7 research outputs found
EPR Relaxation-Enhancement-Based Distance Measurements on Orthogonally Spin-Labeled T4-Lysozyme
ISSN:1439-4227ISSN:1439-763
Distance determination from dysprosium induced relaxation enhancement: a case study on membrane-inserted WALP23 polypeptides
RIDME Spectroscopy with Gd(III) Centers
The
relaxation induced dipolar modulation enhancement (RIDME) technique
is applied at W-band microwave frequencies around 94 GHz to a pair
of GdÂ(III) complexes that are connected by a rodlike spacer, and the
extraction of the interspin distance distribution is discussed. A
dipolar pattern derived from RIDME experimental data is a superposition
of Pake-like dipolar patterns corresponding to the fundamental dipolar
interaction and higher harmonics thereof. Intriguingly, the relative
weights of the stretched patterns do not depend significantly on mixing
time. As much larger modulation depths can be achieved than in double
electron–electron resonance distance measurements at the same
frequency, GdÂ(III)–GdÂ(III) RIDME may become attractive for
structural characterization of biomacromolecules and biomolecular
complexes