1,266 research outputs found
... Pour le peuple d'Asie mineure...Â
BrÚve évocation de l'actualité du roman de Dido Sotiriou / Matoména homata / D'un jardin d'Anatolie, Paris, EFR, 1965, et de son importance pour comprendre l'héritage de la cohabitation des peuples grec et turc dans l'Asie mineure d'avant 1921 et leur séparation
... Pour le peuple d'Asie mineure...Â
BrÚve évocation de l'actualité du roman de Dido Sotiriou / Matoména homata / D'un jardin d'Anatolie, Paris, EFR, 1965, et de son importance pour comprendre l'héritage de la cohabitation des peuples grec et turc dans l'Asie mineure d'avant 1921 et leur séparation
Coupling Tumor Necrosis Factor-α with αV Integrin Ligands Improves Its Antineoplastic Activity
Despite the impressive results obtained in animal models, the clinical use of tumor necrosis factor-α (TNF) as an anticancer drug is limited by severe toxicity. We have shown previously that targeted delivery of TNF to aminopeptidase N (CD13), a marker of angiogenic vessels, improved the therapeutic index of this cytokine in tumor-bearing mice. To assess whether the vascular-targeting approach could be extended to other markers of tumor blood vessels, in this work, we have fused TNF with the ACDCRGDCFCG peptide, a ligand of αV integrins by recombinant DNA technology. We have found that subnanogram doses of this conjugate are sufficient to induce antitumor effects in tumor-bearing mice when combined with melphalan, a chemotherapeutic drug. Cell adhesion assays and competitive binding experiments with anti-integrin antibodies showed that the Arg-Gly-Asp moiety interacts with cell adhesion receptors, including αVÎČ3 integrin, as originally postulated. In addition, ACGDRGDCFCG-mouse TNF conjugate induced cytotoxic effects in standard cytolytic assays, implying that ACGDRGDCFCG-mouse TNF conjugate can also bind TNF receptors and trigger death signals. These results indicate that coupling TNF with αV integrin ligands improves its antineoplastic activity and supports the concept that vascular targeting is a strategy potentially applicable to different endothelial markers, not limited to CD13
Potential Clinical Applications of the Postbiotic Butyrate in Human Skin Diseases
Human skin is the largest organ and the most external interface between the environment and the body. Vast communities of viruses, bacteria, archaea, fungi, and mites, collectively named the skin microbiome (SM), cover the skin surface and connected structures. Skin-resident microorganisms contribute to the establishment of cutaneous homeostasis and can modulate host inflammatory responses. Imbalances in the SM structure and function (dysbiosis) are associated with several skin conditions. Therefore, novel target for the skincare field could be represented by strategies, which restore or preserve the SM natural/individual balance. Several of the beneficial effects exerted by the SM are aroused by the microbial metabolite butyrate. Since butyrate exerts a pivotal role in preserving skin health, it could be used as a postbiotic strategy for preventing or treating skin diseases. Herein, we describe and share perspectives of the potential clinical applications of therapeutic strategies using the postbiotic butyrate against human skin disease
Boosting Interleukin-12 Antitumor Activity and Synergism with Immunotherapy by Targeted Delivery with isoDGR-Tagged Nanogold.
AbstractThe clinical use of interleukinâ12 (IL12), a cytokine endowed with potent immunotherapeutic anticancer activity, is limited by systemic toxicity. The hypothesis is addressed that gold nanoparticles tagged with a tumorâhoming peptide containing isoDGR, an αvÎČ3âintegrin binding motif, can be exploited for delivering IL12 to tumors and improving its therapeutic index. To this aim, gold nanospheres are functionalized with the headâtoâtail cyclizedâpeptide CGisoDGRG (Iso1) and murine IL12. The resulting nanodrug (Iso1/Au/IL12) is monodispersed, stable, and bifunctional in terms of αvÎČ3 and IL12âreceptor recognition. Lowâdose Iso1/Au/IL12, equivalent to 18â75 pg of IL12, induces antitumor effects in murine models of fibrosarcomas and mammary adenocarcinomas, with no evidence of toxicity. Equivalent doses of Au/IL12 (a nanodrug lacking Iso1) fail to delay tumor growth, whereas 15 000 pg of free IL12 is necessary to achieve similar effects. Iso1/Au/IL12 significantly increases tumor infiltration by innate immune cells, such as NK and iNKT cells, monocytes, and neutrophils. NK cell depletion completely inhibits its antitumor effects. Lowâdose Iso1/Au/IL12 can also increase the therapeutic efficacy of adoptive Tâcell therapy in mice with autochthonous prostate cancer. These findings indicate that coupling IL12 to isoDGRâtagged nanogold is a valid strategy for enhancing its therapeutic index and sustaining adoptive Tâcell therapy
pMineR: An Innovative R Library for Performing Process Mining in Medicine
Process Mining is an emerging discipline investigating tasks related with the automated identification of process models, given realworld
data (Process Discovery). The analysis of such models can provide useful insights to domain experts. In addition, models of processes can
be used to test if a given process complies (Conformance Checking) with specifications. For these capabilities, Process Mining is gaining importance and attention in healthcare.
In this paper we introduce pMineR, an R library specifically designed for performing Process Mining in the medical domain, and supporting
human experts by presenting processes in a human-readable way
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