Parentage verification and identity test of Ghezel sheep using microsatillate markers

The Ghezel sheep is a fat tail high weight Iranian breed which is raised in the North-west of Iran. To design an efficient improvement program and genetic evaluation system for this indigenous breed, accurate estimates of the population genetic parameters is per-required and all pedigrees and relationships should be correctly recorded. Otherwise, it can produce biased evaluations when pedigrees contain errors and procedures utilize information from relatives. The pedigree and genotype data of Ghezel sheep were examined for errors. Parentage control has been performed by amplification of microsatellites. Mean heterozygosities, mean polymorphism index content (PIC) and mean number of alleles per loci were 0.50, 0.43 and 3.71, respectively. Mendelian errors were found following the pedigree corrections. Alleles at the following seven microsatellite loci were identified: BM4307, CSSM004, BM415, RM029, INRA49, BM3205 and OarFCB5. The pedigree was considered incorrect in 6 (12%) out of all the evaluated progeny, as their genotype did not match their parents. The present findings attest to the usefulness of the investigated microsatellites for parentage control in Ghezel sheep.Key words: Ghezel sheep, microsatellites, genotyping errors, progeny test

Inter simple sequence repeat (ISSR) markers as reproducible and specific tools for genetic diversity analysis of rose species

Rose is one of the most important cultivated ornamental plants in the world. A molecular approach using inter-simple sequence repeat (ISSR) markers was applied to seven species of Rosa. To obtain clear and reproducible bands on 2% agarose gels, 9 ISSR primers and 5 parameters (annealing temperature, DNA concentrations, primer concentrations, Taq DNA polymerase and MgCl2 concentrations) were screened. The resolution of six ISSR markers was performed, with optimal annealing temperature (Ta) varying from 45 to 50°C. A total of 66 DNA fragments were amplified, of which 50 were polymorphic. The optimal conditions for ISSR system were determined as follows: MgCl2 concentration was 2 mM, the quantity of Taq DNA polymerase 1 U, template DNA 30 ng and the concentration of primer was 1 μM and the reaction program was: initial denaturation for 5 min at 94°C, 35 cycles of denaturation for 30 s at 94°C, annealing for 45 s at specific annealing temperature for each primer, extension for 2 min at 72°C and a final 10 min extension at 72°C.Key words: Inter-simple sequence repeat marker, rose species, genetic diversity, optimization

Exploring the Impact of Heat Stress on Oocyte Maturation and Embryo Development in Dairy Cattle Using a Controlled and Defined Culture Medium supplemented with Vitamins E, C, and Coenzyme Q10

The data presents a raw information of the experiment performed on the effects of vit E, vit C, and coenzyme Q10 on IVM and IVF of dairy cattle under summer heat stress.THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV