Development and evolution of dentition pattern and tooth order in the Skates and Rays (Batoidea; Chondrichthyes)

Shark and ray (elasmobranch) dentitions are well known for their multiple generations of teeth, with isolated teeth being common in the fossil record. However, how the diverse dentitions characteristic of elasmobranchs form is still poorly understood. Data on the development and maintenance of the dental patterning in this major vertebrate group will allow comparisons to other morphologically diverse taxa, including the bony fishes, in order to identify shared pattern characters for the vertebrate dentition as a whole. Data is especially lacking from the Batoidea (skates and rays), hence our objective is to compile data on embryonic and adult batoid tooth development contributing to ordering of the dentition, from cleared and stained specimens and micro-CT scans, with 3D rendered models. We selected species (adult and embryonic) spanning phylogenetically significant batoid clades, such that our observations may raise questions about relationships within the batoids, particularly with respect to current molecular-based analyses. We include developmental data from embryos of recent model organisms Leucoraja erinacea and Raja clavata to evaluate the earliest establishment of the dentition. Characters of the batoid dentition investigated include alternate addition of teeth as offset successional tooth rows (versus single separate files), presence of a symphyseal initiator region (symphyseal tooth present, or absent, but with two parasymphyseal teeth) and a restriction to tooth addition along each jaw reducing the number of tooth families, relative to addition of successor teeth within each family. Our ultimate aim is to understand the shared characters of the batoids, and whether or not these dental characters are shared more broadly within elasmobranchs, by comparing these to dentitions in shark outgroups. These developmental morphological analyses will provide a solid basis to better understand dental evolution in these important vertebrate groups as well as the general plesiomorphic vertebrate dental condition

Freezing behaviour facilitates bioelectric crypsis in cuttlefish faced with predation risk.

Cephalopods, and in particular the cuttlefish Sepia officinalis, are common models for studies of camouflage and predator avoidance behaviour. Preventing detection by predators is especially important to this group of animals, most of which are soft-bodied, lack physical defences, and are subject to both visually and non-visually mediated detection. Here, we report a novel cryptic mechanism in S. officinalis in which bioelectric cues are reduced via a behavioural freeze response to a predator stimulus. The reduction of bioelectric fields created by the freeze-simulating stimulus resulted in a possible decrease in shark predation risk by reducing detectability. The freeze response may also facilitate other non-visual cryptic mechanisms to lower predation risk from a wide range of predator types

Sensory systems in sawfishes. 1. The ampullae of Lorenzini

The distribution and density of the ampullary electroreceptors in the skin of elasmobranchs are influenced by the phylogeny and ecology of a species. Sensory maps were created for 4 species of pristid sawfish. Their ampullary pores were separated into pore fields based on their innervation and cluster formation. Ventrally, ampullary pores are located in 6 areas (5 in Pristis microdon), covering the rostrum and head to the gills. Dorsally, pores are located in 4 areas (3 in P. microdon), which cover the rostrum, head and may extend slightly onto the pectoral fins. In all species, the highest number of pores is found on the dorsal and ventral sides of the rostrum. The high densities of pores along the rostrum combined with the low densities around the mouth could indicate that sawfish use their rostrum to stun their prey before ingesting it, but this hypothesis remains to be tested. The directions of ampullary canals on the ventral side of the rostrum are species specific. P. microdon possesses the highest number of ampullary pores, which indicates that amongst the study species this species is an electroreception specialist. As such, juvenile P. microdon inhabit low-visibility freshwater habitats

Shallow water tidal flat use and associated specialized foraging behavior of the great hammerhead shark ( Sphyrna mokarran

Evidence suggests the great hammerhead shark, Sphyrna mokarran, is vulnerable to a variety of anthropogenic stressors, and is an understudied species of shark due to its cryptic nature and wide-ranging movements. While recognized as both a pelagic-coastal and a highly mobile predator, minimal anecdotal evidence exist describing shallow water habitat use by this species. This report describes six cases in which a great hammerhead shark utilizes an inshore shallow water flats environment (<1.5 m in depth), five of which involve prey capture. These observations permitted identification of two novel behaviors that may allow great hammerheads to inhabit these shallow habitats: a (1) prey-capture technique termed 'grasp-turning' that involves burst swimming at tight turning angles while grasping prey and (2) a post-predation recovery period whereby the shark maintains head-first orientation into the current that may facilitate respiration and prey consumption. These behavioral observations provide insights into the natural history of this species

Electroreception in elasmobranchs: sawfish as a case study

The ampullae of Lorenzini are the electroreceptors of elasmobranchs. Ampullary pores located in the elasmobranch skin are each connected to a gel-filled canal that ends in an ampullary bulb, in which the sensory epithelium is located. Each ampulla functions as an independent receptor that measures the potential difference between the ampullary pore opening and the body interior. In the elasmobranch head, the ampullary bulbs of different ampullae are aggregated in 3–6 bilaterally symmetric clusters, which can be surrounded by a connective tissue capsule. Each cluster is innervated by one branch of the anterior lateral line nerve (ALLN). Only the dorsal root of the ALLN carries electrosensory fibers, which terminate in the dorsal octavo-lateral nucleus (DON) of the medulla. Each ampullary cluster projects into a distinctive area in the central zone of the DON, where projection areas are somatotopically arranged. Sharks and rays can possess thousands of ampullae. Amongst other functions, the use of electroreception during prey localization is well documented. The distribution of ampullary pores in the skin of elasmobranchs is influenced by both the phylogeny and ecology of a species. Pores are grouped in distinct pore fields, which remain recognizable amongst related taxa. However, the density of pores within a pore field, which determines the electroreceptive resolution, is influenced by the ecology of a species. Here, I compare the pore counts per pore field between rhinobatids (shovelnose rays) and pristids (sawfish). In both groups, the number of ampullary pores on the ventral side of the rostrum is similar, even though the pristid rostrum can comprise about 20% of the total length. Ampullary pore numbers in pristids are increased on the upper side of the rostrum, which can be related to a feeding strategy that targets free-swimming prey in the water column. Shovelnose rays pin their prey onto the substrate with their disk, while repositioning their mouth for ingestion and thus possess large numbers of pores ventrally around the mouth and in the area between the gills