Dynamic Light Scattering from Semidilute Actin Solutions: A Study of Hydrodynamic Screening, Filament Bending Stiffness and the Effect of Tropomyosin/Troponin-Binding

Quasi-elastic light scattering (QELS) is applied to investigate the effect of the tropomyosin/troponin complex (Tm/Tn) on the stiffness of actin filaments. The importance of hydrodynamic screening in semidilute solutions is demonstrated. A new concentration dependent expression for the dynamic structure factor $g(\bm k,t)$ of semiflexible polymers in semidilute solutions is used to analyze the experimental QELS data. A concentration independent value for the bending modulus $\kappa$ is thus obtained. It increases by 50\% as a consequence of Tm/Tn binding in a 7:1:1 molar ratio of actin/Tm/Tn. In addition a new expression for the initial slope of the dynamic structure factor of a semiflexible polymer is used to determine the effective hydrodynamic diameter of the actin filament. Our results confirm the general relevance of the concept of (intrinsic) semiflexibility to polymer dynamics.Comment: 9 pages, RevTeX, 9 figures, all uuencoded gzipe

On Shape Transformations and Shape Fluctuations of Cellular Compartments and Vesicles

We discuss the shape formation and shape transitions of simple bilayer vesicles in context with their role in biology. In the first part several classes of shape changes of vesicles of one lipid component are described and it is shown that these can be explained in terms of the bending energy concept in particular augmented by the bilayer coupling hypothesis. In the second part shape changes and vesicle fission of vesicles composed of membranes of lipid mixtures are reported. These are explained in terms of coupling between local curvature and phase separation

The Glassy Wormlike Chain

We introduce a new model for the dynamics of a wormlike chain in an environment that gives rise to a rough free energy landscape, which we baptise the glassy wormlike chain. It is obtained from the common wormlike chain by an exponential stretching of the relaxation spectrum of its long-wavelength eigenmodes, controlled by a single stretching parameter. Predictions for pertinent observables such as the dynamic structure factor and the microrheological susceptibility exhibit the characteristics of soft glassy rheology and compare favourably with experimental data for reconstituted cytoskeletal networks and live cells. We speculate about the possible microscopic origin of the stretching, implications for the nonlinear rheology, and the potential physiological significance of our results.Comment: 12 pages, 8 figures. Minor correction

Radial distribution function of semiflexible polymers

We calculate the distribution function of the end--to--end distance of a semiflexible polymer with large bending rigidity. This quantity is directly observable in experiments on single semiflexible polymers (e.g., DNA, actin) and relevant to their interpretation. It is also an important starting point for analyzing the behavior of more complex systems such as networks and solutions of semiflexible polymers. To estimate the validity of the obtained analytical expressions, we also determine the distribution function numerically using Monte Carlo simulation and find good quantitative agreement.Comment: RevTeX, 4 pages, 1 figure. Also available at http://www.cip.physik.tu-muenchen.de/tumphy/d/T34/Mitarbeiter/frey.htm

Force-Extension Relation and Plateau Modulus for Wormlike Chains

We derive the linear force-extension relation for a wormlike chain of arbitrary stiffness including entropy elasticity, bending and thermodynamic buckling. From this we infer the plateau modulus $G^0$ of an isotropic entangled solution of wormlike chains. The entanglement length $L_e$ is expressed in terms of the characteristic network parameters for three different scaling regimes in the entangled phase. The entanglement transition and the concentration dependence of $G^0$ are analyzed. Finally we compare our findings with experimental data.Comment: 5 pages, 1 eps-figure, to appear in PR

Polarisation selective magnetic vortex dynamics and core reversal in rotating magnetic fields

A magnetic vortex occurs as an equilibrium configuration in thin ferromagnetic platelets of micron and sub-micron size and is characterised by an in-plane curling magnetisation. At the centre, a magnetic singularity is avoided by an out-of-plane magnetisation core. This core has a gyrotropic excitation mode, which corresponds to a circular motion of the vortex around its equilibrium position, where the rotation sense is determined by the direction of the vortex core magnetisation, its polarisation. Unlike linear fields or spin polarised currents, which excite both polarisation states, an in-plane rotating field can selectively excite one of the polarisation states. Here we report the observation of vortex dynamics in response to rotating magnetic fields, imaged with time-resolved scanning X-ray microscopy. We demonstrate that the rotating field only excites the gyrotropic mode if the rotation sense of the field coincides with the vortex gyration sense and that such a field can selectively reverse the vortex polarisation

Solar Model Uncertainties, MSW Analysis, and Future Solar Neutrino Experiments

Various theoretical uncertainties in the standard solar model and in the Mikheyev-Smirnov-Wolfenstein (MSW) analysis are discussed. It is shown that two methods of estimating the solar neutrino flux uncertainties are equivalent: (a) a simple parametrization of the uncertainties using the core temperature and the nuclear production cross sections; (b) the Monte Carlo method of Bahcall and Ulrich. In the MSW analysis, we emphasize proper treatments of correlation of theoretical uncertainties between flux components and between different detectors, the Earth effect, and multiple solutions in a combined $\chi^2$ procedure. The MSW solutions for various standard and nonstandard solar models are also shown. The MSW predictions of the global solutions for the future solar neutrino experiments are given, emphasizing the measurement of the energy spectrum and the day-night effect in Sudbury Neutrino Observatory and Super-Kamiokande to distinguish the two solutions.Comment: (Revtex 3.0, 43 pages + 26 figures (uuencoded ps files attached), Easy way: ps files of entire text with embedded figures available by anonymous ftp://upenn5.hep.upenn.edu/pub/hata/papers/msw_analysis.u