189 research outputs found

    229: Umbilical cord blood transplantation for adult patients with chronic myeloid leukemia

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    Florescimento do quiabeiro cv "Amarelinho" em resposta a diferentes densidades de plantas.

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    O quiabeiro, cv. Amarelinho, apresenta uma baixa taxa de florescimento possivelmente, em decorrência da combinação de sua arquitetura com o espaçamento inadequado. Com base nesta particularidade, o presente trabalho teve por objetivo avaliar o florescimento da planta em resposta a diferentes densidades de plantio.Edição dos Resumos do 45º Congresso Brasileiro de Olericultura; 15º Congresso Brasileiro de Floricultura e Plantas Ornamentais; 2º Congresso Brasileiro de Cultura de Tecidos de Plantas, Fortaleza, 07 a 12 Agosto de 2005

    Avaliação dos aspectos fenológicos na emissão de ramos produtivos do quiabeiro cv. 'Amarelinho' em resposta a variação da densidade de plantio.

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    A planta do quiabeiro embora apresente algumas diversidades no seu aspecto morfológico, podem sofrer variações de comportamento em função do habitat a que estão submetidas e do sistema de plantio. Neste contexto, o trabalho teve por objetivo avaliar os efeitos do espaçamento na emissão de ramos produtivos e improdutivos do quiabeiro, cv. Amarelinho.Edição dos Resumos do 45º Congresso Brasileiro de Olericultura; 15º Congresso Brasileiro de Floricultura e Plantas Ornamentais; 2º Congresso Brasileiro de Cultura de Tecidos de Plantas, Fortaleza, 07 a 12 Agosto de 2005

    Stem cell transplantation in 40 pts with Fanconi anemia (FA): Excellent survival and low toxicity for pts with a related HLA identical donor

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    Univ Fed Parana, BR-80060000 Curitiba, Parana, BrazilEPM, Inst Oncol Pediat, São Paulo, BrazilEPM, Inst Oncol Pediat, São Paulo, BrazilWeb of Scienc

    Produção de sementes de quiabeiro cv. Amarelinho: efeito da densidade de plantio e localização na planta.

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    O presente trabalho teve por objetivou estudar a produção de sementes quiabeiro em função de diferentes populações de planta. Foi utilizado a cv. Amarelinho no ensaio conduzido em condições de campo na Fazenda Experimental São Manuel/UNESP/SP sendo submetida a nove combinações de espaçamentos entrelinhas (1,00m; 1,25m e 1,50m) e entre plantas (0,30m; 0,45m e 0,60m). O delineamento utilizado foi em blocos casualizados com seis repetições no esquema fatorial 3x3, adotando a comparação de médias pelo teste Tukey ao nível de 5% de probabilidade. Os parâmetros avaliados foram a produção de sementes por planta, por haste principal e ramos. Para os espaçamentos menores, maior foi a produção de sementes devido ao maior número de plantas por área. Porém, os maiores espaçamentos justificaram uma maior produção por planta devido ao maior número de hastes produtivas.Edição dos Anais do 47° Congresso Brasileiro de Olericultura; 4º Simpósio Brasileiro sobre Cucurbitáceas, Porto Seguro, ago. 2007. 1 CD-ROM

    Dynamic expression of Ralstonia solanacearum virulence factors and metabolism-controlling genes during plant infection

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    Altres ajuts: CERCA Programme/Generalitat de Catalunya. P. Sebastià received the support of a fellowship (code is LCF/BQ/IN17/11620004) from la Caixa Foundation (identifier [ID] 100010434)Background: Ralstonia solanacearum is the causal agent of bacterial wilt, a devastating plant disease responsible for serious economic losses especially on potato, tomato, and other solanaceous plant species in temperate countries. In R. solanacearum, gene expression analysis has been key to unravel many virulence determinants as well as their regulatory networks. However, most of these assays have been performed using either bacteria grown in minimal medium or in planta, after symptom onset, which occurs at late stages of colonization. Thus, little is known about the genetic program that coordinates virulence gene expression and metabolic adaptation along the different stages of plant infection by R. solanacearum. Results: We performed an RNA-sequencing analysis of the transcriptome of bacteria recovered from potato apoplast and from the xylem of asymptomatic or wilted potato plants, which correspond to three different conditions (Apoplast, Early and Late xylem). Our results show dynamic expression of metabolism-controlling genes and virulence factors during parasitic growth inside the plant. Flagellar motility genes were especially up-regulated in the apoplast and twitching motility genes showed a more sustained expression in planta regardless of the condition. Xylem-induced genes included virulence genes, such as the type III secretion system (T3SS) and most of its related effectors and nitrogen utilisation genes. The upstream regulators of the T3SS were exclusively up-regulated in the apoplast, preceding the induction of their downstream targets. Finally, a large subset of genes involved in central metabolism was exclusively down-regulated in the xylem at late infection stages. Conclusions: This is the first report describing R. solanacearum dynamic transcriptional changes within the plant during infection. Our data define four main genetic programmes that define gene pathogen physiology during plant colonisation. The described expression of virulence genes, which might reflect bacterial states in different infection stages, provides key information on the R. solanacearum potato infection process

    Next-Generation Phage Display: Integrating and Comparing Available Molecular Tools to Enable Cost-Effective High-Throughput Analysis

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    Background: Combinatorial phage display has been used in the last 20 years in the identification of protein-ligands and protein-protein interactions, uncovering relevant molecular recognition events. Rate-limiting steps of combinatorial phage display library selection are (i) the counting of transducing units and (ii) the sequencing of the encoded displayed ligands. Here, we adapted emerging genomic technologies to minimize such challenges. Methodology/Principal Findings: We gained efficiency by applying in tandem real-time PCR for rapid quantification to enable bacteria-free phage display library screening, and added phage DNA next-generation sequencing for large-scale ligand analysis, reporting a fully integrated set of high-throughput quantitative and analytical tools. The approach is far less labor-intensive and allows rigorous quantification; for medical applications, including selections in patients, it also represents an advance for quantitative distribution analysis and ligand identification of hundreds of thousands of targeted particles from patient-derived biopsy or autopsy in a longer timeframe post library administration. Additional advantages over current methods include increased sensitivity, less variability, enhanced linearity, scalability, and accuracy at much lower cost. Sequences obtained by qPhage plus pyrosequencing were similar to a dataset produced from conventional Sanger-sequenced transducing-units (TU), with no biases due to GC content, codon usage, and amino acid or peptide frequency. These tools allow phage display selection and ligand analysis at.1,000-fold faster rate, and reduce costs,250fol
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