4,028 research outputs found
unfixed and fixed human chromosomes show different staining patterns after restriction endonuclease digestion
Restriction endonucleases (REs) have been widely used to produce banding patterns on chromosomes, but it remains uncertain to what extent the patterns are due to the sequence specificity of the enzymes, and to what extent chromatin structure influences the pattern of digestion. To throw light on this question, we have digested with restriction endonucleases unfixed chromosomes prepared in two different ways (isolated, and whole metaphase cells spread with a cytocentrifuge) and compared the results with those obtained on conventionally fixed chromosomes. Unfixed isolated chromosomes are easily destroyed by REs; after fixation with cold methanol, which produced minimal alteration to the chromatin structure, the chromosomes are resistant to the action of REs, and conventional methanol-acetic acid fixation is required to permit the induction of banding patterns by REs. Unfixed cytocentrifuge preparations, in which the chromosomes are still surrounded by cytoplasm, are much more resistant to the action of REs, and again banding patterns were only induced after methanol-acetic acid fixation. We conclude that the action of restriction endonucleases on chromosomes is strongly influenced by chromatin organisation, and that methanol-acetic acid fixation is required to permit the induction of conventional banding patterns on chromosomes
Maximum likelihood estimates of pairwise rearrangement distances
Accurate estimation of evolutionary distances between taxa is important for
many phylogenetic reconstruction methods. In the case of bacteria, distances
can be estimated using a range of different evolutionary models, from single
nucleotide polymorphisms to large-scale genome rearrangements. In the case of
sequence evolution models (such as the Jukes-Cantor model and associated
metric) have been used to correct pairwise distances. Similar correction
methods for genome rearrangement processes are required to improve inference.
Current attempts at correction fall into 3 categories: Empirical computational
studies, Bayesian/MCMC approaches, and combinatorial approaches. Here we
introduce a maximum likelihood estimator for the inversion distance between a
pair of genomes, using the group-theoretic approach to modelling inversions
introduced recently. This MLE functions as a corrected distance: in particular,
we show that because of the way sequences of inversions interact with each
other, it is quite possible for minimal distance and MLE distance to
differently order the distances of two genomes from a third. This has obvious
implications for the use of minimal distance in phylogeny reconstruction. The
work also tackles the above problem allowing free rotation of the genome.
Generally a frame of reference is locked, and all computation made accordingly.
This work incorporates the action of the dihedral group so that distance
estimates are free from any a priori frame of reference.Comment: 21 pages, 7 figures. To appear in the Journal of Theoretical Biolog
FeH Absorption in the Near-Infrared Spectra of Late M and L Dwarfs
We present medium-resolution z-, J-, and H-band spectra of four late-type
dwarfs with spectral types ranging from M8 to L7.5. In an attempt to determine
the origin of numerous weak absorption features throughout their near-infrared
spectra, and motivated by the recent tentative identification of the E 4\Pi- A
^4\Pi system of FeH near 1.6 microns in umbral and cool star spectra, we have
compared the dwarf spectra to a laboratory FeH emission spectrum. We have
identified nearly 100 FeH absorption features in the z-, J-, and H-band spectra
of the dwarfs. In particular, we have identified 34 features which dominate the
appearance of the H-band spectra of the dwarfs and which appear in the
laboratory FeH spectrum. Finally, all of the features are either weaker or
absent in the spectrum of the L7.5 dwarf which is consistent with the weakening
of the known FeH bandheads in the spectra of the latest L dwarfs.Comment: accepted by Ap
Ideas on DC-DC Converters for Delivery of Low Voltage and High Currents for the SLHC / ILC Detector Electronics in Magnetic field and Radiation environments
For more efficient power transport to the electronics embedded inside large colliding beam detectors, we explore the feasibility of supplying 48 Volts DC and using local DCDC conversion to 2 V (or lower, depending upon on the lithography of the embedded electronics) using switch mode regulators located very close to the front end electronics. These devices will be exposed to high radiation and high magnetic fields, 10 â 100 Mrads and 2 - 4 Tesla at the SLHC, and 20 Krads and 6 Tesla at the ILC
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