Non- albicans Candida

The very nature of infectious diseases has undergone profound changes in the past few decades. Fungi once considered as nonpathogenic or less virulent are now recognized as a primary cause of morbidity and mortality in immunocompromised and severely ill patients. Candida spp. are among the most common fungal pathogens. Candida albicans was the predominant cause of candidiasis. However, a shift toward non-albicans Candida species has been recently observed. These non-albicans Candida species demonstrate reduced susceptibility to commonly used antifungal drugs. In the present study, we investigated the prevalence of non-albicans Candida spp. among Candida isolates from various clinical specimens and analysed their virulence factors and antifungal susceptibility profile. A total of 523 Candida spp. were isolated from various clinical specimens. Non-albicans Candida species were the predominant pathogens isolated. Non-albicans Candida species also demonstrated the production of virulence factors once attributed to Candida albicans. Non-albicans Candida demonstrated high resistance to azole group of antifungal agents. Therefore, it can be concluded that non-albicans Candida species have emerged as an important cause of infections. Their isolation from clinical specimen can no longer be ignored as a nonpathogenic isolate nor can it be dismissed as a contaminant

Virulence Factors Contributing to Pathogenicity of Candida tropicalis

The incidence of invasive candidiasis has increased over the past few decades. Although Candida albicans remains by far the most common species encountered, in recent years shift towards non-albicans Candida species like Candida tropicalis is noted. Here in this study we determined the virulence factors and antifungal susceptibility profile of 125 C. tropicalis isolated from various clinical specimens. Biofilm formation was seen in 53 (42.4%) isolates. Coagulase production was noted in 18 (14.4%) isolates. Phospholipase enzyme was the major virulent factor produced by C. tropicalis isolates. A total of 39 biofilm forming isolates showed phospholipase activity. Proteinase activity was demonstrated by 65 (52%) isolates. A total of 38 (30.4%) isolates showed haemolytic activity. Maximum isolates demonstrated resistance to fluconazole. Fluconazole resistance was more common in C. tropicalis isolated from blood cultures. Antifungal resistance was more in isolates possessing the ability to produce phospholipase and biofilm. C. tropicalis exhibit a great degree of variation not only in their pathogenicity but also in their antifungal susceptibility profile. The identification of virulence attributes specific for each species and their correlation with each other will aid in the understanding of the pathogenesis of infection

Catheter Associated Urinary Tract Candida Infections in Intensive Care Unit Patients

Introduction: Health care associated infections (HCAIs) or nosocomial infections have a propensity to strike in the critical care areas. Surveillance of HCAIs, so as to defi ne the magnitude and nature of the problem, is the primary step towards reducing the risk for infection in vulnerable hospitalized patients. The present study was conducted in a rural tertiary care teaching hospital with an aim to determine the rate of catheter associated urinary tract Candida infections in medical intensive care unit patients.Methods: A prospective study was done on catheter associated urinary tract infection (CAUTI). The urine samples were collected and processed following standard microbiological protocols.Results: The overall rate of CAUTI was found to be 1.6 per 1000 catheter days. The rates of catheter associated bacteriuria and candiduria in our health care setup were noted as 1.2 and 0.4 respectively.Conclusion: The present surveillance study helped us to generate institutional data regarding CAUTI in ICU patients with a special reference to candiduria. In our institute, the incidence of candiduria was low.</p