Ekspresija faktora rasta trombocita, njihovih receptora kao i subjedinice alfa integrina u postvakcinalnim sarkomima mačaka na mestu injekcije

Injection-site sarcomas are considered an important entity in veterinary oncology, especially in cats. The current study investigated the immunohistochemical expression of platelet-derived growth factor A (PDGFA), platelet-derived growth factor receptor-α (PDGFR-α), and integrin alpha subunit v in feline injection-site sarcomas (FISS). A total of 14 paraffin-embedded tissue samples previously diagnosed as soft tissue sarcomas were selected from the departmental archive and sectioned at 5µm using a microtome. Tissue sections were stained with Hematoxylin & Eosin, Alcian blue-Periodic Acid Schiff (AB-PAS), Masson’s Trichrome, and immunohistochemically for PDGFA, PDGR-α, and integrin alpha v. The majority of the tumors were fibrosarcomas (n=13/14), except one case of myxoid liposarcoma. The tumors occurred at sites commonly used for injections or vaccine administration. Histologically, tumors were characterized by peripheral infiltration of mononuclear cells mainly lymphocytes, variable sized central necrotic areas, and many multinucleated tumor giant cells. AB-PAS staining revealed the presence of myxoid or mucinous areas in (8/14) tumors while trichrome staining demonstrated a variable amount of collagenous stroma in 12/14 tumors. Vimentin immunoreactivity was observed in all the tumors while smooth muscle actin and muscle actin staining was noticed in four and two cases, respectively. PDGFA and PDGFR-α immunoexpression was observed in all 14 casesSmatra se da su sarkomi na mestu injekcije značajan patološki entitet u veterinarskoj onkologiji, naročito kod mačaka. Studija je ispitivala imunohistohemijsku ekspresiju faktora rasta A, poreklom iz trombocita (PDGFA), receptora za faktor rasta – alfa poreklom iz trombocita (PDGRF-α) i integrin alfa subjedinice v u slučajevima sarkoma na mestu inokulacije (FISS). Ukupno je ispitano 14 parafi nskih uzoraka, poreklom od prethodno dijagnostikovanih sarkoma mekih tkiva, iz podataka arhive departmana. Uzorci su standardno obrađeni i isečeni na isečke debljine od 5 µm pomoću mikrokriotoma. Uzorci tkiva su bojeni standardnom hematoksilin-eozin, Alcian plavo-Periodic Acid Schiff (AB-PAS) i Mason-trihrom tehnikama bojenja kao i imunohistohemijski u cilju detekcije PDGFA, PDGR-α kao i integrin-alfa v. Većina tumora su po svojim karakteristikama bili fi brosarkomi (n=13/14), osim u jednom slučaju miksoliposarkoma. Tumori su bili locirani na uobičajenim mestima inokulacije vakcina. Histološki u tumorima je uočena infi ltracija mononuklearnim ćelijama, većinom limfocitima, a u centralnim regionima nekrotična područja promenljive veličine, i prisustvom većieg broja multinuklearnih gigantocita. AB-PAS bojenjem, uočeno je prisustvo miksoidnih ili mucinoznih regiona kod 8 od 14 tumora dok je trihrom bojenje ukazalo na varijabilnu količinu kolagene strome kod 12 od ukupno 14 tumora. Kod svih tumora, uočena je imunoreaktivnost na vimentin, pri čemu je reaktivnost na glatkomišični aktin uočena kod četiri, odnosno kod dva slučaja. Imunoekspresija PDGFA kao i PDGFR-α zapašena je u svih 14 slučajeva pri čemu je pozitivan nalaz na integrin alfa bio u 13 od 14 slučajeva. Rezultati studije ukazuju da je morfološki fenotip FISS prevashodno u tipu fi brosarkoma. PDGFA, njegovi receptori i ekspresija integrin alfa v su povećani u slučaju ovih tumora mačaka, što ukazuje na njihovu ulogu u patogenezi FISS

Malathion ve Onosma nigricaule (Boraginaceae)'nin fare böbrek dokusunda katalaz (CAT) ve süperoksit dismutaz-2 (Mn-SOD) salınımı üzerine etkileri]

The purpose of this study was to determine the effects of the plant extract, which is obtained from Onosma nigricaule due to the oxidation parameters caused in mice by malathion that is used as an insecticide in agriculture, on catalase (CAT) and superoxide dismutase-2 (Mn-SOD) in kidney tissues by immunohistochemical technique. A total of 48 male mice (Mus musculus) were used in our study. Mice were divided 6 groups (control group, maize oil group, normal saline group, Onosma nigricaule group, malathion group, Onosma nigrcaule+malathion group). Hematoxylin-eosin and triple staining methods were used for histological and pathological examinations. The localization of CAT and Mn-SOD in the renal tissue was determined using the method of streptavidinbiotin- peroxidase. CAT immunoreactivity was determined with a weak intensity in epithelium of renal tubulus proximalis of mice in the malathion group, with a moderate intensity in Onosma nigricaule+ malathion group and with a higher intensity in tubulus proximalis of other groups. A cytoplasmic Mn-SOD immunoreactivity was determined with weak intensity in renal medulla of mice in malathion group, with moderate intensity in renal medulla of mice in Onosma nigricaule plant extract+ malathion group, maize oil group, and normal saline group and with highly intensity in control and Onosma nigricaule groups. It was concluded that Onosma nigricaule might play a protective role as an antioxidant against the oxidant features of malathion.Kafkas University, BAPKafkas University [2013-KSYO-78]This research was supported by a grant from the Kafkas University, BAP (Project No: 2013-KSYO-78

Alpha basic crystallin expression in canine mammary tumors

The aim of this study was to evaluate prognostic and/or diagnostic factors of canine mammary tumors by immunohistochemically analyzing the expression of alpha basic crystallin (αB-c). For this, formalin-fixed, paraffin-embedded blocks of 51 naturally-occurring canine mammary tumors (11 benign and 40 malignant) were used. Tissue from eight normal canine mammary glands were served as a control. Immunohistochemically, in the control mammary tissues, a few luminal epithelial cells were αB-c positive but myoepithelial cells were negative. In benign or simple type malignant tumors, αB-c expression was observed in luminal epithelial cells while the myoepithelial basal cells were negative. In benign or complex type malign tumors, positive staining was predominantly found in the cytoplasm of epithelial cells. Immunoreactivity of αB-c was also observed in neoplastic myoepithelial cells. Statistically, the number of cells immunolabeled with αB-c was found to be significantly different among tissues from normal canine mammary glands, benign lesions, and malignant tumors (p < 0.05). αB-c immunoreactivity was higher in malignant tumors than the control mammary tissues (p < 0.001). Data obtained in the current study revealed a strong association between high expression levels of αB-c and primary mammary gland tumors in canines

Expression of Platelet Derived Growth Factor a, Its Receptor, and Integrin Subunit Alpha V in Feline Injection-Site Sarcomas

Injection-site sarcomas are considered an important entity in veterinary oncology, especially in cats. The current study investigated the immunohistochemical expression of platelet-derived growth factor A (PDGFA), platelet-derived growth factor receptor-α (PDGFR-α), and integrin alpha subunit v in feline injection-site sarcomas (FISS). A total of 14 paraffin-embedded tissue samples previously diagnosed as soft tissue sarcomas were selected from the departmental archive and sectioned at 5µm using a microtome. Tissue sections were stained with Hematoxylin &amp; Eosin, Alcian blue-Periodic Acid Schiff (AB-PAS), Masson’s Trichrome, and immunohistochemically for PDGFA, PDGR-α, and integrin alpha v. The majority of the tumors were fibrosarcomas (n=13/14), except one case of myxoid liposarcoma. The tumors occurred at sites commonly used for injections or vaccine administration. Histologically, tumors were characterized by peripheral infiltration of mononuclear cells mainly lymphocytes, variable sized central necrotic areas, and many multinucleated tumor giant cells. AB-PAS staining revealed the presence of myxoid or mucinous areas in (8/14) tumors while trichrome staining demonstrated a variable amount of collagenous stroma in 12/14 tumors. Vimentin immunoreactivity was observed in all the tumors while smooth muscle actin and muscle actin staining was noticed in four and two cases, respectively. PDGFA and PDGFR-α immunoexpression was observed in all 14 casesSmatra se da su sarkomi na mestu injekcije značajan patološki entitet u veterinarskoj onkologiji, naročito kod mačaka. Studija je ispitivala imunohistohemijsku ekspresiju faktora rasta A, poreklom iz trombocita (PDGFA), receptora za faktor rasta – alfa poreklom iz trombocita (PDGRF-α) i integrin alfa subjedinice v u slučajevima sarkoma na mestu inokulacije (FISS). Ukupno je ispitano 14 parafi nskih uzoraka, poreklom od prethodno dijagnostikovanih sarkoma mekih tkiva, iz podataka arhive departmana. Uzorci su standardno obrađeni i isečeni na isečke debljine od 5 µm pomoću mikrokriotoma. Uzorci tkiva su bojeni standardnom hematoksilin-eozin, Alcian plavo-Periodic Acid Schiff (AB-PAS) i Mason-trihrom tehnikama bojenja kao i imunohistohemijski u cilju detekcije PDGFA, PDGR-α kao i integrin-alfa v. Većina tumora su po svojim karakteristikama bili fi brosarkomi (n=13/14), osim u jednom slučaju miksoliposarkoma. Tumori su bili locirani na uobičajenim mestima inokulacije vakcina. Histološki u tumorima je uočena infi ltracija mononuklearnim ćelijama, većinom limfocitima, a u centralnim regionima nekrotična područja promenljive veličine, i prisustvom većieg broja multinuklearnih gigantocita. AB-PAS bojenjem, uočeno je prisustvo miksoidnih ili mucinoznih regiona kod 8 od 14 tumora dok je trihrom bojenje ukazalo na varijabilnu količinu kolagene strome kod 12 od ukupno 14 tumora. Kod svih tumora, uočena je imunoreaktivnost na vimentin, pri čemu je reaktivnost na glatkomišični aktin uočena kod četiri, odnosno kod dva slučaja. Imunoekspresija PDGFA kao i PDGFR-α zapašena je u svih 14 slučajeva pri čemu je pozitivan nalaz na integrin alfa bio u 13 od 14 slučajeva. Rezultati studije ukazuju da je morfološki fenotip FISS prevashodno u tipu fi brosarkoma. PDGFA, njegovi receptori i ekspresija integrin alfa v su povećani u slučaju ovih tumora mačaka, što ukazuje na njihovu ulogu u patogenezi FISS

Investigation of Protective Effect of L-Carnitine on L-Asparaginase-Induced Acute Pancreatic Injury in Male Balb/c Mice

Introduction The present analysis deals with the biochemical and histopathological effects of L-carnitine in mice with L-asparaginase (ASNase)-induced experimental acute pancreatic injury (API)

Pyridine induction of cytochrome P450 1A1, iNOS and metallothionein in Syrian hamsters and protective effects of silymarin

An in vivo assessment for the protective effects of silymarin for pyridine toxicity was investigated through cytochrome P450 isoform CYP1A1 and inducible nitric oxide synthase (iNOS) activity prevention. Moreover, the effect of pyridine-induced oxidative stress on metallothionein I-II (MT), a scavenger of oxygen-derived free radicals, was investigated. Forty Syrian hamsters were allocated into 4 groups. Syrian hamsters were dosed with pyridine (400 mg/kg) intraperitoneally with and without silymarin (200 mg/kg daily by gavage) for 4 days. Pyridine induced diffuse degeneration and necrosis of the proximal and distal renal tubular cells; cloudy swelling, necrosis and hepatocellular atypia of the liver; and degenerative changes in the myocardium. The degree of pathological alterations was less severe with simultaneous silymarin application. CYP1A1, iNOS and MT expression levels were elevated in liver, kidney and heart in response to acute pyridine toxicity. Silymarin application abolished or significantly suppressed the induction of CYP1A1, iNOS and MT expressions in liver, kidney and heart of the pyridine-treated Syrian hamsters. Enhanced synthesis of MT by pyridine possibly implies a purposive cellular response to prevent damage caused by oxygen radicals. However, silymarin significantly reduced the oxidative-stress-inducing effect of pyridine as reflected by decreased synthesis of MT. These results suggest that through oxidant generation, pyridine may cause alteration of the metabolic ways, including nitric oxide-mediated CYP1A1 activity. (c) 2009 Elsevier GmbH. All rights reserved

Alpha-Galactosidase A Activity Levels in Turkish Male Hemodialysis Patients

WOS: 000311856500007PubMed ID: 23190516Fabry disease is an X-linked lysosomal storage disorder due to deficient activity of alpha-galactosidase A (alpha-Gal A) leading to renal insufficiency in males. The aim of present study was to investigate the level of alpha-Gal A activity and to determine the prevalence of Fabry disease in a Turkish male hemodialysis population. The activity of plasma alpha-Gal A was measured in a group of 808 male hemodialysis patients using fluorimetric methods. Patients with low alpha-Gal A activity were evaluated clinically and genetic testing was carried out. A correlation with creatinine, uric acid, urea, white blood cell (WBC), and high sensitivity (hs)CRP and alpha-Gal A activity was also investigated. Plasma a-Gal A activity among this male population undergoing hemodialysis was 7.88 +/- 5.18 mu M/hour/L (0.4055.72), significantly lower when compared to controls. No influence of creatinine, uric acid, WBC, or hsCRP on measured alpha-Gal A activity was reported. Two new Fabry disease patients were identified. Both were previously diagnosed with diabetes mellitus type 2. These findings provide, for the first time, data regarding the prevalence of alpha-Gal A deficiency (0.24%) in Turkish males receiving hemodialysis