Characterization of TbPH1, a kinetoplastid-specific pleckstrin homology domain containing kinesin-like protein

The aim of this master thesis was the investigation of the uncharacterized protein TbPH1, by in silico studies, determining effects of its knock-down, studying the effect of a knock-down on the cell cycle, examining its cellular localization, and finding out about possible complexes and interaction-partners

The Localization of the Mitochondrial Proteins MRP1, KREL2 and LSU1 of Trypanosoma brucei

The aim of this thesis was to investigate the localization of the mitochondrial proteins MRP1, KREL2 and LSU1 of Trypanosoma brucei. Furthermore, the proper functioning of the eYFP-tagged protein MRP1 was investigated

Effect of 24 hour doxycycline exposure on the viability of procyclic stage <i>T</i>. <i>brucei</i>.

<p>Data points represent the mean cell viability ± standard error of the mean (SEM) (<i>n</i> = 4), as measured by the Alamar Blue fluorescent dye assay. <i>X</i>-axis, μg/ml doxycycline (log scale); <i>y</i>-axis Alamar Blue fluorescence intensity in arbitrary units; doxycycline EC₅₀ value calculated from curve given on lower left. Red arrows indicate points corresponding to 1 and 100 μg/ml concentrations on the <i>x</i>-axis. The assay was performed as previously described [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005492#ppat.1005492.ref019" target="_blank">19</a>].</p

The ribosomal small subunit rRNA loops containing H31 and H34, as well as H27, double-stranded helices from bacteria (black) plus the mitochondria of mammals (green), plants (blue) and trypanosomes (red).

<p>Grey shading highlights the location of H31 and H34 in the SSU rRNAs bearing these motifs, as well as their absence in the same region of the trypanosomatid SSU rRNA. Helix H30, which is conserved throughout all the depicted rRNAs, is also indicated as a reference point. Adapted from [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005492#ppat.1005492.ref008" target="_blank">8</a>] and [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005492#ppat.1005492.ref009" target="_blank">9</a>].</p