Effect of blood's velocity on blood resistivity

Blood resistivity is an important quantity whose value influences the results of various methods used in the study of heart and circulation. In this paper, the relationship between blood resistivity and velocity of blood flow was evaluated and analyzed based upon a probe using six-ring electrodes and a circulatory model. The experimental results indicated that the change in blood resistivity was only ±1.1% when the velocity of blood flow changed from 2.83 to 40 cm/s and it rose to 23% when the velocity was lower than 2.83 cm/s

High resolution single strand conformation polymorphism analysis using formamide and ethidium bromide staining

Single strand conformation polymorphism (SSCP) analysis using ethidium bromide can be improved by adding formamide as the denaturant. This gives higher resolution than previous SSCP methods; it had 100% sensitivity in the discrimination of 14 PCR samples from two different genes, even for a long fragment close to the upper limit of 250 base pairs. This modified procedure is a rapid, simple, safe, and yet highly sensitive method for detecting structural differences in DNA fragments.published_or_final_versio

Effects of l-dopa and tolcapone on COMT gene expression in human glial cells

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Analytical prediction of armature-reaction field in disc-type permanent magnet generators

H. C. Wong, Industrial CentreAuthor name used in this publication: S. L. HoAuthor name used in this publication: H. C. WongVersion of RecordPublishe

Polymerization-Induced Polymersome Fusion

The dynamic interactions of membranes, particularly their fusion and fission, are critical for the transmission of chemical information between cells. Fusion is primarily driven by membrane tension built up through membrane deformation. For artificial polymersomes, fusion is commonly induced via the external application of a force field. Herein, fusion-promoted development of anisotropic tubular polymersomes (tubesomes) was achieved in the absence of an external force by exploiting the unique features of aqueous ring-opening metathesis polymerization-induced self-assembly (ROMPISA). The out-of-equilibrium tubesome morphology was found to arise spontaneously during polymerization, and the composition of each tubesome sample (purity and length distribution) could be manipulated simply by targeting different core-block degrees of polymerization (DPs). The evolution of tubesomes was shown to occur via fusion of “monomeric” spherical polymersomes, evidenced most notably by a step-growth-like relationship between the fraction of tubular to spherical nano-objects and the average number of fused particles per tubesome (analogous to monomer conversion and DP, respectively). Fusion was also confirmed by Förster resonance energy transfer (FRET) studies to show membrane blending and confocal microscopy imaging to show mixing of the polymersome lumens. We term this unique phenomenon polymerization-induced polymersome fusion, which operates via the buildup of membrane tension exerted by the growing polymer chains. Given the growing body of evidence demonstrating the importance of nanoparticle shape on biological activity, our methodology provides a facile route to reproducibly obtain samples containing mixtures of spherical and tubular polymersomes, or pure samples of tubesomes, of programmed length. Moreover, the capability to mix the interior aqueous compartments of polymersomes during polymerization-induced fusion also presents opportunities for its application in catalysis, small molecule trafficking, and drug delivery

Different origins of visible luminescence in ZnO nanostructures fabricated by the chemical and evaporation methods

Zinc oxide nanostructures were fabricated using chemical and thermal evaporation methods. Scanning electron microscopy (SEM), x-ray diffraction, photoluminescence, and electron paramagnetic resonance (EPR) spectroscopy were used to study the properties of fabricated nanostructures. The nanostructures fabricated by evaporationg methods exhibited green PL from surface centers. The results show that the luminescence in the visible region has different peak positions in samples prepared by chemical and evaporation methods.published_or_final_versio

Low driving voltage band-filling-based III-V-on-silicon electroabsorption modulator

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Nucleoside Analogue Reverse Transcriptase Inhibitors Differentially Inhibit Human LINE-1 Retrotransposition

Intact LINE-1 elements are the only retrotransposons encoded by the human genome known to be capable of autonomous replication. Numerous cases of genetic disease have been traced to gene disruptions caused by LINE-1 retrotransposition events in germ-line cells. In addition, genomic instability resulting from LINE-1 retrotransposition in somatic cells has been proposed as a contributing factor to oncogenesis and to cancer progression. LINE-1 element activity may also play a role in normal physiology. LINE-1 retrotransposition reporter assay, we evaluated the abilities of several antiretroviral compounds to inhibit LINE-1 retrotransposition. The nucleoside analogue reverse transcriptase inhibitors (nRTIs): stavudine, zidovudine, tenofovir disoproxil fumarate, and lamivudine all inhibited LINE-1 retrotransposition with varying degrees of potencies, while the non-nucleoside HIV-1 reverse transcriptase inhibitor nevirapine showed no effect.Our data demonstrates the ability for nRTIs to suppress LINE-1 retrotransposition. This is immediately applicable to studies aimed at examining potential roles for LINE-1 retrotransposition in physiological processes. In addition, our data raises novel safety considerations for nRTIs based on their potential to disrupt physiological processes involving LINE-1 retrotransposition