine incursions occurred during the Tortonian and Messinian stages. The Messinian stage in this area is represented by a package of brackish- to fresh-water carbonates with some marine sandstone–siltstone interbeds (Alçıtepe Formation), which conformably overlies the Tortonian Kirazlı Formation. The Messinian sequence is overlain with an erosional contact by a shallow marine siliciclastic sequence (Göztepe Formation) of Zanclean age. With its brackish- to fresh-water carbonates and broadly constrained age, the Messinian sequence is interpreted as being coeval with the Upper Evaporite–Lago Mare sequence observed in western Mediterranean basins. In the western Marmara region, the Pontian (Messinian) Alçıtepe Formation consists of bioclastic and oolitic limestones with basal clastic rocks. It conformably overlies the fluvio-lacustrine siliciclastic sediments of the Middle to Upper Miocene Kirazlı Formation and is overlain by fluvio-lacustrine sediments of the Kimmerian (5.5–3.2 Ma) Truva and Tevfikiye formations with an erosional contact. The bioclastic limestones of the Alçıtepe Formation in the western Marmara region contain a molluscan and ostracod fauna that are endemic to Paratethys. These fauna indicate deposition in a shallow, brackish- to fresh-water environment. Faunal and paleomagnetic analyses of a section of the Alçıtepe Formation at Yenimahalle (Çanakkale) confirm that the formation is of Pontian age and represents chron C3r (6.04–5.24 Ma). The ostracod analysis indicates that during deposition of the Alçıtepe Formation, salinity increased from brackish in the lower part to more saline conditions in the upper part. Ostracod valves have low &lt;sup&gt;87&lt;/sup&gt;Sr / &lt;sup&gt;86&lt;/sup&gt;Sr values relative to coeval Late Miocene ocean water. This indicates that exchange between the Sea of Marmara region and the global ocean was restricted throughout this period. Fossil and Sr-isotope evidence suggests, however, that there was a Paratethyan–Marmara connection during the deposition of the lower part of the Alçıtepe Formation, with Paratethyan influence reaching the north Aegean. Connection via Marmara between Paratethys and the Mediterranean was not re-established until the late Aktchagylian (Late Pliocene). The re-connection was caused by both increased activity on the North Anatolian Fault and global sea level rise

Paratethyan-Mediterranean connectivity in the Sea of Marmara region (NW Turkey) during the Messinian

The Sea of Marmara region is thought to have been a gateway between Paratethys and the Mediterranean since the Middle Miocene, and is therefore an important control on water mass exchange between the two realms. The Miocene successions in the northeastern Aegean and northwestern Marmara regions indicate that the first Mediterranean marine transgression to affect these areas occurred during the late Serravallian. In the northeastern Aegean region, frequent marine incursions occurred during the Tortonian and Messinian stages. The Messinian stage in this area is represented by a package of brackish- to fresh-water carbonates with some marine sandstone–siltstone interbeds (Alçıtepe Formation), which conformably overlies the Tortonian Kirazlı Formation. The Messinian sequence is overlain with an erosional contact by a shallow marine siliciclastic sequence (Göztepe Formation) of Zanclean age. With its brackish- to fresh-water carbonates and broadly constrained age, the Messinian sequence is interpreted as being coeval with the Upper Evaporite–Lago Mare sequence observed in western Mediterranean basins. In the western Marmara region, the Pontian (Messinian) Alçıtepe Formation consists of bioclastic and oolitic limestones with basal clastic rocks. It conformably overlies the fluvio-lacustrine siliciclastic sediments of the Middle to Upper Miocene Kirazlı Formation and is overlain by fluvio-lacustrine sediments of the Kimmerian (5.5–3.2 Ma) Truva and Tevfikiye formations with an erosional contact. The bioclastic limestones of the Alçıtepe Formation in the western Marmara region contain a molluscan and ostracod fauna that are endemic to Paratethys. These fauna indicate deposition in a shallow, brackish- to fresh-water environment. Faunal and paleomagnetic analyses of a section of the Alçıtepe Formation at Yenimahalle (Çanakkale) confirm that the formation is of Pontian age and represents chron C3r (6.04–5.24 Ma). The ostracod analysis indicates that during deposition of the Alçıtepe Formation, salinity increased from brackish in the lower part to more saline conditions in the upper part. Ostracod valves have low &lt;sup&gt;87&lt;/sup&gt;Sr / &lt;sup&gt;86&lt;/sup&gt;Sr values relative to coeval Late Miocene ocean water. This indicates that exchange between the Sea of Marmara region and the global ocean was restricted throughout this period. Fossil and Sr-isotope evidence suggests, however, that there was a Paratethyan–Marmara connection during the deposition of the lower part of the Alçıtepe Formation, with Paratethyan influence reaching the north Aegean. Connection via Marmara between Paratethys and the Mediterranean was not re-established until the late Aktchagylian (Late Pliocene). The re-connection was caused by both increased activity on the North Anatolian Fault and global sea level rise