DIAGNOSIS OF PERSISTENT BVD IN ONE DAY OLD AND OLDER THAN 30 DAYS CALVES WITH RAPID KIT TESTS

Bovine Viral Diarrhoea (BVD) is an important disease of cattle which causes alimentary, respiratory and reproductive disorders and serious economic losses. Today, eradication of disease is compulsory for the increase of dairies and achieving more healthier herds. In cattle, if the calves are infected with BVD from the uterus in the first trimester of pregnancy, they are born persistent infected (PI) antigen(+). The PI calves are smaller or normal in apperance than normal calves. Persistent infected (PI) calves spread the virus throughout their lives and infect their surroundings. BVD causes early embrionic death, calves born with anomalies and immunosuppression in infected animals. In this study 200 calves between 1–30 days old from Burdur province are screened with BVD rapid test kit. In the screening, 13 calves (6.5%) are found BVDV ag(+). The gender of PI calves are found 4% female (8/200) and 2.5% male (5/200). In the 5% of PI calves (10/200), respiratory and alimentary problems were clinically seen. The antigen(+) animals are put out of the herd and vaccination protocols are recommended. In this study, diagnosing the disease can be made rapidly by both clinicians and farmers, also putting the virus spreading persistent calves out of herd without losing time and a successful struggle can be made against BVD, are shown

FREQUENCY OF DEA 1.1 ANTIGEN IN GERMAN SHEPHERDS

Objectives: A correlation between blood type and the incidence of certain diseases in humans was established many years ago. It may be assumed that similar correlations could occur in dogs. Therefore, establishing the geographical and breed distribution of erythrocyte surface antigens in dogs may be of academic interest and useful in practice too. The aim of the study was the assessment of DEA 1.1 frequency among German Shepherds in the Lublin region of Poland.Material and methods: Our study involved 20 German Shepherds from Eastern Poland. The presence of blood antigen was detected using a quick immunochromatographic test. In 9 cases a donor–recipient compatibility test was also performed.Results: The antigen DEA 1.1 was detected in 15% of dogs. A clear, macroscopically visible agglutination did not occur in any of the donor–recipient compatibility tests.Conclusion: The breed studied may therefore be recognised as a suitable blood donor.

Selected serum biochemical parameters and acute phase protein levels in a herd of Saanen goats showing signs of pregnancy toxaemia

ABSTRACT: The purpose of this study was to examine selected serum biochemical parameters and acute phase protein levels in a herd of Saanen goats showing signs of pregnancy toxaemia. Seventy five female goats were used and divided into three groups. Group 1 (n = 57) (blood serum glucose levels were within the physiological range), Group 2 (n = 11) (serum glucose values were low) and Group 3 (n = 7) (serum glucose values were high). Goats in Groups 2 and 3 were diagnosed with pregnancy toxaemia. Apart from serum glucose, β-hydroxybutyrate (BHB), triglycerides, blood pH, calcium (Ca), sodium (Na), potassium (K), aspartate aminotransferase (AST), alanine aminotransferase (ALT), haptoglobin (Hp), serum amyloid A (SAA) and tumour necrosis factor-α (TNF-α) were measured in all animals. In Group 3 average Hp and SAA values were found to be significantly (P < 0.001) higher than in Groups 1 and 2, and also higher in Group 2 than in Group 1. Acute phase proteins in goats with pregnancy toxaemia may be used in the course and the prognosis of the disease. The evaluation of acute phase proteins is useful and also quicker in cases of suspected pregnancy intoxication

diabetes mellitus

Objectives: In this study, we investigated clinically, pathologically, and immunohistochemically the effect of insufficient short-acting insulin treatment on streptozotocin (STZ)-induced diabetes mellitus in rats.Methods: Three groups composed of 10 rats each were studied as follows: (1) a group that received only STZ (50 mg/kg) (STZ group); (2) a group that received 50 mg/kg STZ and, after 12 hours, 8 IU of short-acting insulin treatment (STZ + INS group), repeated every night for 5 days; and (3) a control group. Ketonuria and blood glucose levels were examined every day. Blood was obtained from 2 rats from each group, and necropsy was performed every day during the 5-day period.Results: Hyperglycemia was observed in the STZ and STZ + INS groups 24 hours after, but levels were higher in the STS + INS group than those in the STZ-only group. Histopathology was similar in the STZ and STZ + INS groups, and degeneration was observed in both groups, but immunohistochemistry revealed a more severe reduction in insulin-secreting cells in the STZ + INS group than that in the STZ group. There were no hyperglycemia and histopathological or immunochemical alteration in the control group.Conclusions: This study showed that insufficient short-acting insulin treatment can increase the diabetogenic effect of STZ in rats

Ameliorative effect of certolizumab on experimentally induced acute necrotic pancreatitis in rats

OBJECTIVE: The effects of Certolizumab, a pegylated monoclonal antibody to tumor necrosis factor α, on experimentally induced acute pancreatitis (AP) were examined. METHODS: Thirty-six Wistar Albino male rats were randomly divided into four groups. Group I was the control group and no medication administered to this group. Group II was the Certolizumab group, and 100 ml/kg serum physiologic administered into the biliopancreatic duct and a single dose of 10 µg Certolizumab was simultaneously administered intraperitoneally. Acute pancreatitis was induced with a retrograde injection of 3% Na taurocholate into the common biliopancreatic duct in the study (Group III) and treatment (Groups IV) groups. Rats were sacrificed 72 hours later. Serum amylase, lipase, lactate dehydrogenase activities, along with pancreatic histo-pathology, were examined. RESULTS: Certolizumab treatment significantly decreased serum amylase, lipase, and LDH levels; histopathologically edema, hemorrhage, parenchymal necrosis, fat necrosis, and infiltration scores; immunohistochemically MDA, MPO, TNF-α and Caspase-3 activity. CONCLUSION: The results support the idea that certolizumab might be beneficial for the severity of AP. © 2019 Associacao Medica Brasileira. All rights reserved

necrotic pancreatitis in rats

OBJECTIVE: The effects of Certolizumab, a pegylated monoclonal antibody to tumor necrosis factor alpha, on experimentally induced acute pancreatitis (AP) were examined