Production of FAME biodiesel in E. coli by direct methylation with an insect enzyme.

Most biodiesel currently in use consists of fatty acid methyl esters (FAMEs) produced by transesterification of plant oils with methanol. To reduce competition with food supplies, it would be desirable to directly produce biodiesel in microorganisms. To date, the most effective pathway for the production of biodiesel in bacteria yields fatty acid ethyl esters (FAEEs) at up to ~1.5 g/L. A much simpler route to biodiesel produces FAMEs by direct S-adenosyl-L-methionine (SAM) dependent methylation of free fatty acids, but FAME production by this route has been limited to only ~16 mg/L. Here we employ an alternative, broad spectrum methyltransferase, Drosophila melanogaster Juvenile Hormone Acid O-Methyltransferase (DmJHAMT). By introducing DmJHAMT in E. coli engineered to produce medium chain fatty acids and overproduce SAM, we obtain medium chain FAMEs at titers of 0.56 g/L, a 35-fold increase over titers previously achieved. Although considerable improvements will be needed for viable bacterial production of FAMEs and FAEEs for biofuels, it may be easier to optimize and transport the FAME production pathway to other microorganisms because it involves fewer enzymes

IRAS studies of galactic supershells

Using IRAS Skyflux images and a new catalog of OB stars in the Cygnus region, a complete infrared supershell surrounding the Cyg OB1 Association was identified. This supershell is seen as a conspicuous, well-defined 2 deg x 5 deg region deficient of IR emission, with a limb-brightened edge and dimensions of about 50 x 130 pc at 1.5 kpc. The shell's elongated morphology is consistent with OB-star subclustering over the approximately 10(exp 6) yr age of the bubble. With a parent star cluster still visible (10 O stars between 25 and 45 solar mass, 3 - 4 Wolf-Rayet stars, and the possibility of 3 - 5 more massive stars that died as supernovae) the Cyg OB1 supershell is an excellent object for studying the formation and evolution of Galactic supershells. A discrepancy between the less than or equal to 1 Myr bubble age estimated from its size and the 5 Myr cluster turnoff age (45 solar mass) may require non-coeval massive star formation to explain the number of post-main-sequence stars and limit the number of past supernovae