Antifungal acetylinic thiophenes from Tagetes minuta: potential biopesticide

Apart from thiophenes, which possess wide range of biocidal activity, aerial parts of Tagetes sp. contain essential oil. Oil components were reported to have antifungal activity, thus making whole plant of Tagetes very useful for exploiting as natural fungistatic agent. In the present study, Tagetes minuta grown in north western Himalayan condition were evaluated for its potential for use as antifungal agent. Flower essential oil showed minimal antifungal activity. Whereas, leaf essential oil was found signifi cant antifungal activity against three phytopathogenic fungi out of eight tested fungi. ED50 values were 165, 175 and 110 μg mL-1 against Rhizoctonia solani, Sclerotinia sclerotiorum and Sclerotium rolfsii, respectively. Thiophene rich extract of Tagetes minuta was found comparatively lesser active (ED50: 233-484 μg mL-1) than leaf essential oil against the same fungi. The present study shows that essential oil from leaves and thiophene rich extracts from marigold roots have signifi cantly good antifungal activity against a number of soil borne and foliar plant pathogens. The easy availability of these plants makes it an attractive potential candidate for development of natural fungicide

Insect growth regulatory activity of Thevetia nerifolia Juss. against Spodoptera litura (Fab.)

Screening for insect growth regulatory activity (IGR) of Thevetia nerifolia leaf extracts were evaluated against Spodoptera litura (Fab.). Methanol extract of leaves provided 53.8 % larval mortality, 29.6 % pupation and 22.3 % adult emergence at 2.5 % concentration level. The extract was further subfractioned with solvents of different polarity in search of better IGR activity and chloroform extract was found to be most active in terms of larval mortality (27.5-61.5 %), pupation (28.4-60.2 %) and adult emergence (19.8-52.8 %). GI50 of the extract was recorded to be 3.02 %. Activity was attributed to the glycosides present in the extract

Valorisation of black carrot pomace: microwave assisted extraction of bioactive phytoceuticals and antioxidant activity using Box–Behnken design

The present study compares three methods viz. microwave assisted extraction (MAE), ultrasonic-assisted extraction (UAE) and conventional solvent extraction (CSE) for extraction of phenolic compounds from black carrot pomace (BCP). BCP is the major by-product generated during processing and poses big disposal problem. Box–Behnken design using response surface methodology was employed to investigate and optimize the MAE of phenolics, antioxidant activity and colour density from BCP. The conditions for maximum recovery of polyphenolics were: microwave power (348.07 W), extraction time (9.8 min), solvent–solid ratio (19.3 mL/g) and ethanol concentration (19.8%). Under these conditions, the extract contained total phenolic content of 264.9 ± 10.02 mg gallic acid equivalents (GAE)/100 mL, antioxidant capacity (AOC) of 13.14 ± 1.05 lmol Trolox equivalents (TE)/mL and colour density of 68.63 ± 5.40 units. The total anthocyanin content at optimized condition was 753.40 ± 31.6 mg/L with low % polymeric colour of 7.40 ± 0.42. At optimized conditions, MAE yielded higher colour density (68.63 ± 5.40), polyphenolic content (264.9 ± 10.025 mg GAE/100 mL) and AOC (13.14 ± 1.05 lmol TE/mL) in a short time as compared to UAE and CSE. Overall results clearly indicate that MAE is the best suited method for extraction in comparison to UAE and CSE. The phenolic rich extract can be used as an effective functional ingredient in foods

Optimization of the use of cellulolytic enzyme preparation for the extraction of health promoting anthocyanins from black carrot using response surface methodology

Anthocyanin-rich extracts from black carrots are being considered as a candidate replacer for the red colour in processed foods. The present investigation optimizes the extraction of anthocyanins with high phenolic content and low degradation parameters from black carrots using a cellulolytic multi-enzyme preparation known as Viscozyme. The optimized conditions for Viscozyme using a Box-Behnken design (BBD) of response surface methodology (RSM) were as follow: temperature (50.2 ◦C), extraction time (58.4 min) and enzyme concentration (0.20%). The predicted value of anthocyanins content was 1380 mg/L, which was near to the optimized experimental value of 1375 mg/L. The extracted anthocyanins based on above mentioned conditions exhibited the lowest degradation parameters such as degradation index (DI) of (0.86), browning index of (BI) (1.31) and were characterized with cyanidin 3-sinapoylxylosylglucosylgalactoside as being the most abundant. The findings clearly reveal that Viscozyme-assisted extraction (VAE) is the best approach for extracting superior quality extracts from black carrots with high anthocyanin and other phenolic component concentrations

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Not AvailableFinger millet (Eleusine coracana) is an important staple food grain in North Western Himalayan Region (NWHR) of India. In traditional process, it takes five hours effort for threshing and pearling of 100 kg of finger millet grain. The objective of the present study was to find out the optimum machine parameters for efficient threshing and pearling operations of finger millets using artificial neural network (ANN) and genetic algorithm (GA) techniques. The machine performances like threshing capacity (32.4 kg/h against 33.21 kg/h predicted), threshing efficiency (98.41 % against 97.90 % predicted), pearling capacity (65.0 kg/h against 65.12 kg/h predicted) and pearling efficiency (98.3 % against 98.60 % predicted) were evaluated for its optimal design parameters viz., number canvas strip on drum periphery (8), overhanging width of canvas strip (5.17 mm) and peripheral drum speed (7.97 m/s) for finger millet.ICA

Sorption isotherms of barnyard millet grain and kernel

Not AvailableThe moisture sorption isotherms of grain and kernel of barnyard millet (Echinochloa frumentacea) were determined at 20, 30, 40, and 50 °C. A gravimetric static method was used under 0.112–0.964 water activity (a w) range for the determination of sorption isotherms. The models were compared using the coefficient of determination (r 2), reduced chi-square (χ 2) values, and on the basis of residual plots. In grain, modified Chung–Pfost (r 2 > 0.99; χ 2  0.99; χ 2  0.99 and χ 2 < 0.55) for describing the adsorption and desorption of the kernel. The isosteric heat, calculated using Clausius–Clapeyron equation, was varied between 46.76 and 61.71 kJ g−1 mol−1 at moisture levels 7–21% (d.b.) for grain and 47.11–63.52 kJ g−1 mol−1 at moisture level between 4% and 20% (d.b.) for kernel. The monolayer moisture content values ranged from 4.3% to 6% d.b. in the case of adsorption of barnyard millet grain and 5.2–6.6% d.b. in the case of desorption at the temperature ranges of 50–20 °C. The monolayer moisture values of barnyard millet kernel ranged from 4.4% to 6.67% d.b. in adsorption and 4.6% to 7.3% d.b. in desorption in the temperature ranges of 50–20 °CICA

Moisture-dependent properties of barnyard millet grain and kernel

Not AvailableThe geometric mean diameter, sphericity, grain surface area, 1000 grain mass, true density (toluene displacement method), terminal velocity, dynamic angle of repose, coefficient of internal friction, coefficient of static friction at different surfaces (sun mica, canvas and mild steel surfaces), specific deformation and rupture energy of the grain were found to increase 12.21%, 4.79%, 30.47%, 30.75%, 6.74%, 32.99%, 127.05%, 60%, 18.57%, 34–67%, 69.2% and 88.87% respectively at increase of moisture content from 0.065 to 0.265 kg kg−1 dry matter. However, true density (proximate composition method), bulk density, interstices and rupture force of grain was found to be decrease 8.64%, 20.1%, 86.49% and 21.17% respectively at increase of moisture content. Similar trend was observed for barnyard kernel also. True density (toluene displacement method) was found lower as compared to true density (proximate composition method) at all experimental moisture range indicated that the presence of void space inside the grain and kernel.ICA

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Not AvailableThe goal of the present study was to investigate the bioactive molecules (anthocyanins and fatty acids) present in the aril of pomegranate. Major anthocyanins present in the aril of pomegranate were identified by HRMS as delphinidin 3,5-diglucoside, cyanidin 3,5-diglucoside, pelargonidin 3,5-diglucoside, cyanidin 3-glucoside and delphinidin 3-glucoside. In-vitro study revealed that bioaccessibility of anthocyanin in duodenal condition was varied between 7.3 and 9.7%. Encapsulation enhances the bioaccessibility of both the phenolics to some extent in gastric as well as duodenal condition. Seed oil contains significant amount of unsaturated fatty acids especially x-5 fatty acids. Geometrical isomers of x-5 fatty acids were also identified by GC–MS. The spray dried anthocyanin formulation has potential for food application.Not Availabl