Dampak Pembangunan Perkebunan Karet-rakyat terhadap Kehidupan Petani di Riau

Meaning sense of this paper is to explain and to analyze development process (insmallholder rubber sub sector) that have done by the government in Riau Province. Agriculturalsystem which plantation system is a compatible model with in local people. Afterwards for toexplain reality and to phenomenon analysis and what dynamic happen in that development, soanalysis and it's examine pressured to literacy study and others theory and to use information's.The development problems appear because of the modernization strategy that applied by thegovernment less accommodating local potential's, with the result that local societies think that thedevelopment are not for them. And finally, development programs less popular in the societiesand can not supported by the people, so consequences social change not yet

Human Serum Folate Can Be Measured Using Folate Binding Protein Linked To Enzyme-labeled Protein Ligand Binding Assay (Elplba) AS Well AS Elisa

Background: Folate is an important substance used for purine and pyrimidine nucleotide synthesis. One measurement of folate that already establishes is using ELISA (Enzyme-linked immunosorbent assay) method. Folate binding protein is a protein that can bind folate, therefore it considered can be used as a tool that can replace antibody dependent ELISA method.Objectives: The aim of this research was to create a method for folate measurement in serum called Enzyme-labeled protein ligand binding assay (ELPLBA) by replacing antibody as used in ELISA method with folate binding protein (FBP) that purified from the whey of milk.Methods: The method is tested using 20 serum samples and compared to ELISA. Folate binding protein was purified from bovine\u27s milk using ammonium sulfate up to 90% saturated, DEAE-cellulose anion exchange chromatography and affinity chromatography. SDS-PAGE and western blot were used to establish the protein band of FBP that has molecular weight of ~25-35 kDa. ELPLBA was arranged with stationary phase using aminohexyl-agarose, and folic acid linked on it using carbodiimide.Results: The result show there was no significant difference of folate concentration between ELPLBA (14.804 ± 2.795) and ELISA method (13.859 ± 3.638), p = 0.363.Conclusion: ELPLBA method show similarity for determination of folate in serum which was the same as standard folate measurement (ELISA)