Mucormycosis: Issues, Pathophysiology, Diagnosis and Treatment

Mucormycosis (Black fungus) is an infection caused by opportunistic fungi of order Mucorales affecting the immune-compromised individuals. Cases of Aspergillus and Candida (White fungus) were also reported. The cases of fungal co-infection in COVID-19 patients are increasing globally affecting mainly diabetic patients and individuals that have undergone corticosteroid therapy. Iron metabolism and hypoxia plays an important role in pathophysiology of this disease. Mucormycosis causes thrombosis of blood vessels hindering the blood circulation causing black discoloration of affected site. Histopathological examination of specimen is the most effective way of its diagnosis. Surgical removal of affected site is the best treatment practice in rhino-orbital and cutaneous cases whereas lipid formulation of Amphotericin B is effective in other forms of fungal infection

Socio-economic analysis of coffee growers in Gulmi district of Nepal

Coffee is a major plantation cash crop of hills of Nepal. Gulmi is one of the popular districts for coffee production and export in Nepal. This research is an attempt to assess the production potentiality and profitability of coffee in Gulmi district. This study was carried out in four rural municipalities of Gulmi district namely Ruru rural municipality, Dhurkot rural municipality, Satyawoti rural municipality and Musikot municipality. A total of 100 samples (25 from each rural municipality) were selected using simple random sampling technique. Face to face (FtF) interview method was used to collect primary data using pretested semi-structured questionnaire. The economic indicators of coffee production like gross revenue, gross margin, benefit-cost ratio (BCR) and profitability index (PI) were calculated. BCR and PI were found to be 2.84± 0.59 and 2.50± 1.25, respectively. Gross margin per ropani was calculated to be NRs. 15675.29 ± 7189.72. The contribution of coffee in total household income was 12% in Gulmi showing it to be one of the major influencing commodities. The major production problem was found to be insect attack in the district while the major marketing problem was the low market price. The insights of this research were that coffee has the potentiality to uplift the rural income of Gulmi but at the same time there were poor extension services for coffee growers such that farmers had shown dissatisfaction towards coffee enterprise. Therefore, effective package of production and value chain monitoring should be introduced by the Nepal government in order to address the production and marketing constraints of coffee producers

A Case Report on Canine Transmissible Venereal Tumor

A male Japanese spitz (3 years) was brought at Himalayan Animal Rescue Trust (HART), Pokhara with a complaint of swollen gums and loss of appetite. A lobulated tumorous mass was seen at the gingival region on physical examination. Diagnosis and treatment of condition detected in the dog was the major objective. Impression smear of tumor cell was prepared and was observed under oil immersion microscope (100x). Microscopic examination shows the presence of vacuolations within the cytoplasm and the condition was diagnosed to be CTVT. Chemotherapy was performed using the most effective cytostatic agents I.e. Vincristine sulphate (once a week, I/v). The chemotherapy was repeated for 3 doses till the tumor gets completely regressed. The condition was resolved after third session of chemotherapy. Myelosuppression and gastrointestinal effects like vomiting are the major complications of using vincristine

Small Molecule Inhibitors of 15-PGDH Exploit a Physiologic Induced-Fit Closing System

15-prostaglandin dehydrogenase (15-PGDH) is a negative regulator of tissue stem cells that acts via enzymatic activity of oxidizing and degrading PGE2, and related eicosanoids, that support stem cells during tissue repair. Indeed, inhibiting 15-PGDH markedly accelerates tissue repair in multiple organs. Here we have used cryo-electron microscopy to solve the solution structure of native 15-PGDH and of 15-PGDH individually complexed with two distinct chemical inhibitors. These structures identify key 15-PGDH residues that mediate binding to both classes of inhibitors. Moreover, we identify a dynamic 15-PGDH lid domain that closes around the inhibitors, and that is likely fundamental to the physiologic 15-PGDH enzymatic mechanism. We furthermore identify two key residues, F185 and Y217, that act as hinges to regulate lid closing, and which both inhibitors exploit to capture the lid in the closed conformation, thus explaining their sub-nanomolar binding affinities. These findings provide the basis for further development of 15-PGDH targeted drugs as therapeutics for regenerative medicine

The COVID‐19 Pandemic Not Only Poses Challenges, but Also Opens Opportunities for Sustainable Transformation

The COVID-19 pandemic has impacted social, economic, and environmental systems worldwide, slowing down and reversing the progress made in achieving the Sustainable Development Goals (SDGs). SDGs belong to the 2030 Agenda to transform our world by tackling humankind's challenges to ensure well-being, economic prosperity, and environmental protection. We explore the potential impacts of the pandemic on SDGs for Nepal. We followed a knowledge co-creation process with experts from various professional backgrounds, involving five steps: online survey, online workshop, assessment of expert's opinions, review and validation, and revision and synthesis. The pandemic has negatively impacted most SDGs in the short term. Particularly, the targets of SDG 1, 4, 5, 8, 9, 10, 11, and 13 have and will continue to have weakly to moderately restricting impacts. However, a few targets of SDG 2, 3, 6, and 11 could also have weakly promoting impacts. The negative impacts have resulted from impeding factors linked to the pandemic. Many of the negative impacts may subside in the medium and long terms. The key five impeding factors are lockdowns, underemployment and unemployment, closure of institutions and facilities, diluted focus and funds for non-COVID-19-related issues, and anticipated reduction in support from development partners. The pandemic has also opened a window of opportunity for sustainable transformation, which is short-lived and narrow. These opportunities are lessons learned for planning and action, socio-economic recovery plan, use of information and communication technologies and the digital economy, reverse migration and “brain gain,” and local governments' exercising authorities

Building a responsive website

The purpose of the thesis was to research and study the concept of creating a responsive web design and implement the study to build a website design that is optimised for adaptive web design. The theoretical part of the thesis work explains the concepts of creating responsive web pages and elements to create an adaptive design, including flexible text, flexible grid, and adaptive media, as well as a theory on media queries. For practical purposes, a website as a prototype was built, tested on various screen resolution devices, and evaluated in terms of adaptive design. The development of the project involved various steps. Initially, the requirements of the project were analysed. Based on the requirements, the project was designed and developed using Virtual Studio Code, a software development tool. As an outcome, a website that can adapt content according to the screen size was created. HTML5, CSS3, SASS, VS Code, NPM, CSS grid, and CSS flexbox were the technologies used in the project

Applications of Microfluidics and Organ-on-a-Chip in Cancer Research

Taking the life of nearly 10 million people annually, cancer has become one of the major causes of mortality worldwide and a hot topic for researchers to find innovative approaches to demystify the disease and drug development. Having its root lying in microelectronics, microfluidics seems to hold great potential to explore our limited knowledge in the field of oncology. It offers numerous advantages such as a low sample volume, minimal cost, parallelization, and portability and has been advanced in the field of molecular biology and chemical synthesis. The platform has been proved to be valuable in cancer research, especially for diagnostics and prognosis purposes and has been successfully employed in recent years. Organ-on-a-chip, a biomimetic microfluidic platform, simulating the complexity of a human organ, has emerged as a breakthrough in cancer research as it provides a dynamic platform to simulate tumor growth and progression in a chip. This paper aims at giving an overview of microfluidics and organ-on-a-chip technology incorporating their historical development, physics of fluid flow and application in oncology. The current applications of microfluidics and organ-on-a-chip in the field of cancer research have been copiously discussed integrating the major application areas such as the isolation of CTCs, studying the cancer cell phenotype as well as metastasis, replicating TME in organ-on-a-chip and drug development. This technology’s significance and limitations are also addressed, giving readers a comprehensive picture of the ability of the microfluidic platform to advance the field of oncology

Manufacturing Low-Density Particleboards from Wheat Straw and Distiller's Dried Grains with Solubles

Agricultural byproducts like wheat straw and distillers dried grains with solubles (DDGS) have not found a good market in the wood composite industry. Utilizing DDGS for particleboard in addition to using it as feed will add economic value. This study investigated the potential of using DDGS and wheat straw at different proportions in low-density particleboard with phenol-formaldehyde binder. The effect of alkaline pretreatment of DDGS and wheat straw on the properties of board was also studied. Particleboards were tested for physical and mechanical properties, including density, water absorption, thickness swelling, flexural, internal bond, screw withdrawal, and hardness properties. Particleboards with 25% DDGS loading and 75% wheat straw met most of the mechanical properties requirement for low-density particleboards. Alkaline pretreatment did not improve the properties of manufactured particleboards. This study suggests that a higher fiber fraction DDGS and wheat straw can be used in low-density particleboards with reduced synthetic resins