Frequencia alélica e genotípica do gene dgat1 em uma população de bovinos de leite

DGAT1 gene is a candidate gene involved in fat content in milk and. This study analyzed DNA samples from 72 dairy cattle using blood samples. DNA was extracted use CTAB and proteinase K. The target region of the gene was amplified by PCR and the results were visualized with the use of polyacrylamide gel. The level of significance adopted was 5% for all analysis. Four alleles were checked their frequencies were: A= 4.0%, B= 50%, C= 40%, and D= 6.0%. It was verified the Hardy-Weinberg using the Chi square test concluding that the population was not in balance.O gene DGAT1 é um gene candidato envolvido com o conteúdo de gordura no leite. Neste estudo foram analisadas amostras de DNA de 72 bovinos leiteiros de uma população parcialmente fechada através amostras de sangue. O DNA foi extraído utilizando CTAB e proteinase K. A região alvo do gene foi amplificada por PCR e os resultados foram visualizados com a utilização de gel de poliacri- lamida. Na população em estudo foi verificada a presença de quatro alelos, cujas frequências foram: A= 4,0%, B= 50%, C= 40%, e D= 6,0%. Foi verificado o equilíbrio de Hardy-Weinberg através do teste de χ2, concluindo-se que a população não se encontrava em equilíbrio para o locos avaliado

Capim-piatã adubado com fontes de fósforo de diferente solubilidade em água

The development of piatã grass fertilized with phosphorus sources with different solubilities in water was evaluated. The experiment was developed in the unit of Aquidauana of UEMS, MS, from February to August, 2012. Four treatments were evaluated: control (without phosphorus fertilization), slow solubility, fast solubility (readily available), and mixed solubility sources of phosphorus (FH Pasture(r)). It was adopted a randomized blocks design with four blocks and four replicates in a total of 16 pastures (0.5 ha each one). Measurements were performed weekly to evaluate structural characteristics and morphogenic variables of the plants. Data were submitted to a variance analysis according to the model. When necessary, the means of treatments with different phosphorus fertilizer sources were compared with the control using the Dunnett test and with other treatments by using student's t test. The significance level of 5% was adopted in all analysis. No significant effect was observed (P<0.05) in the evaluated traits in the first year of the experiment

Detecção de locos de características quantitativas nos cromossomos 1, 2, 3, 12, 14, 15 e X de suínos: características de desempenho Detection of quantitative trait loci on chromosomes 1, 2, 3, 12, 14, 15, X in pigs: performance characteristics

Mapeou-se quantitative trait loci (QTL) associados a características de desempenho nos cromossomos 1, 2, 3, 12, 14, 15 e X de suínos pertencentes a uma população F2, formada a partir do cruzamento entre dois machos da raça naturalizada brasileira Piau e 18 fêmeas comerciais (Landrace x Large White x Pietrain). O mapa genético de ligação da população foi construído após a genotipagem dos animais para 35 marcadores microssatélites. As estimativas do conteúdo de informação polimórfica indicaram que os marcadores microssatélites foram adequados para as análises de QTL. Os dados foram analisados pelo mapeamento por intervalo usando-se o programa GridQTL. Encontraram-se seis QTL, sendo que o QTL genômico para idade ao abate atingiu a significância de 5% de probabilidade. As informações dos QTL detectados neste estudo são úteis para identificar genes que podem ser usados em conjunto com os métodos convencionais de seleção, aumentar a acurácia deles e prover uma compreensão dos fenótipos produtivos de suínos.The accomplishment of the present study had the objective of mapping Quantitative Trait Loci (QTL) related to performance traits in a F2 pig population developed by mating two Brazilian Piau breed sires with 18 dams from a commercial line (Landrace × Large White × Pietrain). The linkage map for this population was constructed after genotyping the animals for 35 microsatellite markers. Estimates of polymorphic information content indicated that the microsatellite markers were appropriate for QTL analyses. The genotypes were analyzed by interval mapping using the GridQTL program. A total of six QTL were found, of which the QTL for slaughter age (days) was significant at the 5% genome-wise level. The information of the significant QTL detected in this study is useful for future fine-mapping studies for the identification of genes. Such information can be used together with traditional methods in breeding programs or even for a better understanding of the phenotypes of swine production

Detection, epidemiology and characterization of VP6 and VP7 genes of group D rotavirus in broiler chickens

Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilMinistério da Agricultura. Laboratório Nacional Agropecuário. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Rotaviruses infect humans and animals and are classified into eight groups (A to H). Group D rotavirus (RVD) has been described in birds, although relatively few reports are available. The present study focused on RVD, including epidemiological and molecular aspects of samples collected from broiler chickens in the state of Pará, Brazil. A total of 85 faecal samples were collected between 2008 and 2011 from 37 chicken farms located in eight different municipalities. The viral double-stranded RNA was extracted from faecal suspensions and analysed using polyacrylamide gel electrophoresis (PAGE), followed by reverse transcriptase-polymerase chain reaction (RTPCR) and nucleotide sequencing of the VP6 and VP7 genes. Comparing the positive results, 16.5 per cent (14/85) were obtained by PAGE and 35.3 per cent (30/85) by RT-PCR. Samples from seven of eight municipalities were positive for RVD and infections were recorded in 17 (45.9 per cent) of 37 chicken farms. The RVD infection rate was significantly higher in the 16-day to 30-day age group (62.2 per cent; 23/37) compared with other ages. No consistent relationship was found between the infection rate and either the population density in poultry houses or the climatic conditions. The nucleotide sequences of the VP6 gene were 89.9 to 90.9 per cent similar to the prototype strain 05V0049 and were 88.3 to 100 per cent similar among themselves; VP7 gene nucleotide sequences were 84.3 to 85.4 per cent similar to the prototype strain 05V0049 and 93.8 to 100 per cent similar among themselves. Overall, this study provides new insights into the epidemiology and genome characterization of group D rotaviruse

Fixação biológica de nitrogênio e teores foliares de nutrientes na soja em função de doses de molibdênio e gesso agrícola Biological nitrogen fixation and leaf nutrient concentration on soybean as a function of molybdenum and gypsum levels

A competitividade econômica da soja brasileira no mercado mundial se deve, em grande parte, aos benefícios da fixação biológica do nitrogênio na cultura. O trabalho foi conduzido a campo, sob sistema plantio direto, em condição de sequeiro, no Município de Maracaju-MS, com o objetivo de avaliar a nodulação, o crescimento, nutrição mineral e produtividade de grãos da soja submetida a diferentes doses de gesso agrícola e molibdênio. Foi utilizado o delineamento experimental de blocos ao acaso, com cinco repetições e esquema de parcelas subdivididas, sendo as parcelas representadas por quatro doses de gesso agrícola (0, 1.000, 2.000 e 3.000 kg ha-1) e as subparcelas, por quatro doses de molibdênio (0, 20, 40 e 60 g ha-1). Não houve efeito da interação gesso x Mo sobre a produtividade da soja. O gesso agrícola não influencia no teor foliar de N e na produtividade. O Mo proporciona incrementos na produtividade e no teor de proteínas dos grãos.<br>The economic competitiveness of Brazilian soybeans on the world market occurs, in large part, due to the benefits of biological nitrogen fixation in this crop. The field experiment was carried out in Maracaju, Mato Grosso do Sul State, Brazil, under no-tillage system, in rainfed condition. The aim was to evaluate nodulation, growth, mineral nutrition and grain yield of soybeans under different doses of gypsum and molybdenum. The experimental design used was a randomized block with five replicates and arranged in a split-plot squeme, with the plot represented by four doses of gypsum (0, 1.000, 2.000 and 3.000 kg ha-1) and the subplots by four doses of molybdenum (0, 20, 40 and 60 g ha-1). There were no interaction effects of Mo x gypsum for grain yield. Gypsum has no influence in the N leaf content and grain yield. Mo increases grain yield and protein levels in the grain

Teor de proteína e de óleo nos grãos de soja em função do tratamento de sementes e aplicação de micronutrientes Protein and oil content in soybean grains resulting from treatment of the seeds and application of micronutrients

Conduziu-se este trabalho, com o objetivo de verificar o efeito do uso de inoculante, fungicida, e micronutrientes na produtividade e na composição química dos grãos de soja. Na safra de 2005/2006, foi realizado um estudo com diferentes tratamentos de sementes e aplicação foliar de micronutrientes. Avaliaram-se características agronômicas e a produtividade, assim como o teor de óleo, de proteína e de outros nutrientes das folhas e dos grãos de soja. Altura de planta foi influenciada significativamente pelos tratamentos aplicados, sendo que a menor média de altura de planta foi observada na testemunha, 89,75 cm. O diâmetro de caules, o número de vagens por planta e a massa de 100 sementes não foram influenciados pelos tratamentos. Nas condições que o experimento foi desenvolvido, em solos de boa fertilidade, os tratamentos adotados não influenciaram significativamente na produtividade e nos teores de proteína e de óleo dos grãos, assim como na composição química foliar e dos grãos de soja.<br>The objective of this work was to verify the effect of the use of inoculant, fungicide, and micronutrients on yield, and on the chemical composition of soybean grains. In the 2005/2006 crop, a study with different seed treatments and leaf applications of micronutrients was done. Agronomic characteristics and yield were evaluated, as well as, oil, protein, and nutritional contents of soybean leaves and grains. Plant height was influenced significantly by the applied treatments, with the smallest average of plant height being observed in control ( 89.75 cm). Stem diameter, number of beans per plant, and bio mass of 100 seeds were not influenced by treatments. Under the conditions in which the experiments were carried out, in soil with good fertility, the adopted treatments did not significantly influence yield, nor protein and oil content of grains, nor chemical composition of soybean leaves and grains