How do ectotherms perform in cold environments? Physiological and life-history traits in an Andean viviparous lizard

Material complementario: https://www.frontiersin.org/articles/10.3389/fevo.2022.974968/full#supplementary-materialBoth the mean and the variation in environmental temperature are increasing globally. Indeed, the predicted increases in temperature range from 2 to 4°C in the next 50 years. Ectotherms control body temperature by means of behavior selecting microsites with different temperatures, which makes them more susceptible to changes in climate. Nevertheless, lizards living in high mountain environments have developed several mechanisms to inhabit and colonize variable environments with extreme temperatures. These mechanisms include a high metabolism to be active at lower temperatures and viviparity to improve embryonic development. Despite behavioral thermoregulation acting as a buffer to changes in environmental temperature, other traits such as lifehistory traits may be less flexible. Consequently, in an attempt to understand how lizards cope with harsh habitats, we evaluated some physiological traits and responses of females of Liolaemus bellii from two contrasting slope sites with differences in environmental temperature and humidity, but at the same altitude in the southern Andes range. We collected pregnant females from opposite slopes and maintained them until parturition in a commongarden experiment. Females from the south-facing slope (S-slope) had higher preferred body temperature (Tpref) values before and after parturition and exhibited higher daily energy expenditure before parturition. Nevertheless, no difference in Tpref was shown by their offspring, suggesting a developmental plastic response or adaptation to lower environmental temperature. For instance, the higher metabolism during pregnancy could be associated with a shorter activity period on the snowy S-slope. Additionally, females from the S-slope had larger kidneys and gave birth later than N-slope females, likely due to developmental plasticity or genetic differentiation. How fixed these traits are, in individuals from the contrasting slopes, will determine the response capacity of the L. bellii population to climate change

A reciprocal transplant experiment confirmed mite-resistance in a honey bee population from Uruguay

In the past few years there has been an increasing interest for the study of honey bee populations that are naturally resistant to the ectoparasitic mite Varroa destructor, aiming to identify the mechanisms that allow the bees to limit the reproduction of the mite. In eastern Uruguay there are still bees resistant to mites that survive without acaricides. In order to determine if the differential resistance to V. destructor was maintained in other environments, a reciprocal transplant experiment was performed between the mite-resistant bee colonies and the mite-susceptible bee colonies from the east and the west of the country, respectively, infesting bees with local mites. In both regions, the mite-resistant colonies expressed a higher hygienic behavior and presented a higher phoretic mites/reproductive mites and mites in drone cells/mites in worker cells ratio than the mite-susceptible colonies. All the mite-susceptible colonies died during fall–winter, while a considerable number of mite-resistant colonies survived until spring, especially in the east of the country. This study shows that the bees in the east of the country maintain in good measure the resistance to V. destructor in other regions and leaves open the possibility that the mites of the two populations have biases in the reproductive behavior.INIA: PF10.ANII:POS_NAC_2013_1_1225

Rapid within- and transgenerational changes in thermal tolerance and fitness in variable thermal landscapes

Phenotypic plasticity may increase the performance and fitness and allow organisms to cope with variable environmental conditions. We studied within-generation plasticity and transgenerational effects of thermal conditions on temperature tolerance and demographic parameters in Drosophila melanogaster. We employed a fully factorial design, in which both parental (P) and offspring generations (F1) were reared in a constant or a variable thermal environment. Thermal variability during ontogeny increased heat tolerance in P, but with demographic cost as this treatment resulted in substantially lower survival, fecundity, and net reproductive rate. The adverse effects of thermal variability (V) on demographic parameters were less drastic in flies from the F1, which exhibited higher net reproductive rates than their parents. These compensatory responses could not totally overcome the challenges of the thermally variable regime, contrasting with the offspring of flies raised in a constant temperature (C) that showed no reduction in fitness with thermal variation. Thus, the parental thermal environment had effects on thermal tolerance and demographic parameters in fruit fly. These results demonstrate how transgenerational effects of environmental conditions on heat tolerance, as well as their potential costs on other fitness components, can have a major impact on populations’ resilience to warming temperatures and more frequent thermal extremes

Increasing winter maximal metabolic rate improves intrawinter survival in small birds

Small resident bird species living at northern latitudes increase their metabolism in winter, and this is widely assumed to improve their chances of survival. However, the relationship between winter metabolic performance and survival has yet to be demonstrated. Using capture-mark-recapture, we followed a population of free-living black-capped chickadees (Poecile atricapil-lus) over 3 yr and evaluated their survival probability within and among winters. We also measured the size-independent body mass (Ms), hematocrit (Hct), basal metabolic rate (BMR), and maximal thermogenic capacity (Msum) and investigated how these parameters influenced survival within and among winters. Results showed that survival probability was high and constant both within (0.92) and among (0.96) winters. They also showed that while Ms, Hct, and BMR had no significant influence, sur- vival was positively related to Msum—following a sigmoid re-lationship——within but not among winter. Birds expressing an Msum below 1.26 W (i.e., similar to summer levels) had a !50% chance of survival, while birds with an Msum above 1.35W had at least a 90% chance of surviving through the winter. Our data therefore suggest that black-capped chickadees that are either too slow or unable to adjust their phenotype from summer to winter have little chances of survival and thus that seasonal upregulation of metabolic performance is highly beneficial. This study is the first to document in an avian system the relationship between thermogenic capacity and winter survival, a proxy of fitnes

Thermal performance of the Chagas disease vector, Triatoma infestans, under thermal variability.

Vector-borne diseases (VBD) are particularly susceptible to climate change because most of the diseases' vectors are ectotherms, which themselves are susceptible to thermal changes. The Chagas disease is one neglected tropical disease caused by the protozoan parasite, Trypanosoma cruzi. One of the main vectors of the Chagas disease in South America is Triatoma infestans, a species traditionally considered to be restricted to domestic or peridomestic habitats, but sylvatic foci have also been described along its distribution. The infestation of wild individuals, together with the projections of environmental changes due to global warming, urge the need to understand the relationship between temperature and the vector's performance. Here, we evaluated the impact of temperature variability on the thermal response of T. infestans. We acclimated individuals to six thermal treatments for five weeks to then estimate their thermal performance curves (TPCs) by measuring the walking speed of the individuals. We found that the TPCs varied with thermal acclimation and body mass. Individuals acclimated to a low and variable ambient temperature (18°C ± 5°C) exhibited lower performances than those individuals acclimated to an optimal temperature (27°C ± 0°C); while those individuals acclimated to a low but constant temperature (18°C ± 0°C) did not differ in their maximal performance from those at an optimal temperature. Additionally, thermal variability (i.e., ± 5°C) at a high temperature (30°C) increased performance. These results evidenced the plastic response of T. infestans to thermal acclimation. This plastic response and the non-linear effect of thermal variability on the performance of T. infestans posit challenges when predicting changes in the vector's distribution range under climate change

Differential responses to thermal variation between fitness metrics

Temperature is a major factor affecting population abundance and individual performance. Net reproductive rate (R0) and intrinsic rate of increase (r) differ in their response to different temperature regimes, and much of the difference is mediated by generation time (Tg). Here, we evaluate the effects of thermal mean and variability on R0, r and Tg, at four population densities in Drosophila melanogaster. The results show that R 0, r and Tg present differential responses to thermal variation. Although temperature effects on R0 and Tg are non-linear, r response was negligible. R0 and Tg comprise a generational time scale, while r is at a chronological time scale. Thus, we argue that individuals growing under different thermal environments perform similarly on a chronological scale, but differently on a generational scale

Diet of four annual killifishes: an intra and interspecific comparison

We examined the diet of 4 annual fishes, Austrolebias viarius, Austrolebias cheradophilus, Austrolebias luteoflammulatus and Cynopoecilus melanotaenia inhabiting temporal ponds of southeastern Uruguay, by analysis of stomach contents. Fishes were captured from fifty ephemeral ponds of Castillos Lagoon basin, in the region of the Humedales del Este. We identified 13099 individual prey items extracted from 669 stomachs of the four captured species. In the studied system, annual killifishes represents the most abundant and conspicuous top predators. Killifishes are generalist key predators at the ephemeral ponds of the studied system, consuming mostly aquatic items. Zooplancton represented the bulk of the diet in the four analyzed species, followed by eggs, algae and diatoms. Insects are the next group in prey number, as follows: Diptera larvae (especially Chironomidae and Cullicidae), Ephemeroptera (especially Betidae), and coleopteran larvae (especially Dytiscidae). Acari are also important prey in number. The four fish species differ in diet composition and in diet richness. A general pattern of differences in diet richness among killifish species and demographic groups could be related to variations in body sizes. As top predators annual killifishes are an important component of the temporal pond ecosystems. Understanding the natural history of this species and their communities is necessary in order to conserve them

Rapid within‐ and transgenerational changes in thermal tolerance and fitness in variable thermal landscapes

Phenotypic plasticity may increase the performance and fitness and allow organisms to cope with variable environmental conditions. We studied within-generation plasticity and transgenerational effects of thermal conditions on temperature tolerance and demographic parameters in Drosophila melanogaster. We employed a fully factorial design, in which both parental (P) and offspring generations (F1) were reared in a constant or a variable thermal environment. Thermal variability during ontogeny increased heat tolerance in P, but with demographic cost as this treatment resulted in substantially lower survival, fecundity, and net reproductive rate. The adverse effects of thermal variability (V) on demographic parameters were less drastic in flies from the F1, which exhibited higher net reproductive rates than their parents. These compensatory responses could not totally overcome the challenges of the thermally variable regime, contrasting with the offspring of flies raised in a constant temperature (C) that showed no reduction in fitness with thermal variation. Thus, the parental thermal environment had effects on thermal tolerance and demographic parameters in fruit fly. These results demonstrate how transgenerational effects of environmental conditions on heat tolerance, as well as their potential costs on other fitness components, can have a major impact on populations’ resilience to warming temperatures and more frequent thermal extremes