Endophytes vs tree pathogens and pests: can they be used as biological control agents to improve tree health?

Like all other plants, trees are vulnerable to attack by a multitude of pests and pathogens. Current control measures for many of these diseases are limited and relatively ineffective. Several methods, including the use of conventional synthetic agro-chemicals, are employed to reduce the impact of pests and diseases. However, because of mounting concerns about adverse effects on the environment and a variety of economic reasons, this limited management of tree diseases by chemical methods is losing ground. The use of biological control, as a more environmentally friendly alternative, is becoming increasingly popular in plant protection. This can include the deployment of soil inoculants and foliar sprays, but the increased knowledge of microbial ecology in the phytosphere, in particular phylloplane microbes and endophytes, has stimulated new thinking for biocontrol approaches. Endophytes are microbes that live within plant tissues. As such, they hold potential as biocontrol agents against plant diseases because they are able to colonize the same ecological niche favoured by many invading pathogens. However, the development and exploitation of endophytes as biocontrol agents will have to overcome numerous challenges. The optimization and improvement of strategies employed in endophyte research can contribute towards discovering effective and competent biocontrol agents. The impact of environment and plant genotype on selecting potentially beneficial and exploitable endophytes for biocontrol is poorly understood. How endophytes synergise or antagonise one another is also an important factor. This review focusses on recent research addressing the biocontrol of plant diseases and pests using endophytic fungi and bacteria, alongside the challenges and limitations encountered and how these can be overcome. We frame this review in the context of tree pests and diseases, since trees are arguably the most difficult plant species to study, work on and manage, yet they represent one of the most important organisms on Earth

Endophytes vs tree pathogens and pests: can they be used as biological control agents to improve tree health?

Like all other plants, trees are vulnerable to attack by a multitude of pests and pathogens. Current control measures for many of these diseases are limited and relatively ineffective. Several methods, including the use of conventional synthetic agro-chemicals, are employed to reduce the impact of pests and diseases. However, because of mounting concerns about adverse effects on the environment and a variety of economic reasons, this limited management of tree diseases by chemical methods is losing ground. The use of biological control, as a more environmentally friendly alternative, is becoming increasingly popular in plant protection. This can include the deployment of soil inoculants and foliar sprays, but the increased knowledge of microbial ecology in the phytosphere, in particular phylloplane microbes and endophytes, has stimulated new thinking for biocontrol approaches. Endophytes are microbes that live within plant tissues. As such, they hold potential as biocontrol agents against plant diseases because they are able to colonize the same ecological niche favoured by many invading pathogens. However, the development and exploitation of endophytes as biocontrol agents will have to overcome numerous challenges. The optimization and improvement of strategies employed in endophyte research can contribute towards discovering effective and competent biocontrol agents. The impact of environment and plant genotype on selecting potentially beneficial and exploitable endophytes for biocontrol is poorly understood. How endophytes synergise or antagonise one another is also an important factor. This review focusses on recent research addressing the biocontrol of plant diseases and pests using endophytic fungi and bacteria, alongside the challenges and limitations encountered and how these can be overcome. We frame this review in the context of tree pests and diseases, since trees are arguably the most difficult plant species to study, work on and manage, yet they represent one of the most important organisms on Earth

The effect of age, frontal skinfold development and season on libido and mating dexterity in Merino rams

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Die invloed van speenouderdom op die doeltreffendheid van 'n versnelde paringsfrekwensie et skape

No Abstract

Fatty acid composition and oxidative stability of lambs’ meat as affected by a bioflavonoid antioxidant and fat sources

A study was conducted to investigate the effects of a synthetic or natural antioxidant and fat saturation, in a standard feedlot diet, on fatty acid composition and oxidative stability of lamb. The four dietary treatments consisted of the same basal diet providing 187 g crude protein (CP), 355 g neutral detergent fibre (NDF), and 71 g ether extract (EE) per kg dry matter (DM), differing in fat source (30 g/kg of either saturated beef tallow or unsaturated soybean oil) and type of antioxidant included (125 g/t of either a synthetic or natural antioxidant). Eighty four S.A. Mutton Merino lambs weighing 27.6 ± 1.7 kg were divided into four groups and randomly allocated to four dietary treatments (n = 21 lambs/treatment) subdivided into 7 replicates/treatment (n = 3 lambs/replicate). After an adaptation period of 8 days, all lambs received complete diets for a further feeding period of 41 days. At termination of the study, seven lambs per treatment, weighing 45.1 ± 3.0 kg, were randomly selected and slaughtered. Loin chops from each carcass were used for fatty acid, colour (a* values) and thiobarbituric acid reactive substance (TBARS) analysis. Meat colour was determined on days 0 and 7 after being stored at 4 ºC under fluorescent light. The malonaldehyde content per kg meat was determined on days 0, 7 and 90 after being stored at -18 ºC in the dark. It was found that dietary treatment had no effect on colour stability as depicted in a* values. The malonaldehyde content per kg meat was higher on days 0 and 90 for the unsaturated soybean oil treatment. Beef tallow inclusion resulted in an increase in palmitoleic acid, where soybean oil inclusion resulted in an increase in linoleic and α-linolenic acids in both lean and subcutaneous fat tissue. Natural antioxidant inclusion in the diet only increased the palmitoleic acid content of subcutaneous fat. The results suggested that the fatty acid profile of lamb meat can be favourably manipulated by the source of fat included in the diet.Keywords: Antioxidants, dietary fat saturation, feedlot lamb

The effect of dietary lipid saturation and antioxidant source on the nutrient digestibility of lamb finishing diets

The influence of a synthetic or natural antioxidant and lipid saturation on the apparent digestibility of nutrients in a standard lamb finishing diet was investigated. The four dietary treatments consisted of the same basal diet, providing 187 g CP-, 355 g NDF- and 71 g EE per kg DM, but differing in supplemental lipid source (30 g/kg of either saturated beef tallow or unsaturated soybean oil) and type of antioxidant included (125 g/ton of either a synthetic or natural antioxidant). The digestibility study was conducted over a period of 12 days (including a 4-day adaptation to the faecal collection bags). Twenty-eight S.A. Mutton Merino lambs (45.1 ± 3.0 kg) were randomly allocated to the four dietary treatments (n = 7 lambs/treatment). Composite feed, feed refusal and faecal samples of individually penned lambs were collected for chemical analysis. The apparent digestibility coefficients, digestible nutrient and available energy content were calculated accordingly. The DMI of the lambs did not differ significantly between the various experimental diets. The inclusion of unsaturated soybean oil reduced the apparent digestibility of NDF in the diet. The apparent digestibility of NDF seems to be higher when a natural antioxidant was included in the diet. The comparatively negative effects of the unsaturated lipid source and synthetic antioxidant on the apparent digestibility of NDF were associated with a significantly lower digestible NDF content in the experimental diet. Accordingly, soybean oil resulted in a significantly lower ME content in the diet. However, estimating ME from DE with a constant factor of 0.8 probably underestimates the ME content of diets supplemented with lipids rich in unsaturated fatty acids. The latter are known to reduce methane production, and hence, energy losses.Keywords: Beef tallow, fatty acid, flavonoid, methane, soybean oi

The effect of dietary lipid saturation and antioxidant type on the performance of finishing lambs

The effect on production performance of a synthetic or natural antioxidant and lipid saturation in the finishing diets of lambs was investigated. The four dietary treatments consisted of the same basal diet (187 g CP, 355 g NDF, and 71 g EE per kg DM), differing only in regard to the supplemental lipid source (30 g/kg of either saturated beef tallow or unsaturated soybean oil) and type of antioxidant (125 g/ton of either a synthetic or natural antioxidant) included, in a 2 x 2 factorial design experiment. Eighty-four S.A. Mutton Merino lambs (27.64 ± 1.72 kg) were randomly allocated to the four dietary treatments (n = 21 lambs per treatment) and subdivided into 7 replicates per treatment (n = 3 lambs per replicate). After a dietary adaptation period of 8 days, all lambs received the respective experimental diets for the remaining period (41 days). The average daily DM feed intake, weight gain and feed efficiency was calculated accordingly. No significant differences in DMI, ADG and the efficiency with which ingested feed were utilized (FCR), were recorded for the treatments. However, the addition of unsaturated soybean oil to the diet significantly increased the efficiency with which the ME of the diet was utilized. In contrast with the natural antioxidant, the inclusion of unsaturated soybean oil in the diet containing a synthetic antioxidant, resulted in a significant lower MEI by the lambs – indicating that a lipid x antioxidant interaction occurred. Results of the present study seem to indicate that dietary lipid saturation in the finishing diets of lambs had no influence on their growth performance. However, a more efficient utilisation of ME in the finishing diet containing unsaturated soybean oil, compared to the saturated beef tallow, did occur.Keywords: Fatty acid, flavonoid, metabolizable energy, oil, production, sheep, soybean, tallo

Active fungal communities in asymptomatic Eucalyptus grandis\textit {Eucalyptus grandis} stems differ between a susceptible and resistant clone

Fungi represent a common and diverse part of the microbial communities that associate with plants. They also commonly colonise various plant parts asymptomatically. The molecular mechanisms of these interactions are, however, poorly understood. In this study we use transcriptomic data from $\textit {Eucalyptus grandis}$, to demonstrate that RNA-seq data are a neglected source of information to study fungal–host interactions, by exploring the fungal transcripts they inevitably contain. We identified fungal transcripts from $\textit {E. grandis}$ data based on their sequence dissimilarity to the $\textit {E. grandis}$ genome and predicted biological functions. Taxonomic classifications identified, amongst other fungi, many well-known pathogenic fungal taxa in the asymptomatic tissue of $\textit {E. grandis}$. The comparison of a clone of $\textit {E. grandis}$ resistant to Chrysoporthe austroafricana with a susceptible clone revealed a significant difference in the number of fungal transcripts, while the number of fungal taxa was not substantially affected. Classifications of transcripts based on their respective biological functions showed that the fungal communities of the two $\textit {E. grandis}$ clones associate with fundamental biological processes, with some notable differences. To shield the greater host defence machinery in the resistant $\textit {E. grandis}$ clone, fungi produce more secondary metabolites, whereas the environment for fungi associated with the susceptible $\textit {E. grandis}$ clone is more conducive for building fungal cellular structures and biomass growth. Secreted proteins included carbohydrate active enzymes that potentially are involved in fungal–plant and fungal–microbe interactions. While plant transcriptome datasets cannot replace the need for designed experiments to probe plant–microbe interactions at a molecular level, they clearly hold potential to add to the understanding of the diversity of plant–microbe interactions