Regulation of antioxidant enzyme activities in female rat brain by ovarian steroids

The sex steroids produce their effects by acting on numerous target tissues and organs, such as the reproductive organs, bone tissue and cartilage, peripheral blood vessels as well as the central nervous system (CNS). In our studies we have monitored the change of enzyme activity of the antioxidant (AO) system in the brain of female rats depending on the ovarian steroids. We have chosen it as a new parameter that might represent an important indicator of the changes within the CNS, bearing in mind the biological importance of the enzymes of the AO system. The experimental results of our study indicate that the enzyme activity of the AO system in the brain tissue of female rats shows a certain dependence on the concentration of ovarian hormones, progesterone and estradiol, in the organism. Study of the activity of the enzymes of the AO system in the brain of female rats depending on the influence of ovarian hormones can answer whether the action of ovarian steroids on the CNS includes maintenance of a dynamic equilibrium of free radicals in the neurons

Activity of antioxidative defense enzymes in the blood of patients with liver echinococcosis

Background/Aim. Chronic echinococcocal disease is the parasite human disease caused by the penetration of larval (asexual) stages of the canine tapeworm (Echinococcus granulosus) in the liver of humans. After the penetration of the parasite, the host organism react by activating complement- depending immune response. The aim of this study was to elucidate the influence of larval form of Echinococcus granulosus in the liver on the activity of antioxidative defense enzymes in the blood of patients before and after the surgical intervention. Methods. We investigated the activity of antioxidative defense enzymes: copper/zinc containing superoxide dismutase (CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and glutathione-S-transferase (GST) in the blood of patients before and after the surgical intervention in respect to the controls, clinically healthy persons. Results. Our results showed that the activity of the GSH-Px was significantly decreased in the plasma of the patients with echinocococal disease before the surgery in respect to the controls. The activity of GST was significantly higher in the blood of the patients after the surgery in comparison to the controls. Conclusion. Chronic liver echinoccocal disease caused significant changes of some antioxidative defense enzymes, first of all Se-dependent enzyme GSH-Px, which could be a suitabile biomarker in the biochemical evaluation of the disease. This work represents a first comprehensive study of the activity of antioxidative defense enzymes in cronic liver echinococcocosis in the patients before and after the surgical intervention in respect to the clinically healthy persons

Time course of hematological parameters in bleeding-induced anemia

In order to investigate daily changes of hematological parameters in bleeding-induced anemia, we treated Wistar albino male rats by daily bleeding (1.5-2 mL of blood from the tail vein for eight days). Blood samples were taken before (on day zero) and on the first to eighth days of bleeding. The values of hematocrit, hemoglobin, and erythrocyte count decreased significantly after the second, sixth, and second days of bleeding, respectively. The number of leuko­cytes and platelets, as well as Heinz body levels, increased significantly after the third and second days of treatment. The percentage of reticulocytes increased significantly from the second day and attained the maximum level (32.55 ± 0.96%) on the eighth day

Effects of cisplatin on lipid peroxidation and the glutathione redox status in the liver of male rats: The protective role of selenium

The role of oxidative stress in cisplatin (CP) toxicity and its prevention by pretreatment with selenium (Se) was investigated. Male Wistar albino rats were injected with a single dose of cisplatin (7.5 mg CP/kg b.m., i.p.) and selenium (6 mg Se/kg b.m, as Na2SeO3, i.p.) alone or in combination. The results suggest that CP intoxication induces oxidative stress and alters the glutathione redox status: reduced glutathione (GSH), oxidized glutathione (GSSG) and the GSH/GSSG ratio (GSH RI), resulting in increased lipid peroxidation (LPO) in rat liver. The pretreatment with selenium prior to CP treatment showed a protective effect against the toxic influence of CP on peroxidation of the membrane lipids and an altering of the glutathione redox status in the liver of rats. From our results we conclude that selenium functions as a potent antioxidant and suggest that it can control CP-induced hepatotoxicity in rats

Effects of cisplatin on lipid peroxidation and the glutathione redox status in the liver of male rats: The protective role of selenium

The role of oxidative stress in cisplatin (CP) toxicity and its prevention by pretreatment with selenium (Se) was investigated. Male Wistar albino rats were injected with a single dose of cisplatin (7.5 mg CP/kg b.m., i.p.) and selenium (6 mg Se/kg b.m, as Na2SeO3, i.p.) alone or in combination. The results suggest that CP intoxication induces oxidative stress and alters the glutathione redox status: reduced glutathione (GSH), oxidized glutathione (GSSG) and the GSH/GSSG ratio (GSH RI), resulting in increased lipid peroxidation (LPO) in rat liver. The pretreatment with selenium prior to CP treatment showed a protective effect against the toxic influence of CP on peroxidation of the membrane lipids and an altering of the glutathione redox status in the liver of rats. From our results we conclude that selenium functions as a potent antioxidant and suggest that it can control CP-induced hepatotoxicity in rats.U našoj studiji ispitivana je uloga oksidacionog stresa u toksičnosti cisplatina (SR) i njegova prevencija pretretmanom selenom (Se). Mužjaci Wistar albino pacova su inicirani jednom dozom cisplatina (7.5 mg CP/kg t.m., i.p.) i selena (6 mg Se/kg t.m, kao Na2SeO3, i.p.) pojedinačno ili u kombinaciji. Rezultati pokazuju da intoksikacija SR uzrokuje oksidacioni stres i promenu glutation redoks statusa: redukovanog (GSH), oksidovanog (GSSG) i GSH/GSSG indeksa (GSH RI), kao i povećanje lipidne peroksidacije (LPO) u jetri pacova. Tretman Se koji je prethodio tretmanu SR pokazao je zaštitne efekte protiv toksičnog delovanja SR na peroksidaciju lipida membrane i promenu glutation redoks statusa u jetri pacova. Na osnovu naših rezultata zaključujemo da Se, deluje kao snažan antioksidans i da može imati ulogu u kontroli SR indukovane hepatotoksičnosti kod pacova.Projekat ministarstva br. 143035

Aktivnost superoksid-dismutaze tokom maturacije kulture ćelija b-16 melanoma miša

The C3 clone of B-16 mouse melanoma was cultured for 1, 6 and 9 days and analyzed. The changes which are not directly linked to melanogenesis in the B-16 / C3 cultures during their maturation were characterized. Early (1 day) confluent (6 days) and old (9 days) cell cultures are distinguished by their leucine aminopeptidase (LAP) and α-naphthyl acetate esterase (ANAE) isoenzyme patterns. Both quantitative and qualitative changes in LAP and ANAE isoenzyme can be observed during culture maturation. There is an increase in the activity of the enzyme copper, zinc-containing superoxide-dismutase (CuZn SOD). The increaase in the CuZn SOD enzyme activity might be related to B-16/C3 cell melanogenesis and / or to differentiation.C3 klonovi B-16 melanoma miša su gajeni u kulturi 1, 6 i 9 dana i potom analizirani. Izvršena je karakterizacija promena B-16/C3 ćelijskih kultura tokom njihove maturacije koje nisu direktno povezane sa melanogenezom. Pokazano je da se kulture u početnoj fazi (1 dan), konfluentnoj fazi (6 dana) i kasnoj fazi (9 dana) ćelijskog rasta razlikuju na osnovu izoenzimskih spektara leucin-aminopeptidaze i alfa-naftil-acetat-esteraze. Tokom razvitka ćelijskih kultura B-16/C3 zapažene su kvantitativne i kvalitativne promene leucin-aminopeptidaze i alfa-naftil-acetat-esteraze u njihovim izoenzimskim spektrima. Utvrđeno je da postoji porast aktivnosti bakar-cink superoksid-dismutaze tokom starenja kulture. Porast aktivnosti bakar-cink superoksid-dismutaze može biti povezan sa melanogenezom i/ili diferencijacijom B-16/C3 ćelija

Activity of antioxidative defense enzymes in the blood of patients with liver echinococcosis

Background/Aim. Chronic echinococcocal disease is the parasite human disease caused by the penetration of larval (asexual) stages of the canine tapeworm (Echinococcus granulosus) in the liver of humans. After the penetration of the parasite, the host organism react by activating complement- depending immune response. The aim of this study was to elucidate the influence of larval form of Echinococcus granulosus in the liver on the activity of antioxidative defense enzymes in the blood of patients before and after the surgical intervention. Methods. We investigated the activity of antioxidative defense enzymes: copper/zinc containing superoxide dismutase (CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and glutathione-S-transferase (GST) in the blood of patients before and after the surgical intervention in respect to the controls, clinically healthy persons. Results. Our results showed that the activity of the GSH-Px was significantly decreased in the plasma of the patients with echinocococal disease before the surgery in respect to the controls. The activity of GST was significantly higher in the blood of the patients after the surgery in comparison to the controls. Conclusion. Chronic liver echinoccocal disease caused significant changes of some antioxidative defense enzymes, first of all Se-dependent enzyme GSH-Px, which could be a suitabile biomarker in the biochemical evaluation of the disease. This work represents a first comprehensive study of the activity of antioxidative defense enzymes in cronic liver echinococcocosis in the patients before and after the surgical intervention in respect to the clinically healthy persons.Uvod/Cilj. Ehinokokusna bolest je parazitno oboljenje koje nastaje prodorom larvene forme pseće pantljičare (Echinococcus granulosus) u jetru čoveka, pri čemu dolazi do reakcije domaćina koji se brani od infektivnog agensa aktivacijom sistema komplementa i komplement-zavisnim imunim odgovorom. Cilj ovog rada bio je da se utvrdi da li razvoj larvenog oblika pseće pantljičare (Echinococcus granulosus) u jetri ima uticaja na aktivnost enzima sistema zaštite od oksidacionih oštećenja u krvi obolelih pre i posle operativnog zahvata. Metode. Ispitivane su promene aktivnosti enzima sistema zaštite od oksidacionih oštećenja: bakar/cink sadržavajuće superoksid-dismutaze (CuZn SOD), katalaze (CAT), glutation-peroksidaze (GSH-Px), glutation-reduktaze (GR) i glutation-S-transferaze (GST) u krvi obolelih od ehinokokusne bolesti jetre pre i posle operacije u odnosu na kontrolne, klinički zdrave osobe. Rezultati. Ustanovljen je statistički značajan pad aktivnosti enzima GSH-Px u plazmi bolesnika obolelih od ehinokokusne bolesti jetre pre operacije u odnosu na kontrolu. Aktivnost enzima GST bila je statistički značajno povećana u krvi obolelih od hronične ehinokokusne bolesti jetre posle operacije u odnosu na kontrolne vrednosti. Zaključak. Hronična ehinokokusna bolest jetre izaziva promene aktivnosti nekih enzima sistema zaštite od oksidacionih oštećenja, pre svega Se-zavisnog enzima GSH-Px, što može predstavljati dobar biomarker u daljoj biohemijskoj evaluaciji ove bolesti. Prema dostupnim podacima ovaj rad predstavlja prvu studiju aktivnosti enzima sistema zaštite od oksidacionih oštećenja kod obolelih od hronične ehinokokusne bolesti jetre pre i posle operacije u odnosu na kontrolne, klinički zdrave osobe.nul

Activity of antioxidative defense enzymes in the blood of patients with liver echinococcosis

Background/Aim. Chronic echinococcocal disease is the parasite human disease caused by the penetration of larval (asexual) stages of the canine tapeworm (Echinococcus granulosus) in the liver of humans. After the penetration of the parasite, the host organism react by activating complement- depending immune response. The aim of this study was to elucidate the influence of larval form of Echinococcus granulosus in the liver on the activity of antioxidative defense enzymes in the blood of patients before and after the surgical intervention. Methods. We investigated the activity of antioxidative defense enzymes: copper/zinc containing superoxide dismutase (CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and glutathione-S-transferase (GST) in the blood of patients before and after the surgical intervention in respect to the controls, clinically healthy persons. Results. Our results showed that the activity of the GSH-Px was significantly decreased in the plasma of the patients with echinocococal disease before the surgery in respect to the controls. The activity of GST was significantly higher in the blood of the patients after the surgery in comparison to the controls. Conclusion. Chronic liver echinoccocal disease caused significant changes of some antioxidative defense enzymes, first of all Se-dependent enzyme GSH-Px, which could be a suitabile biomarker in the biochemical evaluation of the disease. This work represents a first comprehensive study of the activity of antioxidative defense enzymes in cronic liver echinococcocosis in the patients before and after the surgical intervention in respect to the clinically healthy persons.Uvod/Cilj. Ehinokokusna bolest je parazitno oboljenje koje nastaje prodorom larvene forme pseće pantljičare (Echinococcus granulosus) u jetru čoveka, pri čemu dolazi do reakcije domaćina koji se brani od infektivnog agensa aktivacijom sistema komplementa i komplement-zavisnim imunim odgovorom. Cilj ovog rada bio je da se utvrdi da li razvoj larvenog oblika pseće pantljičare (Echinococcus granulosus) u jetri ima uticaja na aktivnost enzima sistema zaštite od oksidacionih oštećenja u krvi obolelih pre i posle operativnog zahvata. Metode. Ispitivane su promene aktivnosti enzima sistema zaštite od oksidacionih oštećenja: bakar/cink sadržavajuće superoksid-dismutaze (CuZn SOD), katalaze (CAT), glutation-peroksidaze (GSH-Px), glutation-reduktaze (GR) i glutation-S-transferaze (GST) u krvi obolelih od ehinokokusne bolesti jetre pre i posle operacije u odnosu na kontrolne, klinički zdrave osobe. Rezultati. Ustanovljen je statistički značajan pad aktivnosti enzima GSH-Px u plazmi bolesnika obolelih od ehinokokusne bolesti jetre pre operacije u odnosu na kontrolu. Aktivnost enzima GST bila je statistički značajno povećana u krvi obolelih od hronične ehinokokusne bolesti jetre posle operacije u odnosu na kontrolne vrednosti. Zaključak. Hronična ehinokokusna bolest jetre izaziva promene aktivnosti nekih enzima sistema zaštite od oksidacionih oštećenja, pre svega Se-zavisnog enzima GSH-Px, što može predstavljati dobar biomarker u daljoj biohemijskoj evaluaciji ove bolesti. Prema dostupnim podacima ovaj rad predstavlja prvu studiju aktivnosti enzima sistema zaštite od oksidacionih oštećenja kod obolelih od hronične ehinokokusne bolesti jetre pre i posle operacije u odnosu na kontrolne, klinički zdrave osobe.nul

Biochemical and ultrastructural changes in the liver of European perch (Perca fluviatilis L.) in response to cyanobacterial bloom in the Gruža reservoir

We investigated the biochemical and ultrastructural changes in the liver of the freshwater fish, European perch (Perca fluviatilis), in response to Aphanizomenon flos-aquae bloom in the Gruža Reservoir, Serbia. The activities of total manganese- and copper zinc-containing superoxide dismutase (Tot SOD, Mn-SOD, Cu/Zn-SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and biotransformation phase II enzyme glutathione-S-transferase (GST), as well as concentrations of total glutathione (GSH) and sulfhydryl (-SH) groups were examined before and during the bloom period. Mn-SOD activity was significantly higher, while the activities of Cu/Zn-SOD, CAT and GSH-Px and the concentration of the -SH groups were significantly lower during the bloom. The ultrastructure of the liver revealed necrotic and apoptotic damage to the hepatocytes during the bloom period. Our work represents the first study to report the influences of an Aphanizomenon flos-aquae bloom in the Gruža Reservoir on antioxidant biomarkers and on histopathological alterations in the liver of the freshwater fish European perch (Perca fluviatilis)

Supplementary data for the article: Prokić, M. D.; Borković-Mitić, S. S.; Krizmanić, I. I.; Mutić, J. J.; Trifković, J. Đ.; Gavrić, J. P.; Despotović, S. G.; Gavrilović, B. R.; Radovanović, T. B.; Pavlović, S. Z.; et al. Bioaccumulation and Effects of Metals on Oxidative Stress and Neurotoxicity Parameters in the Frogs from the Pelophylax Esculentus Complex. Ecotoxicology 2016, 25 (8), 1531–1542. https://doi.org/10.1007/s10646-016-1707-x

Supplementary material for: [https://doi.org/10.1007/s10646-016-1707-x]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2340