14,109 research outputs found
The avian tectobulbar tract: development, explant culture, and effects of antibodies on the pattern of neurite outgrowth
The tectobulbar tract is the first long-distance projecting fiber pathway to appear during the development of the avian optic tectum (dorsal half of the mesencephalon). Immunologically stained wholemounts of the E3 mesencephalon reveal that the early tectobulbar axons course in a dorsal-to-ventral direction and abruptly turn in a caudal direction shortly before reaching the ventral midline. During subsequent development, more tectobulbar axons are generated that form a parallel array of thick fascicles coursing ventrally within the mesencephalon. At this later stage of development, the tectobulbar tract bifurcates into an ipsilateral and contralateral pathway, both growing in a caudal direction near the mesencephalic ventral midline. Bifurcation and change in direction of growth is accompanied by a complete loss of the fasciculated growth pattern. Each tectobulbar axon is thus divided into a proximal fasciculated and a distal unfasciculated segment. Tectobulbar fascicles occupy the most superficial surface layer of the mesencephalon at early stages and are displaced deeper into the tissue beginning at embryonic day 5. In both of these locations, tectobulbar axons express molecules involved in axon-axon and axon-substrate interactions like the G4 antigen, neural cell adhesion molecule (N-CAM), neurofascin, and T61 antigen as revealed by immunohistochemistry and immunoblotting. Stripes of the mesencephalon explanted onto a basal lamina substratum show vigorous outgrowth of neurites. These processes grow in fascicles at a growth rate of 40 microns/h. Staining of the neurites with specific antibodies, as well as the position of the retrogradely labeled cell bodies, is in agreement with these processes being tectobulbar axons. This in vitro explant system was used to investigate the expression and possible functional involvement of N-CAM, neurofascin, G4 protein, and T61 antigen in the growth of these axons. The presence of antigen- binding fragments of polyclonal anti-G4 antibodies completely blocks fasciculation of the neurites but has no influence on their rate of elongation. Antibodies against N-CAM and neurofascin have no detectable effects. The number and length of the in vitro growing axons are reduced by the monoclonal T61 antibody. This effect is reversible. The elucidation of the exact course in vivo and the accessibility to the axons growing in vitro make the tectobulbar tract an excellent model system for the investigation of the role of these and other proteins in axonal growth and guidance during the development of the CNS
Stochastic dynamics of adhesion clusters under shared constant force and with rebinding
Single receptor-ligand bonds have finite lifetimes, so that biological
systems can dynamically react to changes in their environment. In cell
adhesion, adhesion bonds usually act cooperatively in adhesion clusters.
Outside the cellular context, adhesion clusters can be probed quantitatively by
attaching receptors and ligands to opposing surfaces. Here we present a
detailed theoretical analysis of the stochastic dynamics of a cluster of
parallel bonds under shared constant loading and with rebinding. Analytical
solutions for the appropriate one-step master equation are presented for
special cases, while the general case is treated with exact stochastic
simulations. If the completely dissociated state is modeled as an absorbing
boundary, mean cluster lifetime is finite and can be calculated exactly. We
also present a detailed analysis of fluctuation effects and discuss various
approximations to the full stochastic description.Comment: Revtex, 29 pages, 23 postscript figures included (some with reduced
image quality
Bending Frustration of Lipid-Water Mesophases Based on Cubic Minimal Surfaces
Inverse bicontinuous cubic phases are ubiquitous in lipid-water mixtures and
consist of a lipid bilayer forming a cubic minimal surface, thereby dividing
space into two cubic networks of water channels. For small hydrocarbon chain
lengths, the monolayers can be modeled as parallel surfaces to a minimal
midsurface. The bending energy of the cubic phases is determined by the
distribution of Gaussian curvature over the minimal midsurfaces which we
calculate for seven different structures (G, D, P, I-WP, C(P), S and F-RD). We
show that the free-energy densities of the structures G, D and P are
considerably lower than those of the other investigated structures due to their
narrow distribution of Gaussian curvature. The Bonnet transformation between G,
D, and P implies that these phases coexist along a triple line, which also
includes an excess water phase. Our model includes thermal membrane
undulations. Our qualitative predictions remain unchanged when higher order
terms in the curvature energy are included. Calculated phase diagrams agree
well with the experimental results for 2:1 lauric acid/dilauroyl
phosphatidylcholine and water.Comment: Revtex, 23 pages with 9 postscript figures included, to appear in
Langmui
Phase behavior and material properties of hollow nanoparticles
Effective pair potentials for hollow nanoparticles like the ones made from
carbon (fullerenes) or metal dichalcogenides (inorganic fullerenes) consist of
a hard core repulsion and a deep, but short-ranged, van der Waals attraction.
We investigate them for single- and multi-walled nanoparticles and show that in
both cases, in the limit of large radii the interaction range scales inversely
with the radius, , while the well depth scales linearly with . We predict
the values of the radius and the wall thickness at which the gas-liquid
coexistence disappears from the phase diagram. We also discuss unusual material
properties of the solid, which include a large heat of sublimation and a small
surface energy.Comment: Revtex, 13 pages with 8 Postscript files included, submitted to Phys.
Rev.
Collective effects in cellular structure formation mediated by compliant environments: a Monte Carlo study
Compliant environments can mediate interactions between mechanically active
cells like fibroblasts. Starting with a phenomenological model for the
behaviour of single cells, we use extensive Monte Carlo simulations to predict
non-trivial structure formation for cell communities on soft elastic substrates
as a function of elastic moduli, cell density, noise and cell position
geometry. In general, we find a disordered structure as well as ordered
string-like and ring-like structures. The transition between ordered and
disordered structures is controlled both by cell density and noise level, while
the transition between string- and ring-like ordered structures is controlled
by the Poisson ratio. Similar effects are observed in three dimensions. Our
results suggest that in regard to elastic effects, healthy connective tissue
usually is in a macroscopically disordered state, but can be switched to a
macroscopically ordered state by appropriate parameter variations, in a way
that is reminiscent of wound contraction or diseased states like contracture.Comment: 45 pages, 7 postscript figures included, revised version accepted for
publication in Acta Biomateriali
Elastic Interactions of Cells
Biological cells in soft materials can be modeled as anisotropic force
contraction dipoles. The corresponding elastic interaction potentials are
long-ranged ( with distance ) and depend sensitively on elastic
constants, geometry and cellular orientations. On elastic substrates, the
elastic interaction is similar to that of electric quadrupoles in two
dimensions and for dense systems leads to aggregation with herringbone order on
a cellular scale. Free and clamped surfaces of samples of finite size introduce
attractive and repulsive corrections, respectively, which vary on the
macroscopic scale. Our theory predicts cell reorientation on stretched elastic
substrates.Comment: Revtex, 6 pages, 2 Postscript files included, to appear in Phys. Rev.
Let
Potential contributions of noncontact atomic force microscopy for the future Casimir force measurements
Surface electric noise, i.e., the non-uniform distribution of charges and
potentials on a surface, poses a great experimental challenge in modern
precision force measurements. Such a challenge is encountered in a number of
different experimental circumstances. The scientists employing atomic force
microscopy (AFM) have long focused their efforts to understand the
surface-related noise issues via variants of AFM techniques, such as Kelvin
probe force microscopy or electric force microscopy. Recently, the physicists
investigating quantum vacuum fluctuation phenomena between two closely-spaced
objects have also begun to collect experimental evidence indicating a presence
of surface effects neglected in their previous analyses. It now appears that
the two seemingly disparate science communities are encountering effects rooted
in the same surface phenomena. In this report, we suggest specific experimental
tasks to be performed in the near future that are crucial not only for
fostering needed collaborations between the two communities, but also for
providing valuable data on the surface effects in order to draw the most
realistic conclusion about the actual contribution of the Casimir force (or van
der Waals force) between a pair of real materials.Comment: The paper appeared in the Proceedings to the 12th International
Conference on Noncontact Atomic Force Microscopy (NC-AFM 2009) and Casimir
2009 Satellite Worksho
Deformation and tribology of multi-walled hollow nanoparticles
Multi-walled hollow nanoparticles made from tungsten disulphide (WS) show
exceptional tribological performance as additives to liquid lubricants due to
effective transfer of low shear strength material onto the sliding surfaces.
Using a scaling approach based on continuum elasticity theory for shells and
pairwise summation of van der Waals interactions, we show that van der Waals
interactions cause strong adhesion to the substrate which favors release of
delaminated layers onto the surfaces. For large and thin nanoparticles, van der
Waals adhesion can cause considerable deformation and subsequent delamination.
For the thick WS nanoparticles, deformation due to van der Waals
interactions remains small and the main mechanism for delamination is pressure
which in fact leads to collapse beyond a critical value. We also discuss the
effect of shear flow on deformation and rolling on the substrate.Comment: Latex, 13 pages with 3 Postscript figures included, to appear in
Europhysics Letter
Effect of Poisson ratio on cellular structure formation
Mechanically active cells in soft media act as force dipoles. The resulting
elastic interactions are long-ranged and favor the formation of strings. We
show analytically that due to screening, the effective interaction between
strings decays exponentially, with a decay length determined only by geometry.
Both for disordered and ordered arrangements of cells, we predict novel phase
transitions from paraelastic to ferroelastic and anti-ferroelastic phases as a
function of Poisson ratio.Comment: 4 pages, Revtex, 4 Postscript figures include
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