Small-angle X-ray Scattering And Structural Modeling Of Full-length: Cellobiohydrolase I From Trichoderma Harzianum

Cellobiohydrolases hydrolyze cellulose releasing cellobiose units. They are very important for a number of biotechnological applications, such as, for example, production of cellulosic ethanol and cotton fiber processing. The Trichoderma cellobiohydrolase I (CBH1 or Cel7A) is an industrially important exocellulase. It exhibits a typical two domain architecture, with a small C-terminal cellulose-binding domain and a large N-terminal catalytic core domain, connected by an O-glycosylated linker peptide. The mechanism by which the linker mediates the concerted action of the two domains remains a conundrum. Here, we probe the protein shape and domain organization of the CBH1 of Trichoderma harzianum (ThCel7A) by small angle X-ray scattering (SAXS) and structural modeling. Our SAXS data shows that ThCel7A linker is partially-extended in solution. 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"Setentão" uma nova cultivar de feijão-de-corda para o estado do Ceará

Do cruzamento entre a cultivar "sempre verde" de semente de tegumento creme e a cultivar "TVu 59", de sementes de cor marrom, selecionou-se a cultivar "Setentão". O método utilizado foi o genealógico com teste de capacidade produtiva nas gerações F3 e F4, sendo as melhores linhas submetidas a seleção massal em blocos isolados e separados por fileiras de milho na geração F5, na geração F5 foi realizada seleção individual dentro de cada linha, iniciando-se na geração F7 os ensaios de produção com 14 linhas. A seleção 010.061 (05) (01) (03) além da excelente qualidade da semente, apresenta boa capacidade produtiva, resistência ao CpSMV e CMV, respectivamente. Esta seleção agora cognominada de "Setentão" apresenta hábito de crescimento indeterminado, porte semi-ramador e boa produtividade.<br>A cultivar of cowpea, Vigna unguiculata, named "Setentão" was obtained from a cross between "Sempre Verde" with seeds with cream integument and "TVu 59" with seeds with brown integument. The genealogical method with productive capacity test was used in the selection for F3 and F4 generations, with the best lines submitted to a bulk selection in isolated blocks (with lines separated by maige) in the F5 generation. Individual selection was carried out within each line and the assay productions with 14 lines were initiated at the F7. The selected line 010.061 (05) (01) (03) presented excellent seed quality together with good productive capacity and resistance to cowpea severe mosaic virus and cucumber mosaic virus isolated in Ceará from cowpea. The selected line named "Setentão" has indeterminate growth with semi-climbing habit and good productivity