ウズラ卵白オボムコイドのラット脂質代謝に及ぼす影響

It is well known that trypsin inhibitors（TIs）alter exocrine pancreatic function. We found that dietary TIs decreased serum triglyceride levels in Wistar rats in a previous investigation. In this study，we tried to confirm the effect of TIs on lipid metabolism of Sprague-Dawley（SD）rats with Japanese quail ovomucoid（OM）as a TI. Five-week-old male SD rats were fed a 20% casein control diet or OM diets containing 0.1% OM，0.2% OM or 0.4% OM for 4 weeks. Rats had no change in food intake and growth rate between the control and OM fed groups. An OM fed group（0.4% OM）had lower serum triglyceride levels and no change in serum cholesterol levels. OM led to increased pancreatic protein content and trypsin and chymotrypsin activities in a dose dependent manner，but no difference in amylase activity. Liver fatty acid synthetase activity significantly decreased in OM fed groups. Therefore，we suggested that dietary OM caused increased pancreatic protein synthesis，which resulted in increased energy expenditure and decreased serum triglyceride level

マウス胸腺での脂肪細胞分化過程の解析

Logarithmic transcript-ratios of secreted frizzled-related protein 5（SFRP5）,lipocalin2（LC2） andPPAR γ（PR）, expressing in the thymus of mouse, were measured with aging by realtime PCR method.LogPR/SFRP5 and logPR/LC2 tended to increase for several weeks after birth and then became constan practically. Rate theory was applied to adipocyte-differential model, and rate constants were determined for theprocesses, where SFRP5- and LC2-transcribing cells differentiated into PR-transcribing cells. It was concludedthat SFRP5 was transcribed firstly, then LC2 in the process where stem cells differentiated into preadipocytes

Prognostic Significance of O 6-Methylguanine-DNA Methyltransferase Protein Expression in Patients with Recurrent Glioblastoma Treated with Temozolomide

Background: Temozolomide (TMZ) is active against newly diagnosed glioblastoma (GBM), and O 6-methylguanine-DNA methyltransferase (MGMT) is implicated in resistance to TMZ and nitrosoureas. We evaluated the efficacy and safety of the standard 5-day TMZ regimen in patients with recurrent GBM after initial therapy including nitrosourea-based chemotherapy, in conjunction with an analysis of the prognostic value of MGMT protein expression regarding response to TMZ and survival. Methods: From September 2003 to January 2007, 30 patients having recurrent GBM received 150–200 mg/m 2 /day of TMZ for five consecutive days every 28 days. Tumor tissue from 19 patients was analysed for MGMT protein expression using western blotting, and 17 of them were assessable for a response. Results: The overall response rate was 23.5 % (one complete response and three partial responses). Six patients had stable disease (35.3%). Median progression-free survival (PFS) time was 2.2 months, and median overall survival (OS) time was 9.9 months from the initiation of TMZ therapy. Patients with low MGMT protein expression had a significantly improved PFS (P 0.016) and OS (P 0.019) compared to those with high expression. Bot

Redox Potentials and HPLC Behavior of Cobalt and Iron Complexes with Pyridylazo Compounds

The redox potentials of cobalt and iron complexes with ten pyridylazo compounds, E0ML2 (ML2+/0; M: CoIII/II, FeIII/II; L-: pyridylazo compounds), have been determined in order to explore the difference in their reversed-phase HPLC behavior. The redox potentials of Co complexes were in the range of -0.62 - 0.03 V, while those of Fe complexes were -0.06 - 0.59 V relative to 0.20 V for ferricinium/ferrocene. The redox potentials of both the Co and Fe complexes were linearly correlated to the basicities of the ligands. The correlation was quantitatively explained by a difference in dependence of the stabilities of MIII and MII complexes on the ligand basicities. The complex of [CoIIIL2]+ or [FeIIL2] with any compound injected in the reversed-phase HPLC system was detected without any change in the composition. When [CoIIL2] was injected, only those complexes having the highest potentials of E0CoL2 ≅ 0.0 V were detected as [CoIIL2], while other complexes having lower potentials gave a peak of [CoIIIL2]+. When [FeIIIL2]+ was injected, only complexes having the lowest potentials of E0FeL2 ≅ 0.0 V were detected as [FeIIIL2]+, while others having higher potentials gave a peak of [FeIIL2]

Time course and spacial distribution of UV effects on human skin in organ culture

Apoptosis plays an important role in eliminating cells from populations when cells have been exposed to UV irradiation and damaged. Studies of cells in culture have provided some details of the mechanisms involved when stress response genes act after exposure to UV irradiation and other environmental stresses. However, little is known about the responses of intact sections of human skin growing in organ culture to UV irradiation. In the work reported here, it was found that the response of organ-cultured human skin after exposure to UV irradiation is different than the response of cultured cells. At wavelengths below 300 nm, the action spectrum obtained from organ-cultured skin samples showed a lower sensitivity than that observed at 300 nm, indicating that the overlying stratum corneum and upper epidermal cell layers had probably caused a selective absorption of incident UV radiation at some wavelengths. At 3 hours after UV irradiation, p53 was phosphorylated at Ser 15 and Ser 46, and accumulated in the cell nuclei, notably after exposure to 280-320 nm wavelengths. Accumulations of Bax, active Caspase-3 and cleaved PARP were detected in apoptotic cells at 24 hours post-exposure, along with a reduction of Bcl-2 levels, notably after exposure to 300-365 nm light. This difference in apoptotic responses may result from the characteristics of the different irradiation wavelengths used, and from details in the skin\u27s structure. The data obtained in this study using an organ-culture system utilized direct measurements of the biological effects of different wavelengths of UV lights