In vitro multiplication of Coffea arabica F1 hybrid (S.2800) and its performance in the field

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Somatic embryogenesis and plant-regeneration from inflorescence culture of java citronella (cymbopogon-winterianus)

Embryogenic callus was induced from immature inflorescence segments of Java citronella (Cymbopogon winterianus) and maintained for 2 years on Murashige and Skoog's medium supplemented with 2,4-D (l mg l−1). The callus cells retained the original chromosome number of 2n = 20. The somatic embryos germinated into plantlets on MS basal medium or medium with IAA, NAA, BAP or KN individually (l mg l−1). The regenerated plantlets developed a good root system on full strength solid MS inorganics medium with IAA (1 mg l−1). The regenerated plants were similar to the donor plant in morphology and had the same chromosome number, but showed some variation in the essential oil content