36 research outputs found
Room-Temperature Fluorescence Lifetime of Pseudoisocyanine (PIC) J Excitons with Various Aggregate Morphologies in Relation to Microcavity Polariton Formation
The results of room-temperature fluorescence lifetime measurements are reported for the excitation of J aggregates (Js) of pseudoisocyanine chloride (PIC-Cl) prepared in potassium polyvinyl sulfate (PVS) polymer thin films, their aqueous solutions, and NaCl aqueous solutions. Variations of the microscopic morphologies of the aggregates were investigated. The results show that fluorescence decay features correlated to the morphology change. The observed fluorescence lifetime and quantum efficiency of PIC J aggregates (PIC-Js) in a NaCl aqueous solution were 310 ps and 28%, respectively. The lifetime of the fibril-shaped macroaggregates prepared in PVS thin films was below the instrumental time resolution of 5 ps, and the efficiency decreased to below 3%. The results indicate that PIC-Js prepared with PVS polymers have an increased nonradiative contribution to the excitation deactivation process. In particular, macro-Js with isolated fibril-shaped structures revealed nonradiative pathway(s) that are closely associated to the specific packaging morphology of the constituent meso-Js. The possibility of a destructive effect on the formation of cavity-polaritons is also discussed
Eccentric Figure-Eight Coils for Transcranial Magnetic Stimulation
Previously we proposed an eccentric figure-eight coil that can cause threshold stimulation in the brain at lower driving currents. In this study, we performed numerical simulations and magnetic stimulations to healthy subjects for evaluating the advantages of the eccentric coil. The simulations were performed using a simplified spherical brain model and a realistic human brain model. We found that the eccentric coil required a driving current intensity of approximately 18% less than that required by the concentric coil to cause comparable eddy current densities within the brain. The eddy current localization of the eccentric coil was slightly higher than that of the concentric coil. A prototype eccentric coil was designed and fabricated. Instead of winding a wire around a bobbin, we cut eccentric-spiral slits on the insulator cases, and a wire was woven through the slits. The coils were used to deliver magnetic stimulation to healthy subjects; among our results, we found that the current slew rate corresponding to motor threshold values for the concentric and eccentric coils were 86 and 78âA/”s, respectively. The results indicate that the eccentric coil consistently requires a lower driving current to reach the motor threshold than the concentric coil. Future development of compact magnetic stimulators will enable the treatment of some intractable neurological diseases at home. Bioelectromagnetics. 35:55â65, 2015. © 2014 Wiley Periodicals, Inc.ArticleBIOELECTROMAGNETICS. 36(1):55-65 (2015)journal articl
Preferred site occupation of 3d atoms in NixFe4âxN(x=1 and 3) films revealed by x-ray absorption spectroscopy and magnetic circular dichroism
X-ray absorption spectroscopy (XAS) and x-ray magnetic circular dichroism measurements were performed at the Ni and Fe L2,3 absorption edges for NixFe4âxN(x=1 and 3) epitaxial films. Spectral line-shape analysis and element-specific magnetic moment evaluations are presented. Shoulders at approximately 2 eV above the Ni L2,3 main peaks in the XAS spectrum of Ni3FeN were interpreted to originate from hybridization of orbitals between Ni 3d at face-centered (II) sites and N 2p at body-centered sites, while such features were missing in NiFe3N film. Similar shoulders were observed at Fe L2,3 edges in both films. These results indicate that the orbitals of Ni atoms did not hybridize with those of N atoms in the NiFe3N film. Hence, Ni atoms preferentially occupied corner (I) sites, where the hybridization was weak because of the relatively long distance between Ni at I sites and N atoms. The relatively large magnetic moment deduced from sum-rule analysis of NiFe3N also showed a good agreement with the presence of Ni atoms at I sites
X-ray magnetic circular dichroism of ferromagnetic Co4N epitaxial films on SrTiO3(001) substrates grown by molecular beam epitaxy
5-nm thick Co4N layers capped with 3-nm thick Au layers were grown epitaxially on SrTiO3(001) substrates by molecular beam epitaxy using solid Co and a radio-frequency NH3 plasma. Spin and orbital magnetic moments of the Co4N layers were estimated using x-ray magnetic circular dichroism (XMCD) measurements at 300âK. The site-averaged Co 3d spin magnetic moment is evaluated to be about 1.4âÎŒB, which is smaller than that predicted theoretically (1.58âÎŒB). The element-specific XMCD intensities for the Co L3 edge and N K edge show that the magnetic moment is induced at the N atoms
Electronic structures and magnetic moments of Co3FeN thin films grown by molecular beam epitaxy
We evaluated electronic structures and magnetic moments in Co3FeN epitaxial films on SrTiO3(001). The experimentally obtained hard x-ray photoemission spectra of the Co3FeN film have a good agreement with those calculated. Site averaged spin magnetic moments deduced by x-ray magnetic circular dichroism were 1.52âÎŒ B per Co atom and 2.08âÎŒ B per Fe atom at 100âK. They are close to those of Co4N and Fe4N, respectively, implying that the Co and Fe atoms randomly occupy the corner and face-centered sites in the Co3FeN unit cell
Room-Temperature Fluorescence Lifetime of Pseudoisocyanine (PIC) J Excitons with Various Aggregate Morphologies in Relation to Microcavity Polariton Formation
The results of room-temperature fluorescence lifetime measurements are reported for the excitation of J aggregates (Js) of pseudoisocyanine chloride (PIC-Cl) prepared in potassium polyvinyl sulfate (PVS) polymer thin films, their aqueous solutions, and NaCl aqueous solutions. Variations of the microscopic morphologies of the aggregates were investigated. The results show that fluorescence decay features correlated to the morphology change. The observed fluorescence lifetime and quantum efficiency of PIC J aggregates (PIC-Js) in a NaCl aqueous solution were 310 ps and 28%, respectively. The lifetime of the fibril-shaped macroaggregates prepared in PVS thin films was below the instrumental time resolution of 5 ps, and the efficiency decreased to below 3%. The results indicate that PIC-Js prepared with PVS polymers have an increased nonradiative contribution to the excitation deactivation process. In particular, macro-Js with isolated fibril-shaped structures revealed nonradiative pathway(s) that are closely associated to the specific packaging morphology of the constituent meso-Js. The possibility of a destructive effect on the formation of cavity-polaritons is also discussed
The delta opioid receptor agonist KNTâ127 relieves innate anxietyâlike behavior in mice by suppressing transmission from the prelimbic cortex to basolateral amygdala
Abstract Aim Excitatory projections from the prelimbic cortex (PL) to the basolateral nucleus of the amygdala (BLA) are implicated in the regulation of anxietyâlike behaviors, and we previously demonstrated that anxiolyticâlike effects of the selective deltaâopioid receptor (DOP) agonist KNTâ127 is involved in suppressing glutamate neurotransmission in the PL. Here, we investigated the mechanisms underlying the anxiolyticâlike effect of KNTâ127 in mice by combining optogenetic stimulation of the PLâBLA pathway with behavioral analyses. Methods Fourâweekâold male C57BL/6J mice received bilateral administration of adenoâassociated virus (AAV)2âCaMKIIaâhChR2(H134R)âenhanced yellow fluorescent protein (EYFP) into the PL to induce expression of the lightâactivated excitatory ionic channel ChR2. Subsequently, an optic fiber cannula connected to a wireless photoâstimulator was implanted into the BLA for optogenetic PLâBLA pathway stimulation. We evaluated innate anxiety using the elevated plus maze (EPM) and open field (OF) tests as well as learned anxiety using the contextual fear conditioning (CFC) test. Results Optogenetic activation of the PLâBLA pathway enhanced anxietyâlike behaviors in the EPM and OF, while prior subcutaneous administration of KNTâ127 (10âmg/kg) reduced this anxiogenic effect. In contrast, optogenetic activation of the PLâBLA pathway had no significant effect on conditioned fear. Conclusion Our findings indicate that the PLâBLA circuit contributes to innate anxiety and that the anxiolyticâlike effects of KNTâ127 are mediated at least in part by suppression of PLâBLA transmission. The PL deltaâopioid receptor may thus be an effective therapeutic target for anxiety disorders
Duloxetine Inhibits Microglial P2X4 Receptor Function and Alleviates Neuropathic Pain after Peripheral Nerve Injury.
P2X4 receptors (P2X4R) are a family of ATP-gated non-selective cation channels. We previously demonstrated that activation of P2X4R in spinal microglia is crucial for neuropathic pain, a highly debilitating chronic pain condition, suggesting that P2X4R is a potential therapeutic target for treating neuropathic pain. Thus, the identification of a compound that has a potent inhibitory effect on P2X4R is an important clinical challenge. In the present study, we screened a chemical library of clinically approved drugs and show for the first time that duloxetine, a serotonin and noradrenaline reuptake inhibitor, has an inhibitory effect on rodent and human P2X4R. In primary cultured microglial cells, duloxetine also inhibited P2X4R-, but not P2X7R-, mediated responses. Moreover, intrathecal administration of duloxetine in a model of neuropathic pain produced a reversal of nerve injury-induced mechanical allodynia, a cardinal symptom of neuropathic pain. In rats that were pretreated with a serotonin-depleting agent and a noradrenaline neurotoxin, the antiallodynic effect of duloxetine was reduced, but still remained. Based on these results, we suggest that, in addition to duloxetine's primary inhibitory action on serotonin and noradrenaline transporters, an inhibitory effect on P2X4R may be involved at least in part in an antiallodynic effect of intrathecal duloxetine in a model of neuropathic pain