22 research outputs found
Nanostructured Systems Containing Rutin: In Vitro Antioxidant Activity and Photostability Studies
The improvement of the rutin photostability and its prolonged in vitro antioxidant activity were studied by means of its association with nanostructured aqueous dispersions. Rutin-loaded nanocapsules and rutin-loaded nanoemulsion showed mean particle size of 124.30 ± 2.06 and 124.17 ± 1.79, respectively, polydispersity index below 0.20, negative zeta potential, and encapsulation efficiency close to 100%. The in vitro antioxidant activity was evaluated by the formation of free radical ·OH after the exposure of hydrogen peroxide to a UV irradiation system. Rutin-loaded nanostructures showed lower rutin decay rates [(6.1 ± 0.6) 10â3 and (5.1 ± 0.4) 10â3 for nanocapsules and nanoemulsion, respectively] compared to the ethanolic solution [(35.0 ± 3.7) 10â3 minâ1] and exposed solution [(40.1 ± 1.7) 10â3 minâ1] as well as compared to exposed nanostructured dispersions [(19.5 ± 0.5) 10â3 and (26.6 ± 2.6) 10â3, for nanocapsules and nanoemulsion, respectively]. The presence of the polymeric layer in nanocapsules was fundamental to obtain a prolonged antioxidant activity, even if the mathematical modeling of the in vitro release profiles showed high adsorption of rutin to the particle/droplet surface for both formulations. Rutin-loaded nanostructures represent alternatives to the development of innovative nanomedicines
Preparation of Polymeric Micelles of Poly(Ethylene Oxide-b-Lactic Acid) and their Encapsulation With Lavender Oil
Nanoparticles comprised of the poly(ethylene oxide)-b-poly (lactic acid) diblock copolymer (PEO-b-PLA) with and without the incorporation of lavender oil were prepared by nanoprecipitation. Diblock copolymers based on a fixed PEO block (5KDa) and two different PLA segments (4.5 or 10KDa) were used. The morphology, encapsulation efficiency, essential oil-polymer interaction and the release kinetics of the active agent in the nanoparticles, were evaluated. The hydrodynamic radius of the nanoparticles determined by light scattering was affected by the size of the poly(lactic acid) (PLA) block. The lavender essential oil encapsulation efficiency (at a concentration of 0.4 ”L mL-1) determined by UV-VIS spectroscopy was in the range of 70-75%. The in vitro release suggests that the polymeric barrier is able to control the oil release