Biosynthesis of Sepedonin by Cell Free Extract of Sepedonium Chrysospermum

A supernatant obtained by centrifugation at 20.000 g from homogenate of mycelium of Sepedonium chrysospermum was proved to be capable of incorporating acetyl-CoA, malonyl-CoA and methionine into sepedonin. This biosynthesis required O_2 in the atmosphere and a cofactor such as NADH, NADPH or NADP^+, among which NADP^+ was proved to be most effective. Incorporation of methionine into sepedonin was enhanced by addition of vitamin B_ and suppressed by ethionine or iodoacetamide

Solid Phase Synthesis of the Active Center Octapeptide of Candida krusei Cytochrome C Apoprotein

The active center octapeptide of Candida krusei cytochrome C apoprotein, H_2N-Cys-Ala-Glu-Cys-His-Thr-Ile-Glu-OH, was synthesized by the Merrifield method using an automatic peptide synthesizer

Solid Phase Synthesis of a Nonapeptide of Melittin

The venom of the honey bee, Apris mellifica, is a strongly hemolytic polypeptide termed Melittin. It contains 26 amino acids, and the complete amino acid sequence of Melittin has recently been published^. This paper reports the synthesis of a nonapeptide. Gly-Ile-Gly-Ala-Val-Leu-Lys-Val-Leu, of Melittin. An instrument, which can perform both automatically and manually all of the operation in the stepwise synthesis of peptide by the solid phase method, was constructed^. The peptide was synthesized using manually the instrument. The peptide was purified by recrystallization and characterized by paper electrophoresis and amino acid analysis, and obtained in over all yield of 90%

On the Structures of the Methylation Products of Sepedonin Derivatives with Diazomethane

Methylation of anhydrosepedonin and deoxysepedonin with diazomethane afforded three different kinds of dimethyl ethers. The structures of these ethers were examined by their reaction with liquid ammonia, and long-range coupling between methoxyl protons and the adjacent ring proton by nmr spectra from which the structures of 3, 4-dimethoxy, 2, 6-dimethoxy, and 2, 4-dimethoxy derivatives were clarified. The mass spectra of these dimethyl ethers showed the difference in the position of these methoxyls

Pre-DECIGO can get the smoking gun to decide the astrophysical or cosmological origin of GW150914-like binary black holes

Pre-DECIGO consists of three spacecraft arranged in an equilateral triangle with 100km arm lengths orbiting 2000km above the surface of the earth. It is hoped that the launch date will be in the late 2020s. Pre-DECIGO has one clear target: binary black holes (BBHs) like GW150914 and GW151226. Pre-DECIGO can detect $\sim 30M_\odot-30M_\odot$ BBH mergers up to redshift $z\sim 30$. The cumulative event rate is $\sim 1.8\times 10^{5}\,{\rm events~yr^{-1}}$ in the Pop III origin model of BBHs like GW150914, and it saturates at $z\sim 10$, while in the primordial BBH (PBBH) model, the cumulative event rate is $\sim 3\times 10^{4}\,{\rm events~ yr^{-1}}$ at $z=30$ even if only $0.1\%$ of the dark matter consists of PBHs, and it is still increasing at $z=30$. In the Pop I/II model of BBHs, the cumulative event rate is $(3-10)\times10^{5}\,{\rm events~ yr^{-1}}$ and it saturates at $z \sim 6$. We present the requirements on orbit accuracy, drag free techniques, laser power, frequency stability, and interferometer test mass. For BBHs like GW150914 at 1Gpc, SNR$\sim 90$ is achieved with the definition of Pre-DECIGO in the $0.01-100$Hz band. Pre-DECIGO can measure the mass spectrum and the $z$-dependence of the merger rate to distinguish various models of BBHs like GW150914. Pre-DECIGO can also predict the direction of BBHs at $z=0.1$ with an accuracy of $\sim 0.3\,{\rm deg}^2$ and a merging time accuracy of $\sim 1$s at about a day before the merger so that ground-based GW detectors further developed at that time as well as electromagnetic follow-up observations can prepare for the detection of merger in advance. For intermediate mass BBHs at a large redshift $z > 10$, the QNM frequency after the merger can be within the Pre-DECIGO band so that the ringing tail can also be detectable to confirm the Einstein theory of general relativity with SNR$\sim 35$. [abridged]Comment: 17 pages, 10 figures, added some references, modifications to match the published version in PTE

Study on Anesthetic-protein Interactions of Model Protein of Regulator Enzyme of Channel Receptor

科学研究費補助金研究成果報告書研究種目: 基盤研究(C)研究期間: 2003～2004課題番号: 15591625研究代表者: 瀬戸 倫義（滋賀医科大学・医学部・講師）研究分担者: 犬伏 俊郎（滋賀医科大学・分子神経科学センター・教授）研究分担者: 野坂 修一（滋賀医科大学・医学部・教授

Electrophysiological study of the mechanism of unconsciousness of anesthesia

科学研究費補助金研究成果報告書研究種目: 基盤研究(B)研究期間: 2002～2004課題番号: 14370485研究代表者: 竹之下 眞（滋賀医科大学・医学部・助教授）研究分担者: 瀬戸 倫義（滋賀医科大学・医学部・講師）研究分担者: 佐井 義和（滋賀医科大学・医学部・助教授）研究分担者: 野坂 修一（滋賀医科大学・医学部・教授）研究分担者: 牧浦 弥惠子（滋賀医科大学・医学部・助手

Coincidence analysis to search for inspiraling compact binaries using TAMA300 and LISM data

Japanese laser interferometric gravitational wave detectors, TAMA300 and LISM, performed a coincident observation during 2001. We perform a coincidence analysis to search for inspiraling compact binaries. The length of data used for the coincidence analysis is 275 hours when both TAMA300 and LISM detectors are operated simultaneously. TAMA300 and LISM data are analyzed by matched filtering, and candidates for gravitational wave events are obtained. If there is a true gravitational wave signal, it should appear in both data of detectors with consistent waveforms characterized by masses of stars, amplitude of the signal, the coalescence time and so on. We introduce a set of coincidence conditions of the parameters, and search for coincident events. This procedure reduces the number of fake events considerably, by a factor $\sim 10^{-4}$ compared with the number of fake events in single detector analysis. We find that the number of events after imposing the coincidence conditions is consistent with the number of accidental coincidences produced purely by noise. We thus find no evidence of gravitational wave signals. We obtain an upper limit of 0.046 /hours (CL $= 90$) to the Galactic event rate within 1kpc from the Earth. The method used in this paper can be applied straightforwardly to the case of coincidence observations with more than two detectors with arbitrary arm directions.Comment: 28 pages, 17 figures, Replaced with the version to be published in Physical Review