14 research outputs found
The synergic effects of Saffron aqua extract and low frequency electromagnetic field on angiogenesis in chick chorioalantoic membrane
زمینه و هدف: مطالعات in vivo وin vitro بر اثرات ضد سرطانی زعفران تاکید دارند. آنژیوژنز برای تکوین جنین و بسیاری از وقایع فیزیولوژیکی و پاتولوژیک نظیر رشد تومورها، ضروری است. همچنین بسیاری از فرایندهای رشد و نمو تحت تاثیر میدانهای الکترومغناطیسی قرار میگیرند. این مطالعه با هدف بررسی اثرات توام عصاره زعفران و میدان الکترومغناطیسی با فرکانس پایین برآنژیوژنز طراحی شد. روش بررسی: در این پژوهش تجربی آزمایشگاهی تخم مرغ های نطفهدار در 9 گروه شامل گروههای شاهد و شاهد آزمایشگاهی1 و 2، گروههای تجربی 1، 2 و 3 (تیمار با عصاره زعفران با غلظت های 100، 400 و 800 g/mlµ) و گروههای تجربی 4، 5 و 6 (تیمار توام عصاره زعفران همراه میدانالکترومغناطیسی 400 گاؤس) بصورت تصادفی تقسیم شدند. در روز دوم انکوباسیون، در شرایط استریل قسمتی از پوسته تخممرغها برداشته شد و پنجره ای باز ایجاد گردید. در روز هشتم 10 میکرولیتر از غلظت های مختلف عصاره به نمونه ها تیمار گردید. در روز دهم تخممرغها 4 ساعت در معرض میدان الکترومغناطیسی (فرکانس 50 هرتز و شدت 400 گاؤس) قرارگرفتند. در روز دوازدهم از تمامی نمونهها به کمک فتواسترئومیکروسکوپ عکس تهیه و تعداد و طول انشعابات عروقی محل تیمار روی پرده کوریوآلانتوئیک به کمک نرمافزارImage J بررسی گردید. نتایج با استفاده از آزمون های آماری ANOVA و تست تعقیبی Tukey تحلیل شدند. یافته ها: میانگین طول عروق خونی در گروه تجربی 1 نسبت به گروه شاهد کاهش معنی داری نشان نداد (05/0P>) در حالی که در گروههای تجربی 2 و 3 کاهش معنی داری مشاهده شد (001/0
Midazolam-induced learning and memory impairment is modulated by cannabinoid CB1 receptor agonist and antagonist
Background: Memory impairment is a well-known effect of many benzodiazepine compounds which is mediated through their action on gamma-aminobutyric acid type A (GABAA) receptors. On the other hand, cannabinoids can affect learning and memory process through presynaptic modulation of the release of both excitatory glutamate and inhibitory GABA transmitters in brain regions involved in learning and memory. The aim of the present study was to investigate the effect of cannabinoids on memory impairment and long-term potentiation (LTP) reduction properties of the short acting benzodiazepine midazolam.Materials and Methods: One week after insertion of guide cannula by stereotaxic surgery, cannabinoid compounds or midazolam were administered by intracerebroventricular (i.c.v.) injection into lateral ventricle of male rats. Spatial memory task was evaluated using Morris water maze (MWM) test. Electrophysiological evaluation was done by field potential recording of hippocampal neurons in unconscious rats.Results: In MWM test, while i.c.v. administration of AM251 (200 and 500 ng) per se could not change learning and memory function in rats, pretreatment of rats with AM251 (500 ng; i.c.v.) attenuated midazolam-induced memory impairment. In field potential recording, while i.c.v. administration of AM251 (500 ng) and WIN55212-2 (10 μg) did not have any effect on population spike amplitude, pretreatment of rats with both AM251 and WIN55212-2 significantly diminished midazolam-induced PS amplitude reduction in hippocampal neurons.Conclusion: OurOur results suggest the involvement of cannabinoid CB1 receptors in both memory impairment and LTP reduction in hippocampal neurons which was produced by midazolam. This interaction is likely through their effect on both GABAergic and glutamatergic receptors in hippocampus
Effects of Regulated Deficit Irrigation and Superabsorbent Polymer on Fruit Yield and Quality of \u27Granny Smith\u27 Apple
Water resource limitation has caused increasing interest in changing irrigation strategy to improve water use efficiency. In this study, effect of regulated deficit irrigation (RDI) (75 and 50% ETC) in combination with different concentration of superabsorbent polymer (SAP) (0, 100, 200 and 300 g/tree) on fruit yield and quality of \u27Granny Smith\u27 apple was investigated during two consecutive growing seasons. The tree irrigated with 100 ETC was used as control. No significant difference was found between control and RDI and SAP treatments for apple fruit yield. But in the second year of the experiment, apple trees treated with 200 g/tree superabsorbent showed by 15% greater fruit weight. The fruit soluble solid content (SSC) was significantly affected by irrigation regimes and superabsorbent polymer. The highest SSC was found in RDI50 in two years and 200 g/tree superabsorbent in the first year. In the first season, titratable acidity (TA) was significantly affected by SAP levels. The apple trees that received 300 g/tree of SAP, had the highest content of TA in the first season. No significant difference was observed among treatments regarding dry matter percentage and firmness. Total phenolic content of apple fruits was significantly affected by both RDI and SAP treatments in both seasons. In each level of SAP application, by reducing RDI percent total phenolic content increased. The highest phenolic content was found when the trees were treated with 50% RDI with and without SAP treatment
Effects of Regulated Deficit Irrigation and Superabsorbent Polymer on Fruit Yield and Quality of \u27Granny Smith\u27 Apple
Water resource limitation has caused increasing interest in changing irrigation strategy to improve water use efficiency. In this study, effect of regulated deficit irrigation (RDI) (75 and 50% ETC) in combination with different concentration of superabsorbent polymer (SAP) (0, 100, 200 and 300 g/tree) on fruit yield and quality of \u27Granny Smith\u27 apple was investigated during two consecutive growing seasons. The tree irrigated with 100 ETC was used as control. No significant difference was found between control and RDI and SAP treatments for apple fruit yield. But in the second year of the experiment, apple trees treated with 200 g/tree superabsorbent showed by 15% greater fruit weight. The fruit soluble solid content (SSC) was significantly affected by irrigation regimes and superabsorbent polymer. The highest SSC was found in RDI50 in two years and 200 g/tree superabsorbent in the first year. In the first season, titratable acidity (TA) was significantly affected by SAP levels. The apple trees that received 300 g/tree of SAP, had the highest content of TA in the first season. No significant difference was observed among treatments regarding dry matter percentage and firmness. Total phenolic content of apple fruits was significantly affected by both RDI and SAP treatments in both seasons. In each level of SAP application, by reducing RDI percent total phenolic content increased. The highest phenolic content was found when the trees were treated with 50% RDI with and without SAP treatment
Detection of Toxoplasma gondii in Acute and Chronic Phases of Infection in Immunocompromised Patients and Pregnant Women with Real-time PCR Assay Using TaqMan Fluorescent Probe
Background: Toxoplasma gondii, cause severe medical complications in infants and immune-compromised individuals. As using early, sensitive and rapid technique has major in diagnosis of toxoplasmosis, the present study was aimed to detect parasite by using from repetitive element (RE) and B1genes, in blood samples of seropositive immunocompromised patients and pregnant women.
Methods: A total of 110 peripheral blood samples were collected from seropositive cases with anti-T. gondii antibodies, including immunocompromised patients and pregnant women. DNA was extracted by a commercial kit and subjected to TaqMan probe-based real-time PCR assay by using primers and probes specific for RE and B1 genes, separately. The data were analyzed by Kappa test and SPSS-22 software.
Results: In the pregnant women, 17 (68%) and 14 (56%) samples from 25 IgM+/ IgG+ cases and, 7 (25%) and 6 (21.4%) samples from 28 IgG+/IgM- cases were positive by RE and B1 real time PCR, respectively. Likewise, in immunocompromised group, 20 (66.6%) and 17 (56.6%) samples from 30 IgM+/ IgG+ cases and 2 (7.4%) and 2 (7.4%) samples from 27 IgG+/ IgM- cases were positive by RE and B1 real time PCR, respectively.
Conclusion: Probe-based real time PCR assay is a quantitative approach for early diagnosis of T. gondii infection in clinical samples. Moreover, this method can be more appropriate in diagnosis of acute and reactivated toxoplasmosis. In addition our results indicated that RE gene is more sensitive than B1 gene
Utility of blood as the clinical specimen for the diagnosis of ocular toxoplasmosis using uracil DNA glycosylase-supplemented loop-mediated isothermal amplification and real-time polymerase chain reaction assays based on REP-529 sequence and B1 gene
Background: Ocular infection with Toxoplasma gondii is a major preventable cause of blindness, especially in young people. The aim of the present study was to assess detection rate of T. gondii DNA in blood samples of clinically diagnosed of ocular toxoplasmosis using uracil DNA glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP) and real-time quantitative PCR (qPCR) based on REP-529 and B1.
Methods: One hundred and seventeen patients with clinically diagnosed ocular toxoplasmosis (OT) were participated in the study as well as 200 control patients. Peripheral blood samples were assessed using UDG-LAMP and qPCR techniques targeting REP-529 and B1.
Results: Detection limits of qPCR using REP-529 and B1 were estimated as 0.1 and 1 fg of T. gondii genomic DNA, respectively. The limits of detection for UDG-LAMP using REP-529 and B1 were 1 and 100 fg, respectively. In this study, 18 and 16 patients were positive in qPCR using REP-529 and B1, respectively. Based on the results of UDG-LAMP, 15 and 14 patients were positive using REP-529 and B1, respectively. Results of the study on patients with active ocular lesion showed that sensitivity of REP-529 and BI targets included 64 and 63%, respectively using qPCR. Sensitivity of 62 and 61%, were concluded from UDG-LAMP using REP-529 and B1 in the blood cases of active ocular lesion. qPCR was more sensitive than UDG-LAMP for the detection of Toxoplasma gondii DNA in peripheral blood samples of patients with clinically diagnosed toxoplasmic chorioretinitis. Furthermore, the REP-529 included a better detection rate for the diagnosis of ocular toxoplasmosis in blood samples, compared to that the B1 gene did. Moreover, the qPCR and UDG-LAMP specificity assessments have demonstrated no amplifications of DNAs extracted from other microorganisms based on REP-529 and B1.
Conclusions: Data from the current study suggest that qPCR and UDG-LAMP based on the REP-529 are promising diagnostic methods for the diagnosis of ocular toxoplasmosis in blood samples of patients with active chorioretinal lesions
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Intraperitoneal administration of Telmisartan prevents postsurgical adhesion band formation
Background
Angiotensin II receptor blockers (ARBs) have a potential role in reducing inflammation and fibrosis. We have integrated systems and molecular biology approaches to investigate the therapeutic potential of ARBs in preventing postsurgical adhesion band formation.
Material and methods
we have followed the ARRIVE guidelines point by point during experimental studies. Telmisartan (1 and 9 mg/kg), valsartan (1 and 9 mg/kg), and losartan (1 and 10 mg/kg) were administered intraperitoneally in different groups of male albino Wistar rat. After 7 d of treatment, macroscopic evidence and score of fibrotic bands based on scaling methods was performed. Moreover, the anti-inflammatory and antifibrosis effects of telmisartan on reduction of fibrotic bands were investigated by using histopathology, ELISA, and real-time polymerase chain reaction methods.
Results
Telmisartan, but not losartan or valsartan, prevented the frequency as well as the stability of adhesion bands. Telmisartan appears to elicit anti-inflammatory responses by attenuating submucosal edema, suppressing proinflammatory cytokines, decreasing proinflammatory cell infiltration, and inhibiting oxidative stress at the site of peritoneal surgery. We also showed that telmisartan prevents fibrotic adhesion band formation by reducing excessive collagen deposition and suppression of profibrotic genes expression at the peritoneum adhesion tissues.
Conclusions
These results support the potential application of telmisartan in preventing postsurgical adhesion band formation by inhibiting key pathologic responses of inflammation and fibrosis in postsurgery patients