9 research outputs found
Two novel acyl sucroses from <i style="">Petunia nyctaginiflora</
648-650Two acyl sucroses, isolated
from Petunia nyctaginiflora Jussieu
(Solanaceae) have been characterized chemically as 2,3,4-tri(5-methylhexanoyl)--D-glucopyranosyl--D-6-acetyl fructofuranoside
4 and 2,3,4-tri(6-methylheptanoyl)--D-glucopyranosyl--D-6-acetyl
fructofuranoside 6, on the basis of
chemical and spectroscopic evidence
Cytotoxic constituents of <i>Abutilon indicum</i> leaves against U87MG human glioblastoma cells
<div><p>The study was aimed to identify cytotoxic leads from <i>Abutilon indicum</i> leaves for treating glioblastoma. The petroleum ether extract, methanol extract (AIM), chloroform and ethyl acetate sub-fractions (AIM-C and AIM-E, respectively) prepared from AIM were tested for cytotoxicity on U87MG human glioblastoma cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. These extracts exhibited considerable activity (IC<sub>50</sub> values of 42.6–64.5 μg/mL). The most active AIM-C fraction was repeatedly chromatographed to yield four known compounds, methyl trans-<i>p</i>-coumarate (<b>1</b>), methyl caffeate (<b>2</b>), syringic acid (<b>3</b>) and pinellic acid (<b>4</b>). Cell viability assay of <b>1</b>–<b>4</b> against U87MG cells indicated <b>2</b> as most active (IC<sub>50</sub> value of 8.2 μg/mL), whereas the other three compounds were much less active. Interestingly, compounds <b>1</b>–<b>4</b> were non-toxic towards normal human cells (HEK-293). The content of <b>2</b> in AIM-C was estimated as 3% by HPLC. Hence, presence of some more active substances besides methyl caffeate (<b>2</b>) in AIM-C is anticipated.</p></div
Antiparkinsonian Effects of Aqueous Methanolic Extract of Hyoscyamus niger Seeds Result From its Monoamine Oxidase Inhibitory and Hydroxyl Radical Scavenging Potency
Hyoscyamus species is one of the four plants
used in Ayurveda for the treatment of Parkinson’s disease
(PD). Since Hyoscyamus niger was found to contain negligible
levels of L-DOPA, we evaluated neuroprotective
potential, if any, of characterized petroleum ether and
aqueous methanol extracts of its seeds in 1-methyl-4-phenyl-
1,2,3,6-tetrahydropyridine (MPTP) model of PD in
mice. Air dried authenticated H. niger seeds were
sequentially extracted using petroleum ether and aqueous
methanol and were characterized employing HPLC-electrochemistry
and LCMS. Parkinsonian mice were treated
daily twice with the extracts (125–500 mg/kg, p.o.) for two
days and motor functions and striatal dopamine levels were
assayed. Administration of the aqueous methanol extract
(containing 0.03% w/w of L-DOPA), but not petroleum ether extract, significantly attenuated motor disabilities
(akinesia, catalepsy and reduced swim score) and striatal
dopamine loss in MPTP treated mice. Since the extract
caused significant inhibition of monoamine oxidase activity
and attenuated 1-methyl-4-phenyl pyridinium (MPP?)-
induced hydroxyl radical (�OH) generation in isolated
mitochondria, it is possible that the methanolic extract of
Hyoscyamus niger seeds protects against parkinsonism in
mice by means of its ability to inhibit increased �OH
generated in the mitochondria