3,6-Di(pyridin-2-yl)-1,2,4,5-s-tetrazine capped-gold nanoparticles as an efficient antibacterialagent against gram-positive Bacillus subtilis

1627-1631In current work, we present 3,6-di(pyridin-2-yl)-1,2,4,5-s-tetrazine (pytz) capped gold nanoparticles (TzAuNPs) as an potent antibacterial agent against Gram-positive bacteria. The antibacterial properties of TzAuNPs have been studied using Gram-positive Bacillus subtilis (ATCC 11774) by the plate count method and TzAuNPs is found to be highly effective against the strain. Furthermore, TzAuNPs also show significantly better antimicrobial activity against the bacterial strain when compared to pytz. The effect of TzAuNPs on the bacterial cell wall is also investigated by TEM analysis. This new antimicrobial material could be promising in future for the treatment infectious diseases caused by Gram-positive bacteria

Conserved Activity of Reassociated Homotetrameric Protein Subunits Released from Mesoporous Silica Nanoparticles

Mesoporous silica nanoparticles (MSN) with enlarged pores were prepared and characterized, and reversibly dissociated subunits of concanavalin A were entrapped in the mesopores, as shown by multiple biochemical and material characterizations. When loaded in the MSN, we demonstrated protein stability from proteases and, upon release, the subunits reassociated into active proteins shown through mannose binding and <i>o</i>-phthalaldehyde fluorescence. We have demonstrated a versatile and facile method to load homomeric proteins into MSN with potential applications in enhancing the delivery of large therapeutic proteins