Effect of Water Potential on Germination of Verticillum dahliae microsclerotia

The effects of osmotic and matric potentials on the microsclerotial germination of Verticillium dahliae was examined at room temperature in 1% water agar amended with sodium chloride and polyethylene glycole. Treatments consisted of 6 levels of osmotic and matric potentials (0, -0.3, -0.6, -0.9, -1.2, and -1.5 MPa) laid out as factorial arrangement in a completely randomized design. Decreasing matric potential reduced germination, whereas the osmotic potential increased germination up to -0.6 MPa but any further increase caused it to decline. It was concluded that the matric potential is a more limiting factor than the osmotic potential for the germination of V. dahliae microsclerotia

Estimativa da área foliar do pepino em ambiente protegido por medidas lineares sob salinidade e enxertia

The measurement of leaf area by linear parameters is a useful tool when plants cannot be destroyed for direct measurement. The objectives of this study were to establish equations to estimate the leaf area of greenhouse-cucumber and to evaluate the effects of salinity and grafting on this estimative. Non-grafted cucumber seedlings, cv. 'Hokushin', were transplanted in a greenhouse and were irrigated with water of different salinities (1.0, 3.2 and 5.0 dS m-1). In the second growing period, the same cultivar was grafted on Cucurbita spp. and the plants were irrigated with water of 1.4, 3.0 and 5.3 dS m-1. Leaves of different sizes were collected from both experiments and leaf area was determined by an integrating area meter. Leaf length (L) and width (W) were also recorded. An equation for estimating the leaf area from L and W was developed for a given salinity level or grafting condition and estimated well the area of leaves collected in the other treatments. The leaf area (LA) of cucumber 'Hokushin' could be estimated using the equation LA = 0.88LW - 4.27, for any grafting and salinity conditions.A determinação da área foliar por medidas lineares é uma ferramenta útil quando as plantas não podem ser destruídas para que a medição direta seja realizada. Os objetivos desse trabalho foram definir equações para a estimativa da área foliar do pepino em ambiente protegido e avaliar os efeitos da salinidade e da a enxertia nessa estimativa. Mudas de pepino, cv. 'Hokushin', não enxertadas, foram transplantadas em um ambiente protegido e irrigadas com água de diferentes salinidades (1,0, 3,2 e 5,0 dS m-1). No segundo período de cultivo, a mesma cultivar foi enxertada sobre Cucurbita spp., sendo as plantas irrigadas com água de 1,4, 3,0 e 5,3 dS m-1. Foram coletadas folhas de diferentes tamanhos dos dois cultivos e dos três tratamentos e a área foliar foi determinada por um medidor de área foliar. O comprimento (C) e a largura (L) da folha também foram registrados. Desenvolveram-se equações pelas quais a área foliar pôde ser estimada a partir de medidas de C e L. A equação desenvolvida para um dado nível de salinidade ou condição de enxertia estimou bem a área das folhas coletadas nos demais tratamentos. A área foliar (AF) do pepino 'Hokushin' pode ser estimada pela função AF = 0,88CL - 4,27, para qualquer condição de enxertia e salinidade

Yield, water and nitrogen-use response of rice to zeolite and nitrogen fertilization in a semi-arid environment

Water scarcity and soil nitrogen (N) loss are important limitations for agricultural production in semi-arid region especially for rice production. Zeolite (Z) as a soil conditioner can be used to retrain water and nitrogen in near-surface soil layer in lowland rice production system. The objectives of this study were to investigate the effects of different application rates of natural zeolite (clinoptilolite) and nitrogen on rice yield, yield components, soil nitrogen, water use, water productivity in a silty clay soil in 2004 and 2005. Zeolite was only applied in the first year. In order to study the long-term and continuous effect of zeolite on the objectives of the study, no zeolite was applied in the second year and the study was conducted on the same land as the first year. Zeolite and N were applied at rates of 0, 2, 4, and 8 t ha-1 and 0, 20, 40, and 80 kg ha-1, respectively in 2004. In 2005, each plot received the same amount of N as received in 2004. It is concluded that by decreasing N application rates, higher Z application rate is needed to improve grain yield. Highest grain yield was obtained at N application rate of 80 kg ha-1 and Z application rate of 4 t ha-1. Higher grain yield was mostly attributed to lower unfilled grain percentage and higher 1000-grain weight that were a result of higher N application rate and N retention in soil due to Z application. Nitrogen and Z applications resulted in higher grain protein contents and nitrogen recovery efficiency (NRE). Based on these results and due to higher N retention in soil under Z application, improved grain yield quality, nitrogen-use efficiency (NUE), and nitrogen recovery efficiency (NRE) could be obtained at Z application rate of 8 t ha-1 and N application rate of 80 kg ha-1 or more. However, this was not satisfied for NUE. Moreover, it is found that at higher N application rates lower Z application rates are needed to effectively retain soil residual mineral nitrogen. Furthermore, at N application rates of 80 kg ha-1 or more, Z application increased soil water retention and resulted in lower seasonal water use and higher water productivity. In general, it was concluded that the effect of Z application in retaining soil N was also effective in the second year.Clinoptilolite Nitrogen recovery Nitrogen-use efficiency Rice Zeolite Water productivity

Deficit irrigation and nitrogen effects on nitrogen-use efficiency and grain protein of rice

To meet future food demand, world rice production must increase in the next few decades, which is possible only by effective use of soil and water resources and inputs. This research was conducted to investigate the effects of nitrogen, N, application and deficit irrigation treatments including a sprinkler, intermittent flooding and continuous flood irrigation, and their interaction with the N-use efficiency and grain protein of a local lowland rice cultivar. The results indicated that low (sprinkler irrigation) and high (continuous flood irrigation) applied water affected the plant and soil factors in N uptake and decreased N-use efficiency for rice. Therefore, optimum applied water was obtained in intermittent flooding (2-day interval). Reduction in nitrogen uptake at high applied water can be due to the effect of nitrate leaching in the root zone and the reduction in N uptake at low applied water can be due to the inability of the roots to absorb N and translocate it to the plant top. With respect to the relationship between N uptake and grain protein and leaf chlorophyll, these parameters can also be affected by applied water and N application. Appropriate linear models were proposed to show these relationships. At different times of soil nitrogen measurements and N application rates, maximum nitrogen leaching (about 50%) occurred in continuous flooding irrigation