12 research outputs found
Cytotoxicity and DNA damage in the neutrophils of patients with sickle cell anaemia treated with hydroxyurea
Hydroxyurea (HU) is the most important advance in the treatment of sickle cell anaemia (SCA) for preventing complications and improving quality of life for patients. However, some aspects of treatment with HU remain unclear, including their effect on and potential toxicity to other blood cells such as neutrophils. This study used the measurement of Lactate Dehydrogenase (LDH) and Methyl ThiazolTetrazolium (MTT) and the comet assay to investigate the cytotoxicity and damage index (DI) of the DNA in the neutrophils of patients with SCA using HU.In the LDH and MTT assays, a cytoprotective effect was observed in the group of patients treated, as well as an absence of toxicity. When compared to patients without the treatment, the SS group (n=20, 13 women and 07 men, aged 18-69 years), and the group of healthy individuals (AA) used as a control group (n=52, 28 women and 24 men, aged 19-60 years), The SSHU group (n=21, 11 women and 10 men, aged 19-63 years) showed a significant reduction (p20 months), demonstrating that despite the cytoprotective effects in terms of cell viability, the use of HU can induce DNA damage in neutrophils
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Model for the control of potassium transport in PHA-stimulated human blood lymphocytes
Results are reported for studies on the following: lymphocyte plasma-membrane ATP ase; substrate specificity of lymphocyte membrane phosphatase activity; effect of PHA on lymphocyte membrane ATP ase; correlation of K/sup +/ with lymphocyte K/sup +/ concentration; and K/sup +/ efflux, influx, and cell concentration in PHA-treated lymphocytes. (HLW