Effect of extracted garlic powder ingestion for two months on exercise-induced immunological responses

Introduction. Exhaustive exercise is associated with an increased risk of upper respiratory tract infection. Previously, allicin supplementation has been reported to reduce the incidence of common cold symptoms and production of exercise-induced interleukin (IL)-6. However, it is not clear if daily ingestion of the edible portion of whole garlic (Allium sativum) alters the exercise-induced immunological response. The present study investigated the effects of extracted garlic powder ingestion for 2 months on immune cell counts, natural killer cell activity (NKCA), as well as changes in cytokines, cortisol, and lactic acid in response to high-intensity cycling exercise. Methods. The present study employed a before-after study design. Six sedentary male participants (age, 22.0±0.3 years) consumed extracted garlic powder for 2 months, and underwent 45 minutes of cycling exercise at 80% of the heart rate reserve once before and once after the supplementation period. A thousand milligrams of extracted garlic powder, comparable to 6 g (1 clove) of raw garlic, was ingested every day. Blood samples were obtained at the following five time points: before exercise, 0 min, 30 min, 60 min, and 120 min after exercise. We measured NKCA, leukocyte counts, neutrophil counts, lymphocyte counts, as well as levels of serum IL-6, IL-10, cortisol, and lactic acid. Repeated measures ANOVA was used for statistical analyses. When interaction effects were significant, measurement values at the various time points were compared between pre- and post-supplementation period using the paired t-test. Changes were deemed statistically significant when p\u3c0.05. Results. We observed no significant difference in pre-exercise measurements between pre- and post-supplementation periods. In addition, we found no significant interaction effect for leukocytes, neutrophils, NKCA, IL-10, and cortisol. However, we did identify a significant interaction effect for lymphocytes, IL-6, and lactic acid (p=0.033, p=0.030, and p\u3c0.001, respectively). Lymphocyte counts were significantly lower post-supplementation relative to pre-supplementation immediately after exercise (p=0.014). In addition, IL-6 was significantly lower post-supplementation relative to pre-supplementation immediately and 30 minutes after exercise (p=0.015 and p=0.018, respectively). Lactic acid levels were significantly lower post-supplementation relative to pre-supplementation immediately after exercise (p=0.018). Conclusions. The extracted garlic powder did not significantly influence exercise-induced responses by leukocytes, neutrophils, NKCA, IL-10, or cortisol. However, exercise-induced responses by lymphocytes, IL-6, and lactic acid were suppressed after ingestion of extracted garlic powder. Thus, daily ingestion of the edible portion of whole garlic may suppress exercise-induced immunological responses and lactic acid levels

ジョシ シンタイソウ センシュ ニ オケル チュウショク ノ エネルギーリョウ ガ エネルギー セッシュリョウ ヤ タイシボウリツ ニ オヨボス エイキョウ

本研究は減量期の女子新体操選手に対する5週間の昼食提供が1日の総エネルギー摂取量や体重,体脂肪率に及ぼす影響を検討することを目的とした。対象者は16名(年齢19.5±1.0歳)であり,昼食のエネルギー量は561±74kcal,たんぱく質量は29.2±4.3gであった。身体特性は昼食提供前(提供前)と昼食提供後(提供後)の計2回,栄養素等摂取量は提供前と昼食提供中(提供中)を調べた。統計解析は対応のあるt-検定,Wilcoxonの符号付き順位和検定,McNemar検定,Pearsonの積率相関係数,Spearmanの順位相関係数を用いた。昼食を提供した結果,総エネルギー摂取量,食事別エネルギー摂取量および総エネルギー消費量には提供前と提供中で有意な違いがみられず,昼食エネルギー摂取割合は提供前(23.3±10.4%)に比べて提供中(36.5±15.3%)が有意に増加した(p=0.005)。提供前と提供後で除脂肪体重には有意差がみられなかったものの,体重(48.4±3.2kgから47.6±3.2kg, p=0.001)および体脂肪率(13.4±2.0%から12.3±1.6%,p=0.008)は有意に低下した。しかし,体脂肪率の変化量は食事別エネルギー摂取割合の変化量との間に有意な関連がみられなかった。以上より,昼食提供は1日のエネルギー摂取量,食事別エネルギー摂取量に影響を与えず,昼食エネルギー摂取割合を増加させ,体脂肪率を減少させることが示された。体脂肪率と各因子間には有意な関連がみられなかったが,昼食を摂ることは減量期の選手にとって重要である可能性が示唆された。The present study aimed to examine the effect of lunch on total energy intake, body weight, and body fat percentage during weight reduction periods. Sixteen female rhythmic gymnasts (mean age, 19.5±1.0 years) participated in this study. School lunch was provided (561±74kcal, 29.2±4.3g protein) for five weeks. Physical characteristics were assessed twice, before (Before) and after (After) the lunch-providing period. Dietary intake was assessed twice, Before and during the lunch-providing period. The paired t-test, Wilcoxon signed-rank test, McNemar test, Pearson\u27s product moment correlation coefficient, and Spearman\u27s rank correlation coefficient were used for the statistical analyses. After the lunch-providing period, no significant differences were found in total energy intake, each meal energy intake, and total energy expenditure relative to Before and during the lunch providing-period, but a significant increase was found in the ratio of lunch to total energy intake (23.3±10.4% to 36.5±15.3%, p=0.005). Moreover, significant decreases in body weight (48.4±3.2kg to 47.6±3.2kg, p=0.001) and body fat percentage (13.4±2.0% to 12.3±1.6%, p=0.008), but not lean body mass, were observed relative to Before. However, changes in body fat percentage were not correlated with changes in the ratio of each meal energy intake. From these results, providing lunch does not affect total energy intake or each meal energy intake, but increases the ratio of lunch to total energy intake and decreases body fat percentage. The body fat percentage was not correlated with each factor. Our findings suggest the importance of providing lunch to female rhythmic gymnasts during weight reduction periods

ムシュウ ニンニク 2カゲツカン ノ セッシュ ハ ウンドウ ユウハツセイ IL-6 ジョウショウ ヲ ヨクセイ スル：ゼンゴ ヒカク シケン

ニンニクに含まれるアリシンはヒトで運動誘発性インターロイキン（IL）6産生を抑制することが報告されている。しかし，アリシンは特有の強い臭気を持つため，実生活を考えると日常的に使用することが難しい。我々はそこで，無臭ニンニク（odorless garlic ; OG）2か月間摂取が運動誘発性IL-6産生と他の免疫応答を減弱する可能性について検討することを目的とした。日頃からあまり活動的でない6名の健康な男性（22.0±0.7歳）を対象に一日当たり1 gのOGを2か月間にわたって摂取させた。研究での運動負荷条件は推定最大心拍数の80％強度の自転車運動とし，45分間実施した。運動負荷は，実験開始前（control群 ; CON-Ex）とニンニク摂取2か月後（odorless garlic supplementation and exercise群 ; OG-Ex）に実施した。血液サンプルは運動負荷を行う前，運動負荷直後（0 min），30分後，60分後に採取し，IL-6，IL-10，白血球数，好中球数，リンパ球数，Natural Killer Cell Activity （NKCA），コルチゾールを測定した。運動負荷前から運動負荷60分後までの上昇曲線下面積（iAUC）を算出し，統計解析には繰り返しのある二元配置分散分析（ANOVA）およびHolm’s methodを用いた対応のあるt検定を行った。運動負荷前の生化学値にはCON-ExとOG-Exの間に有意な差は見られなかった。運動負荷後の白血球数，好中球数，リンパ球数，NKCA，IL-10，およびコルチゾールに交互作用は見られなかったものの，IL-6には交互作用が見られた（p＝0.011）。OG-Ex 群の運動誘発性IL-6 iAUC値はCON-Ex群に比べて有意な低下がみられた（CON-Ex, 82.5±12.2 min･pg/mL ; OG-Ex, 55.5±14.2 min･pg/mL ; p＝0.018）。今回の結果は2か月間のOG摂取が運動誘発性IL-6上昇を抑制するという仮説を支持する結果となった。しかしながら，OG摂取は他の免疫指標に影響を及ぼすことはなかった。OG摂取が運動誘発性の免疫機能に及ぼす影響については，今後のさらなる研究が必要である。Allicin, which is a primary compound in crushed garlic, inhibits exercise-induced interleukin (IL)-6 production in humans. However, the daily use of crushed garlic or stabilized allicin is not practical due to the strong odor of allicin. We therefore investigated the possibility that odorless garlic (OG) supplementation for 2 months attenuates exercise-induced IL-6 and other immunological responses. Six healthy untrained men (aged 22.0±0.7 years) consumed 1 g of OG per day for 2 months. Participants underwent two 45-min periods of acute cycling exercise at 80% intensity of heart rate reserve (HRR). The first period was at baseline (control exercise ; CON-Ex) and the second after 2 months of OG supplementation (odorless garlic supplementation and exercise ; OG-Ex). To assess levels of IL-6, IL-10, leukocytes, neutrophils, lymphocytes, Natural Killer Cell Activity (NKCA) and cortisol, blood samples were collected before and immediately (0 min), and 30 and 60 min after each 45-min period of exercise. Two-way repeated measures analysis of variance (ANOVA) and paired t-test with Holm’s method were performed, and the incremental area under the curve (iAUC) from before to 60 min after exercise was calculated. No significant differ­ences in the pre-exercise levels of biochemical indices were observed between the CON-Ex and OG-Ex assessments. No significant interaction effects were observed in exercise-induced changes in leukocytes, neutrophils, lymphocytes, NKCA, IL-10, or cortisol. A significant interaction effect was observed in exercise-induced changes in IL-6 (p＝0.011). OG consumption also significantly decreased the iAUC of IL-6 (CON-Ex, 82.5±12.2 min･pg/mL ; OG-Ex, 55.5±14.2 min･pg/mL ; p＝0.018). Our findings support the hypothesis that two months of OG supplementation attenuates exercise-induced increases in IL-6 levels among untrained men. However, OG did not exert a similar effect on other immunological parameters. Further study is required to clarify the effect of OG on exercise-induced activation of the immune system

Validity of a Semi-Quantitative Food Frequency Questionnaire for Collegiate Athletes

Background: Food frequency questionnaires (FFQs) have been developed and validated for various populations. To our knowledge, however, no FFQ has been validated for young athletes. Here, we investigated whether an FFQ that was developed and validated to estimate dietary intake in middle-aged persons was also valid for estimating that in young athletes. Methods: We applied an FFQ that had been developed for the Japan Public Health Center-based Prospective Cohort Study with modification to the duration of recollection. A total of 156 participants (92 males) completed the FFQ and a 3-day non-consecutive 24-hour dietary recall (24hDR). Validity of the mean estimates was evaluated by calculating the percentage differences between the 24hDR and FFQ. Ranking estimation was validated using Spearman’s correlation coefficient (CC), and the degree of miscategorization was determined by joint classification. Results: The FFQ underestimated energy intake by approximately 10% for both males and females. For 35 nutrients, the median (range) deattenuated CC was 0.30 (0.10 to 0.57) for males and 0.32 (−0.08 to 0.62) for females. For 19 food groups, the median (range) deattenuated CC was 0.32 (0.17 to 0.72) for males and 0.34 (−0.11 to 0.58) for females. For both nutrient and food group intakes, cross-classification analysis indicated extreme miscategorization rates of 3% to 5%. Conclusions: An FFQ developed and validated for middle-aged persons had comparable validity among young athletes. This FFQ might be useful for assessing habitual dietary intake in collegiate athletes, especially for calcium, vitamin C, vegetables, fruits, and milk and dairy products

The Validity and Reproducibility of Dietary Non-enzymatic Antioxidant Capacity Estimated by Self-administered Food Frequency Questionnaires

Background: High dietary non-enzymatic antioxidant capacity (NEAC) has been inversely related to the incidence of degenerative diseases. However, few studies have investigated the validity and reproducibility of dietary NEAC estimated from a food frequency questionnaire (FFQ). We assessed the validity and reproducibility of FFQ-based dietary NEAC against a dietary record (DR). Methods: Participants were 244 men and 253 women who completed a 28-day DR and FFQs. NEAC for each food item was estimated according to available databases of antioxidant capacity, as measured by ferric reducing-antioxidant power (FRAP), oxygen radical absorbance capacity (ORAC), and total radical-trapping antioxidant parameter (TRAP). Using Spearman’s rank correlation coefficients (CCs), we assessed the validity for dietary NEACs from a 28-day DR and a FFQ, and the reproducibility for them from two FFQs administered at a 1-year interval. Additionally, joint classification and the Bland-Altman method were applied to assess agreement between the two methods. Results: Regarding validation, deattenuated CCs for the energy-adjusted overall dietary NEACs between FFQ and DR for FRAP, ORAC, and TRAP were 0.52, 0.54, and 0.52, respectively, for all subjects. Extreme miscategorization rates by joint classification analysis were 2% for FRAP and ORAC and 1% for TRAP. Regarding reproducibility, CCs between the energy-adjusted dietary NEACs from two FFQs were 0.64 for FRAP and 0.65 for ORAC and TRAP. Conclusion: The validity and reproducibility of dietary NEAC of total food from the FFQ were moderate. Estimations of dietary NEAC using FFQ would be useful in studying disease relationships by categorizing habitual dietary NEAC